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Invisibleremediator
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Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
New Orleans soil and fungi....
    #5332012 - 02/23/06 02:57 PM (18 years, 2 months ago)

Hey all.
Been a regular here before, now i have a renewed energy and slightly different focus for my cultivation efforts.

Looking for suggestions and advice on using gourmet and medicinals for use in bioremediation efforts here in the post-Katrina Crescent city.

I have excellent sterile technique skills and lots of experience and I'm currently simply seeking comments, discussion and advice on removing toxins from the soil using mushrooms.
Of course, dishes and or spawn would be a boon, as I'm working with the non-profit Common Ground collective at the moment, and we're starting with playgrounds and schools, but intend to move to backyards and such.
Any information about specific capabilities of individual species to remove/metabolize/concentrate lead, arsenic, diesel fuel derivatives, and other heavy metals is welcomed.

Some starter questions include:

1. Which species of mushroom that is commonly cultivated currently is most efficient at the removing the largest number of toxins?

2. What kind of ratio of substrate to earth would one need for tilling down 8-10 inches and introducing wood-loving spawn - ie. oysters?

3.Any good suggestions for disposing of fruitbodies that are theoretically contaminated? Lead is pretty persistent...

/anyway, i hope this post is well- received here. Mods by all means step in and redirect me if necessary.

hope to hear from anyone on this, as this project is worthy of tons of effort and hopefully can serve as a hands on myco-remediation experience for lots of eager homeowners, educators and regular hippy folk as well...
peace

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OfflineAnnoA
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5332496 - 02/23/06 06:02 PM (18 years, 2 months ago)

Welcome,

There is a new book, where you will find the answers to most if not all of your questions:

Mycelium Running: How Mushrooms Can Help Save the World

Quote:

A manual for healing the earth and creating sustainable forests through mushroom cultivation, featuring mycelial solutions to water pollution, toxic spills, and other ecological challenges.
Mycotechnology is part of a larger trend toward using living systems to solve environmental problems and to restore ecosystems.
Covers mycorestoration (biotransforming stripped land), mycofiltration (creating habitat buffers), mycoremediation (healing chemically harmed environments), and mycoforestry (creating truly sustainable forests).
More than 300 full-color photographs.



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Offlinekatshroomer
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Re: New Orleans soil and fungi.... [Re: Anno]
    #5340234 - 02/26/06 06:58 AM (18 years, 2 months ago)

I have experience using mycological micofiltration and micoremediation. I'm in the area regularly. PM me.

Some areas of NOLA could use a healthy does of lignin peroxidases.

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Offlinepocketmulch
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5344214 - 02/27/06 04:57 AM (18 years, 2 months ago)

I'll second the recommendation on Stamets' new book.

> 2. What kind of ratio of substrate to earth would one need for tilling
> down 8-10 inches and introducing wood-loving spawn - ie. oysters?

Stamets recommends (in GGMM) a 5-50% inoculation rate for outdoor patches, with best experiences at 20%. But you'd probably be fine going on the lower end if you're not trying to maximize edible fruitbody production.

Keep us updated on the project! I used to live in and still love NOLA, and would love to go back to a city restored by mushrooms.

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OfflineRogerRabbitM
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Re: New Orleans soil and fungi.... [Re: pocketmulch]
    #5344451 - 02/27/06 08:09 AM (18 years, 2 months ago)

The experiments I've seen had substrate prepared and inoculated in burlap bags that were then layed side by side to completely cover the affected area. They eventually break down and become part of the soil below.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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InvisibleStonerguy
I smoke penis
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5345635 - 02/27/06 02:40 PM (18 years, 2 months ago)

I don't think I can help you out any, but I just wanted to give you thanks for using your own time to help people in New Orleans.

+5 :mushroom2: for you


--------------------
yawn...
SG

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Invisibleremediator
IllustriousPotentato
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Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
Re: New Orleans soil and fungi.... [Re: Stonerguy]
    #5345943 - 02/27/06 03:56 PM (18 years, 2 months ago)

Ahhh. Thanks for the approval and suggestions. I own all of P.S. books except for Mycelium Running, tad shameful i must admit at this point.

But things are working out.
There is a vested interest now in long term commitment, so there will be an effort to get a small lab going, and there is an effort underway to get donations from nearby production facilities in the form of spent substrate.
Things are happening. I plan to start teaching and producing oyster spawn later this week...
Glad to hear the shout-outs to NOLA... werd, this place needs some serious help all over...

Thanks Anno - forever wise and straightforward.
Thanks Kat - check yer pms
thanks Pocketmulch - I was thinking 5:1 no less than 8:1 for "millet-spawned straw" to soil.
Thanks Rodge - I was actually thinking of this too. But considering spawning rows of straw/chips with grain, incubating for a couple of weeks, then tilling into the soil...
Any comments on that?
And yes, a copy of the book is on it's way...
peace

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OfflineRogerRabbitM
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5346154 - 02/27/06 04:43 PM (18 years, 2 months ago)

I doubt oyster spawn will survive below soil level. Try it first on a small scale.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinekatshroomer
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Re: New Orleans soil and fungi.... [Re: RogerRabbit]
    #5350728 - 02/28/06 07:24 PM (18 years, 2 months ago)

Now that I think about it...

I think Common Ground is already working on bioremediation in NOLA, you may want to check with them. They're pretty concentrated in the 9th ward though.

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Invisiblemycofile
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Re: New Orleans soil and fungi.... [Re: katshroomer]
    #5353646 - 03/01/06 01:42 PM (18 years, 2 months ago)

Yeah, all of the bio-remediation with oysters that I can remember used "bunker spawn" (burlap bags) or mounds.

A note about the spawn rate, particularly in mounds, stamets says he has seen much better results when layering the spawn rather than thorough mixing, although that is in contrast to what many would do in the lab, it works better in the field.

And if you are even considering actually trying to do any bio-remediation without reading Mycelium Running, you are needlessly swimming against the current....Pick it up from the shroomery bookstore, it's only like $20 something, add another $2 book and shipping is free.....


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Invisibleremediator
IllustriousPotentato
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Loc: NOLA ATM
Re: New Orleans soil and fungi.... [Re: mycofile]
    #5353758 - 03/01/06 02:29 PM (18 years, 2 months ago)

The book will be here by tomorrow, guys. just had to wait on shipping.

And Common Ground is definitely who i am working with to get this going. The picture is finally emerging that cultivation of mushrooms on site is crucial to the majority of the projects they are undertaking. There are sites waiting for spawn, and we are waiting to hear about some donations currently of spent substrate blocks from nearby farms...

It looks like I'm going to be designing a Lab/incubation room/grow house
to have on site, and that they'll be able to afford a nice hood and some fans and grow house gear...

I guess with The Mushroom Cultivator, Growing Gourmet and Medicinal Mushrooms, and Mycelium Running I'll have most of the data i need.

But there is no replacement for hands on experience.

My reasons for shying away from layering the substrate on top of the beds would be theoretically that i would simply be building soil on top of the contamination, and that without some help with penetration the oysters would likely colonize all the lignicolous matter and then go into fruiting.
Plants then grown on this bed would seek nutrients deeper down and come up with arsenic and dealdrin and lead anyway.

So I'm thinking heavy inoculation rates and "some" tilling under the beds...

Thanks for all your kind comments, and i look forward to hooking up with new orleanians who have any experience or want to learn some stuff.

Efforts are underway to begin brewing large quantities of compost tea, we are considering making our own EM, and the spawn production begins in earnest very soon.
PEACE to all.
Had a sick-ass Mardi Gras, BTW. I love this place

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OfflineRogerRabbitM
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5354714 - 03/01/06 07:42 PM (18 years, 2 months ago)

If you haven't already, you might want to consider the Mycorestoration seminar with paul stamets. It's in june. Scroll down the page to the Mycorestoration Seminar from the link below.
http://www.fungi.com/seminars/index.html
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineAnnoA
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5357014 - 03/02/06 10:53 AM (18 years, 2 months ago)

You might also write him an email, and tell him about your plans, I am sure he will be interested.

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InvisibleTsinaglou
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5359666 - 03/02/06 09:38 PM (18 years, 2 months ago)

If soil has some pore space and air exchange/moisture flow chemical degrading enzymes percolate from the mycelium down into the soil. Humic acid generated by a mulch layer helps cause soil aggregation and larger pore spaces.

I suspect the strongest generalist wood digesters would also break down the most organic petrochems. Pleurotis and Trametes being high on the list.

Radishes are sometimes used to open up a dense soil. Grow long-rooted types. Hypsizygus ulmarius (elm oyster mushroom) seems to like growing around the roots of radish/cabbage/collard family plants, resulting in deeper soil penetration of both the roots and mycelium. They natively grow on wood far from the soil so root symbiosis is a suprise. In Paul Stamets experiment with them he used sawdust spawn spread in and on the soil. They increased yeild of associated plants while Pleurotis decreased yields of green plants.

Isn't jimson weed grown to pull arsenic out of soil? I vaguely recollect hearing that it pulls heavily on some toxic minerals...

It's an untried experiment as far as I know, but combining mushroom spawn with deep-rooted grasses might give the best soil penetration.

Best of luck!
Tsin

Quote:

remediator said:
My reasons for shying away from layering the substrate on top of the beds would be theoretically that i would simply be building soil on top of the contamination, and that without some help with penetration the oysters would likely colonize all the lignicolous matter and then go into fruiting.
Plants then grown on this bed would seek nutrients deeper down and come up with arsenic and dealdrin and lead anyway.

So I'm thinking heavy inoculation rates and "some" tilling under the beds...



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Invisibleremediator
IllustriousPotentato
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Registered: 02/23/06
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Loc: NOLA ATM
Re: New Orleans soil and fungi.... [Re: Tsinaglou]
    #5361232 - 03/03/06 11:28 AM (18 years, 2 months ago)

Wow, thanks Tsinaglou!!!
Very encouraging information...
My expertise is in sterile technique and spawn production, but most of the folks I'm around all day are permaculture/organic gardening folks who will love the information...
Hopefully i'll be reading Mycelium Running by later this afternoon...

peace
Remediator/Viscid/Chickenofthewoods

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Invisibleremediator
IllustriousPotentato
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Registered: 02/23/06
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Loc: NOLA ATM
Re: New Orleans soil and fungi.... [Re: remediator]
    #5376920 - 03/08/06 09:06 AM (18 years, 2 months ago)

hey all.
maybe this will get some discussion going...

Yesterday i made a mycelial slurry from a fresh P pulmonarius fruit.
(Glovebox - sterile chunk from inside the stem - sterile water/broken glass)
The master containers were yeast/ground millet/honey and the jars were fitted with homemade silicone injection ports, the idea being that it would be possible, with flame sterilized needles, to carry out inoculations of sterile quart grain jars outside of the glove box.
I made myself an "airport" filter from a spare syringe and some 3M micropore tape covering the needle outlet nozzle in the syringe body, and then taped a patch of tyvek around the back of the syringe as a secondary precaution in case the tape adhesive failed me.

Now heres the clincher. I know that sterile techniques are simply the best way to produce large quantities of spawn, and i want to help these folks get that accomplished with as little effort and expenditure as possible...
I really want a pre-packaged liquid product that i can inoculate with Liquid Culture via the airport/silicone port method to facilitate the ease of inoculations.
I bought a couple of bottles of the Glaceau Peach flavored energy water, which has 5g sugar per 240 ml, which i calculated as 2.35 percent sugar BY WEIGHT, considering that water weighs roughly 1 oz. per fluid oz.
Which brings me to this:
Has anyone had success with this yet?
My attempt ended abruptly when the masking tape i was using to adhere to the plastic bottle to support the silicone just sort of popped off. I'm thinking fingernail file to roughen the spot then go with the silicone directly onto the bottle.
This method, if perfected, could be a simple solution to the difficulty most people have with sterile technique, ie. grokking the whole picture takes experience, time and patience, all of which are at a minimum in this disaster area i call home...

We've got all kinds of sources for bulk substrate, but few of them are fresh or all that useful. Trying to hook up with some sugar cane bagasse. I'm thinking that with enough liquid mycelium from clones, i can inoculate 5 gallon buckets (not full) of Coffee grounds and chaff to build up the spawn for beds of cardboard/mulch and to spawn some bunker bags.
Waiting on some 5 lb bags of spawn from MushroomPeople that a friend ordered to make my grain masters, i have about 40 jars ready to get knocked up. Hoping today to get some little plastic ramekins with lids from a restaurant to start some agar.
As the project builds momentum, i see the need for a diversity of species emerging and was wondering if it would be terribly inappropriate to request assistance here? Phanerochaetes chrysosporium and some of the non-fruiting delignifiers would be nice to have on hand. Trametes and king stropharia as well as ganoderma spp and laetiporus may do well here...
ANY HELP, SUGGESTIONS, QUESTIONS, DONATIONS, OR SUPPLIES ARE WELCOME AND ENCOURAGED.

LOVE
REMEDIATOR

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OfflineBLUEDOG
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5377696 - 03/08/06 02:24 PM (18 years, 2 months ago)

If the mushrooms are being used for bioremediation, how do you dispose of the fruits. I wouldn't want to eat them since they would be full of heavy metals, arsenic, etc. Would you burn them and dispose the ash as toxic waste. Just a thought.
Nola needs alot of help. I will be there for jazzfest and hopefully help with whatever I can.

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Invisibleremediator
IllustriousPotentato
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Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
Re: New Orleans soil and fungi.... [Re: BLUEDOG]
    #5382864 - 03/09/06 08:11 PM (18 years, 2 months ago)

Bluedog -
Certain species hyper-accumulate certain heavy metals, while avoiding uptaking others entirely or at least almost...
Everything depends on soil test results at specific locations.
If there are no significant heavy metals, the wide range of petroleum hydrocarbons are largely consumed by the mushrooms, which are typically going to be oysters.  I certainly wouldn't want to eat the mushrooms from any contaminated area at all, but i wouldn't mind eating vegetables grown in the same area after a thorough cleanup with compost tea and some saprophytes.
If in fact you ARE dealing with persistent accumulative metals like cadmium and arsenic, there are specific mushrooms that like those too.
For instance, Shaggy Manes (Coprinus comatus) will concentrate cadmium, arsenic and mercury, but very little in the way of lead, copper or cesium.
These mushrooms could be used in specific contamination scenarios to remove arsenic, but of course the arsenic would be in the fruitbodies.  Not broken down.  So harvesting and disposing of the mushrooms would be :rolleyes: part of the plan then.  Incineration is the probable outcome for heavy metal contaminated fruitbodies from a mycoremediation project...Don't ask me where to incinerate hundreds of pounds of arsenic laden shaggies, though, cuz i don't know.

Does anyone know where that might be possible?

Paul Stamets in Mycelium Running even suggests that the metals might be recovered from the incineration process...although I'm not too sure about how much there is to recover....unless you've got mass acreage.

(Of course you realize I'm regurgitating stuff from his new book, you should get it, it's as transformative and powerful as GGMM.  It's well-written and pretty, too.  Perty pitchers.

Oh.  Today I turned my glovebox into a positive pressure box with computer fans found on the side of the road...  Planning to use one of them there Tyvek suits to cut out for my filter tomorrow. 
Was hoping to get some spawn today finally, but i guess it's not here yet.  Hopefully my P. Pulmonarius LC will take off and I'll have P. ostreatus and P. pulmonarius to get me started down here.  My jars are just waiting.....
Please pipe in at any time here folks.
i could use anything at all.

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InvisibleOldSpice
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Re: New Orleans soil and fungi.... [Re: Anno]
    #5384121 - 03/10/06 01:50 AM (18 years, 2 months ago)

Tons of drama in this place *steps away


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:

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Invisibleremediator
IllustriousPotentato
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Re: New Orleans soil and fungi.... [Re: OldSpice]
    #5398229 - 03/14/06 09:09 AM (18 years, 2 months ago)

Is posting a link to another thread cross-posting, if it's my own thread that i'd like to call attention to?

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Offlinefalcon
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5400808 - 03/14/06 07:55 PM (18 years, 2 months ago)

Google cache about growing oysters on pine. In this study they grew Ophiostoma piliferum on pine chips to remove the extractives, to prepare the wood for the oysters.

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Invisibleremediator
IllustriousPotentato
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Loc: NOLA ATM
Re: New Orleans soil and fungi.... [Re: falcon]
    #5402830 - 03/15/06 10:33 AM (18 years, 2 months ago)

Thank you so much... Fascinating.
What an awesome contribution.
How could i have missed the idea for so long?

--------"After conifer chip treatment with extractive-
degrading fungi, rapid and heavy filamentous growth
occurred. Ophiostoma piliferum (Cartapip 97) was
selected for large-scale treatment of conifer chips
because it is easily obtained in large quantities and
simple to use. This treatment removed 30% to 90 % of
the extractives within 4 to 5 days and induced
excellent mycelial growth and mushroom production. "

Jesus!
Where can i get some Ophiostoma piliferum?
/googling for "culture library"
//asks stupid questions when he's excited

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Invisibleremediator
IllustriousPotentato
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Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
Re: New Orleans soil and fungi.... [Re: remediator]
    #5402853 - 03/15/06 10:46 AM (18 years, 2 months ago)

Ophiostoma @ ATCC No pilferum...

Geez.
You have to apply for a permit from the USDA to order these cultures...
shizzles

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Offlinefalcon
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5406002 - 03/15/06 09:28 PM (18 years, 2 months ago)


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Invisibleremediator
IllustriousPotentato
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Posts: 137
Loc: NOLA ATM
Re: New Orleans soil and fungi.... [Re: falcon]
    #5406235 - 03/15/06 10:11 PM (18 years, 2 months ago)

Holy shit will that make it through customs?
/Hoy shit do you see the other charges?
//Holy shit "Euro 430.00  for shipment to the USA."

:poop: :glittershitz:

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Offlinefalcon
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5410577 - 03/16/06 10:52 PM (18 years, 2 months ago)

I don't know if it would make it through customs. I didn't look at the price, just the one on the front page, holy shit.

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Invisibleremediator
IllustriousPotentato
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5425018 - 03/21/06 12:12 PM (18 years, 2 months ago)

Hi all!

I've been hesitant to update, as there's not much going on at the moment.
I'm somewhat frustrated at the loss of some fresh spawn to a compost heap via "lack of awareness"
So i have sterile jars just sitting around drying up.

I made the P pulmonarius LC from a flesh clone that didn't break up well in the mother jar, and it seems they are not taking...
I did a ghetto peroxide clone of some ostreatus from a restaurant straight to grain (since they are just drying up...) and two of the four have gone so far...
i shook the other two today.
I've never been so anxious to work and had so little to work with.

On a brighter note,  the generous folks here at the Shroomery have brightened my prospects with donations, which i hope to start recieving before the week is up.
MycoShack has been crazy mad helpful... offering dishes and cultures and other necessities as well as offering to build a flow hood for us!!!
Way to go guys, thanks a bunch.
I can't wait to get some agar poured.
Jeremy Davis has offered a HEPA filter, and his time and services as well, and i hope we can arrange to have him come up from florida to help us with some demonstrations...He does some awesome  work  himself at this non-profit...
Falcon said he would send some cultures of Hypsizugus tessulatus, which is an awesome companion plant.
Pocketmulch offered up some P djamor and columbinus...
Would like to have Trametes, as it's highly effective at getting some of the real nasties, like dieldrin and aldrin. 
I am currently researching Trichoderma as a possible remediator, and looking hard at Phanerochaetes chrysosporium, anybody seen that guy?

Sorry i don't have any pretty pics or more exciting news, soon, soon.

We may have a lab space soon, too.  The supplies need a house. 

Also Tulane university may become involved, if i have anything to say about it...
peace to you all, and thanks to everyone who has participate in the discussion.  Things are moving forward.  i have high hopes that we can grow tons of mushrooms and clean up this place at the same time, it's my dream...
:smile:

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InvisibleDarkenshroom
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5428932 - 03/22/06 08:05 AM (18 years, 2 months ago)

I am glad to hear that things are looking up down there. I am growing more and more interested in your project. In fact it has prompted me to buy Growing Gourmet and Medicinal Mushrooms and Mycelium Running.

I found the thread you had mentioned earlier that you were concerned over linking. Prospective Liquid for LC's in New Orleans Resto Project?

Is that the one? ^_~ I posted in that thread the only questions I would have regarding using that.

I sincerely wish you the best down there, what you are doing is a great thing. Also I agree with the poster who mentioned contacting Stamets, with his newest book and insights into Mushroom Cultivation being related to the very endeavor you are attempting I would not doubt that he would have an interest.

May your endeavor gain steam and be fruitful (quite literally)!

Darken
*smiles*


--------------------
~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~

Edited by Darkenshroom (03/22/06 08:06 AM)

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Invisibleremediator
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Re: New Orleans soil and fungi.... [Re: remediator]
    #5474038 - 04/03/06 10:11 AM (18 years, 1 month ago)

Well, I thought I'd give you guys a little heads up.

Sort of a pre-update update.

Things are moving along, albeit still too slowly.
The weather is getting summery and the volunteers i interact with are leaving and being replaced by new faces.

But i have poured some agar.

I have transferred in the last week:
Gill tissue of Macrolepiota rachodes from a local compost pile.
agar wedges of Hypsizygus tessulatus from Falcon.
" " Neolentinus ponderosus " "
" " Stropharia Rugoso-annulata " "

And some oysters from a local restaurant.

And there is a space for the Lab reserved for the Bio-Crew at the ArtEgg, which is a local Art Studio borne of a remodelled egg and butter warehouse... I have heard that the space is 20' X 50' with no windows. And ceiling fans? I'm going to check it out today and write up my proposal for the use of the room...
In which case I'm going to want to go ahead with the Flow Hood...

I have a 256cfm squirrel fan at my disposal now. I also have the tools and materials aside from the HEPA, which has been offered by Mr. Jeremy Davis
of the Shroomery.

So there are some jars on the way. I put some H tessulatus directly into millet quarts, so we'll see how fast i can get some beds going now. Sadly enough, none of the "spent substrate" leads panned out. I haven't had time myself to track down farms nearby...yet.

Uhm. Lemme see,
After i check the mail today and visit the artegg, I'll let you guys know how things are going....

peace and thanks to all who have helped with their time and supplies.

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Offlinejlocke85
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Registered: 12/05/05
Posts: 113
Loc: MI
Last seen: 16 years, 8 days
Re: New Orleans soil and fungi.... [Re: remediator]
    #5481710 - 04/05/06 06:27 AM (18 years, 1 month ago)

I'm glad to hear things are at least moving along somewhat down there. Have you checked out any of the spawn at the garden? There were two buckets of oyster that were fully colonized, one of which was fruiting. Then there are three cardboard spawn piles, although they could be made thicker so they don't dry out as easily. They seemed to be doing pretty good when I left, but who knows what has happened. Its likely the haven't been watered much since I left. Hopefully some new dedicated volunteers will come through soon and continue to usher the project along.

- Joe

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OfflineJeremy_Davis
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Registered: 04/22/05
Posts: 652
Loc: Florida
Last seen: 12 years, 1 month
Re: New Orleans soil and fungi.... [Re: jlocke85]
    #5490860 - 04/07/06 07:31 PM (18 years, 1 month ago)

Did you try Quincy Farms in N. Florida, they're outside Talahassee. Here's their website for their spent compost. I bet you can get it by the tractor trailer full;

http://www.quincycompost.com/

Light and Love,
JD


--------------------
Jeremy Davis
Educational Concerns for Hunger Organization, Inc.
Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta

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Invisibleremediator
IllustriousPotentato
Male

Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
Re: New Orleans soil and fungi.... [Re: Jeremy_Davis]
    #5520842 - 04/16/06 12:18 PM (18 years, 1 month ago)

Ahhhh.
Easter Sucks....

So, I'm gearing up for some beds now.  I went ahead and ordered some cultures, as the donations seemed to not flood in as much as offers...

Thanks to Falcon, i have the King Stropharia and Hypsizygus tessulatus in quantity, at least. 

I ended up using two ounce plastic ramekins for agar work, seems to work just fine - never recieved any petris. :frown:

But everything is doing well.  I have about 75 quarts of various species, including a Pleurotus that i ghetto-cloned straight to liquid and subsequently successfully got to pin in-vitro....
So those things look good.
The room for the lab is there, but i have stalled on jumping on it, simply due to the utter filthyness of the place.  I'm not sure i could get it clean and workable without tons of materials and labor.  I'm considering just building an enclosure in the space to house the flow hood. Maybe i'll filter the air in the room heavily, and have positive pressure in the whole lab, too.  Up to this point the still air box has done me well.

I would like to hear back from Mycoshack and pocketmulch...

Jeremy Davis is waiting for me to call him, I'm sure...waiting on coordination... I would like to drive down sometime and see how you folks are doing things, and maybe pick up the supplies you have offered me, so I'm trying to work that out.

All in all, I'm disappointed in the relatively little I've produced in such a long spread of weeks, but things are definitely at a hot turning point.  I've got lots of spawn running here, and the first large project is quickly becoming a reality, so I'm excited.

Maybe I'll snap some shots of the pulmonarius fruiting from the test jar, and some of my ramekin petris to show off, but there's really not much to look at.  I plan to document the projects on site relatively thoroughly though, so I'll get some eyecandy going soon enough...

Hey.  Thanks to the shroomery I've gotten started at least.  Made a great friend locally, Made some contacts that could prove fruitful for a variety of reasons, and I'm learning a lot about myself...
Hopefully I'll be eating vegetables from a new orleans garden late next year, and feel safe doing so...

Peace to all of you, and I'm always looking for links to information about specific organisms that have potential applications in bioremediation...
chickenofthewoods at yahoo dot com.

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OfflineJeremy_Davis
Mycelial NetworkAdministrator
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Registered: 04/22/05
Posts: 652
Loc: Florida
Last seen: 12 years, 1 month
Re: New Orleans soil and fungi.... [Re: remediator]
    #5526171 - 04/17/06 06:15 PM (18 years, 1 month ago)

I never got your address, and I'm moving to PA for a new job, so I've been unbelievably busy and swamped by life and all. I apologize. I PM'd you with the specifics, so we can get you up and running before I take off for PA...Baby just turned 2, doing cultivation classes every weekend, I'm working 7 day weeks, and lost or misplaced your address and number!!!
I can get my HEPA out of storage this week. I imagine the shipping is gonna be a bit much for the items we'll send so if ya can split it with me it'd be way cool. I'll send it out from the office next week if you promise to keep e-mailing me reminders!!! I also only check the boards once or twice a week right now because of the work load and pressures of packing and moving everything I own all by my lonesome as well as training new volunteers to run the ECHO projects in my stead. I know I need some time management skills! Oh and I'm still working on my degree, so I'm still in classes too. At 28 you'd think I'd be more together, but hey that's life. Never a dull moment! I always marveled at people who have time to be bored.
Light and Love,
Jeremy Davis


--------------------
Jeremy Davis
Educational Concerns for Hunger Organization, Inc.
Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta

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