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Offlinehookem
Stranger
Registered: 02/21/06
Posts: 8
Last seen: 17 years, 7 months
quick question about inoculation
    #5338394 - 02/25/06 03:52 PM (17 years, 11 months ago)

Hey I posted another question about a week ago regarding inoculation and colonization, thanks to those who replied, and I have one more question before I go for my second try.
Last time, when I was innoculating my cakes, I noticed that the syringe was getting vermiculite stuck inside the needle. None of my cakes colonized at all. I was just curious if there is any chance that the vermiculite in the syringe could have caught the spores and not allowed them to pass through with the water? Has anyone else had this problem?


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OfflineDICK
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Registered: 12/28/05
Posts: 555
Last seen: 15 years, 7 months
Re: quick question about inoculation [Re: hookem]
    #5338407 - 02/25/06 03:58 PM (17 years, 11 months ago)

well I supposed if it didnt let any liquid pass the syringue would be completely clogged as in putting pressure on it would accomplish nothing... know what I mean? But as long as you saw your cc's go down and plunger subside then the tiny obstruction wasnt a problem... Id suggest throwing away the needle if you cant get the verm out since the little particle could be a carrier to contamination. Or if you have other empty syringues fill it with water and try to 'shoot' the verm away.


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Offlinehookem
Stranger
Registered: 02/21/06
Posts: 8
Last seen: 17 years, 7 months
Re: quick question about inoculation [Re: DICK]
    #5338584 - 02/25/06 05:33 PM (17 years, 11 months ago)

But as long as the liquid passes through it shouldn't be a problem?


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Offlinecoda
Banjo Goiter
Male

Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
Trusted Cultivator
Re: quick question about inoculation [Re: hookem]
    #5339191 - 02/25/06 08:40 PM (17 years, 11 months ago)

yup, you'll be fine, as long as you got your needle down past the dry verm layer you'll be ok.


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

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i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



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InvisibleliloldmeFacebook
( ͝° ͜ʖ͡°)つ=D
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Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
Trusted Cultivator
Re: quick question about inoculation [Re: coda]
    #5339284 - 02/25/06 09:06 PM (17 years, 11 months ago)

Coda is right, if it bothers you still just poke a needle into the syringe to get it out


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