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OfflineJon
Registered: 06/28/03
Posts: 961
Last seen: 9 years, 5 months
What would happen if...
    #5337617 - 02/25/06 10:52 AM (18 years, 2 months ago)

What would happen if you placed a piece of cloned material on one end of an agar plate, and spores on the other and allowed it to colonize?

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Invisibleshymanta
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Registered: 01/27/05
Posts: 907
Re: What would happen if... [Re: Jon]
    #5337646 - 02/25/06 11:10 AM (18 years, 2 months ago)

Each sub-strain would compete for nutrients. My guess is that the clone would have the advantage. It wouldn't have any competition on its side of the plate; thus would grow faster and dominate the plate. Then I think you would see a line between the clone and multi-spore mycelium.

I've often wondered if the mycelium of the same species would cannibalize the weaker of the two, taking over the substrate.

And, I think you saw this coming, try it and see. Then post results.

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Invisibleshroomydan
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Re: What would happen if... [Re: Jon]
    #5337674 - 02/25/06 11:24 AM (18 years, 2 months ago)

I think they would fuse into a single colony.

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Offlinecoda
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Re: What would happen if... [Re: shroomydan]
    #5338488 - 02/25/06 04:54 PM (18 years, 2 months ago)

i think the tissue would create mycellium faster then the MS innoc. Ive seen agar plates that had two strains on them, there was a clear divide in the middle of the plate between the two strains.


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To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.

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OfflineRogerRabbitM
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Re: What would happen if... [Re: coda]
    #5340461 - 02/26/06 09:54 AM (18 years, 2 months ago)

Here's the line of isolation that will develop.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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InvisibleDragonaut

Registered: 06/24/04
Posts: 6,219
Re: What would happen if... [Re: RogerRabbit]
    #5343016 - 02/26/06 09:07 PM (18 years, 2 months ago)

line of isolation...or zone of inhibition?


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:dragon:

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OfflineRogerRabbitM
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Re: What would happen if... [Re: Dragonaut]
    #5344406 - 02/27/06 07:54 AM (18 years, 2 months ago)

Left turn, or three rights? Same thing.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Invisibleohmatic
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Re: What would happen if... [Re: RogerRabbit]
    #5344459 - 02/27/06 08:12 AM (18 years, 2 months ago)

Quote:

RogerRabbit said:
Left turn, or three rights?  Same thing.
RR




hehe i like that :smile:


*edit
thats 2 wedges of the same clone
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

Edited by ohmatic (02/27/06 08:13 AM)

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Offlinethenewguy05
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Registered: 02/11/05
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Re: What would happen if... [Re: ohmatic]
    #5344676 - 02/27/06 09:52 AM (18 years, 2 months ago)

those are both very rhyzomorphic...nice choices for isolation.

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OfflineRogerRabbitM
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Re: What would happen if... [Re: thenewguy05]
    #5344768 - 02/27/06 10:24 AM (18 years, 2 months ago)

Nice pic ohm. That shows how you can see the two identical cultures growing together. The one I posted above is of different strains, therefore you can see a line of bare agar between them.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Invisibleohmatic
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Re: What would happen if... [Re: RogerRabbit]
    #5344805 - 02/27/06 10:33 AM (18 years, 2 months ago)

you know what i just thought, basically the line cant really be of bare agar.

reason i think that is, if it were of bare agar the two culture would not know of
each others existance and therefor continue growing - but as they know, they dont.

therefor there must actually be some mycelium of both cultures in that "bare" strip,
despite being invisible or hardly visible.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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OfflineRogerRabbitM
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Re: What would happen if... [Re: ohmatic]
    #5345452 - 02/27/06 01:51 PM (18 years, 2 months ago)

See http://www.mushroomvideos.com/868246.html and look at video 3. Look how far away the mycelium is from the contaminant when it begins to 'circle the wagons'. There was no mycelium in the no mans land, so I have no idea how the reishi knew of the presence of the contaminant-I can only speculate about harmones, scents or actual sentience. However, you are correct. In the picture I posted above, there are small, invisible to the naked eye, mycelial strands that reach out ahead of the growth(I call them 'scouts') and when those mingle, they stop the respective growths as a survival tactic. It's these 'scouts' that are harvested once they make contact for hybridization experiments, when one is attempting to breed dikaryons. I think it's interesting how the more we learn, the more we realize how little we still actually know about our beloved fungi.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Invisibleohmatic
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Re: What would happen if... [Re: RogerRabbit]
    #5345671 - 02/27/06 02:53 PM (18 years, 2 months ago)

Quote:

RogerRabbit said:
See I think it's interesting how the more we learn, the more we realize how little we still actually know about our beloved fungi.
RR




:heart: :sun: :heart:

yes, it proves so every day.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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InvisiblePsychoChipmunk
Small, Furry, Disturbed

Registered: 01/08/05
Posts: 389
Loc: A hole in your back yard
Re: What would happen if... [Re: RogerRabbit]
    #5367068 - 03/05/06 11:09 AM (18 years, 2 months ago)

Video number 5 (reishi fruiting from substrate bags) was really cool. Interesting how the initial growth out of the bag almost pulsates, growing and resting alternately.


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\m/

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