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falcon


Registered: 04/01/02
Posts: 8,049
Last seen: 3 hours, 36 minutes
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Re: New Orleans soil and fungi.... [Re: remediator]
#5400808 - 03/14/06 07:55 PM (18 years, 2 months ago) |
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Google cache about growing oysters on pine. In this study they grew Ophiostoma piliferum on pine chips to remove the extractives, to prepare the wood for the oysters.
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remediator
IllustriousPotentato


Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
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Re: New Orleans soil and fungi.... [Re: falcon]
#5402830 - 03/15/06 10:33 AM (18 years, 2 months ago) |
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Thank you so much... Fascinating. What an awesome contribution. How could i have missed the idea for so long?
--------"After conifer chip treatment with extractive- degrading fungi, rapid and heavy filamentous growth occurred. Ophiostoma piliferum (Cartapip 97) was selected for large-scale treatment of conifer chips because it is easily obtained in large quantities and simple to use. This treatment removed 30% to 90 % of the extractives within 4 to 5 days and induced excellent mycelial growth and mushroom production. "
Jesus! Where can i get some Ophiostoma piliferum? /googling for "culture library" //asks stupid questions when he's excited
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remediator
IllustriousPotentato


Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
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Re: New Orleans soil and fungi.... [Re: remediator]
#5402853 - 03/15/06 10:46 AM (18 years, 2 months ago) |
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Ophiostoma @ ATCC No pilferum...
Geez. You have to apply for a permit from the USDA to order these cultures... shizzles
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falcon


Registered: 04/01/02
Posts: 8,049
Last seen: 3 hours, 36 minutes
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Re: New Orleans soil and fungi.... [Re: remediator]
#5406002 - 03/15/06 09:28 PM (18 years, 2 months ago) |
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remediator
IllustriousPotentato


Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
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Re: New Orleans soil and fungi.... [Re: falcon]
#5406235 - 03/15/06 10:11 PM (18 years, 2 months ago) |
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Holy shit will that make it through customs? /Hoy shit do you see the other charges? //Holy shit "Euro 430.00 for shipment to the USA."
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falcon


Registered: 04/01/02
Posts: 8,049
Last seen: 3 hours, 36 minutes
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Re: New Orleans soil and fungi.... [Re: remediator]
#5410577 - 03/16/06 10:52 PM (18 years, 2 months ago) |
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I don't know if it would make it through customs. I didn't look at the price, just the one on the front page, holy shit.
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remediator
IllustriousPotentato


Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
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Re: New Orleans soil and fungi.... [Re: remediator]
#5425018 - 03/21/06 12:12 PM (18 years, 2 months ago) |
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Hi all!
I've been hesitant to update, as there's not much going on at the moment. I'm somewhat frustrated at the loss of some fresh spawn to a compost heap via "lack of awareness" So i have sterile jars just sitting around drying up.
I made the P pulmonarius LC from a flesh clone that didn't break up well in the mother jar, and it seems they are not taking... I did a ghetto peroxide clone of some ostreatus from a restaurant straight to grain (since they are just drying up...) and two of the four have gone so far... i shook the other two today. I've never been so anxious to work and had so little to work with.
On a brighter note, the generous folks here at the Shroomery have brightened my prospects with donations, which i hope to start recieving before the week is up. MycoShack has been crazy mad helpful... offering dishes and cultures and other necessities as well as offering to build a flow hood for us!!! Way to go guys, thanks a bunch. I can't wait to get some agar poured. Jeremy Davis has offered a HEPA filter, and his time and services as well, and i hope we can arrange to have him come up from florida to help us with some demonstrations...He does some awesome work himself at this non-profit... Falcon said he would send some cultures of Hypsizugus tessulatus, which is an awesome companion plant. Pocketmulch offered up some P djamor and columbinus... Would like to have Trametes, as it's highly effective at getting some of the real nasties, like dieldrin and aldrin. I am currently researching Trichoderma as a possible remediator, and looking hard at Phanerochaetes chrysosporium, anybody seen that guy?
Sorry i don't have any pretty pics or more exciting news, soon, soon.
We may have a lab space soon, too. The supplies need a house.
Also Tulane university may become involved, if i have anything to say about it... peace to you all, and thanks to everyone who has participate in the discussion. Things are moving forward. i have high hopes that we can grow tons of mushrooms and clean up this place at the same time, it's my dream...
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Darkenshroom
PsychedelicExplorationist


Registered: 02/24/06
Posts: 683
Loc: I don't exist on this pla...
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Re: New Orleans soil and fungi.... [Re: remediator]
#5428932 - 03/22/06 08:05 AM (18 years, 2 months ago) |
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I am glad to hear that things are looking up down there. I am growing more and more interested in your project. In fact it has prompted me to buy Growing Gourmet and Medicinal Mushrooms and Mycelium Running.
I found the thread you had mentioned earlier that you were concerned over linking. Prospective Liquid for LC's in New Orleans Resto Project?
Is that the one? ^_~ I posted in that thread the only questions I would have regarding using that.
I sincerely wish you the best down there, what you are doing is a great thing. Also I agree with the poster who mentioned contacting Stamets, with his newest book and insights into Mushroom Cultivation being related to the very endeavor you are attempting I would not doubt that he would have an interest.
May your endeavor gain steam and be fruitful (quite literally)!
Darken *smiles*
-------------------- ~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~
Edited by Darkenshroom (03/22/06 08:06 AM)
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remediator
IllustriousPotentato


Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
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Re: New Orleans soil and fungi.... [Re: remediator]
#5474038 - 04/03/06 10:11 AM (18 years, 1 month ago) |
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Well, I thought I'd give you guys a little heads up.
Sort of a pre-update update.
Things are moving along, albeit still too slowly. The weather is getting summery and the volunteers i interact with are leaving and being replaced by new faces.
But i have poured some agar.
I have transferred in the last week: Gill tissue of Macrolepiota rachodes from a local compost pile. agar wedges of Hypsizygus tessulatus from Falcon. " " Neolentinus ponderosus " " " " Stropharia Rugoso-annulata " "
And some oysters from a local restaurant.
And there is a space for the Lab reserved for the Bio-Crew at the ArtEgg, which is a local Art Studio borne of a remodelled egg and butter warehouse... I have heard that the space is 20' X 50' with no windows. And ceiling fans? I'm going to check it out today and write up my proposal for the use of the room... In which case I'm going to want to go ahead with the Flow Hood...
I have a 256cfm squirrel fan at my disposal now. I also have the tools and materials aside from the HEPA, which has been offered by Mr. Jeremy Davis of the Shroomery.
So there are some jars on the way. I put some H tessulatus directly into millet quarts, so we'll see how fast i can get some beds going now. Sadly enough, none of the "spent substrate" leads panned out. I haven't had time myself to track down farms nearby...yet.
Uhm. Lemme see, After i check the mail today and visit the artegg, I'll let you guys know how things are going....
peace and thanks to all who have helped with their time and supplies.
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jlocke85
Stranger


Registered: 12/05/05
Posts: 113
Loc: MI
Last seen: 16 years, 8 days
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Re: New Orleans soil and fungi.... [Re: remediator]
#5481710 - 04/05/06 06:27 AM (18 years, 1 month ago) |
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I'm glad to hear things are at least moving along somewhat down there. Have you checked out any of the spawn at the garden? There were two buckets of oyster that were fully colonized, one of which was fruiting. Then there are three cardboard spawn piles, although they could be made thicker so they don't dry out as easily. They seemed to be doing pretty good when I left, but who knows what has happened. Its likely the haven't been watered much since I left. Hopefully some new dedicated volunteers will come through soon and continue to usher the project along.
- Joe
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Jeremy_Davis
Mycelial NetworkAdministrator


Registered: 04/22/05
Posts: 652
Loc: Florida
Last seen: 12 years, 1 month
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Re: New Orleans soil and fungi.... [Re: jlocke85]
#5490860 - 04/07/06 07:31 PM (18 years, 1 month ago) |
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Did you try Quincy Farms in N. Florida, they're outside Talahassee. Here's their website for their spent compost. I bet you can get it by the tractor trailer full;
http://www.quincycompost.com/
Light and Love, JD
-------------------- Jeremy Davis Educational Concerns for Hunger Organization, Inc. Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta
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remediator
IllustriousPotentato


Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
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Re: New Orleans soil and fungi.... [Re: Jeremy_Davis]
#5520842 - 04/16/06 12:18 PM (18 years, 1 month ago) |
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Ahhhh. Easter Sucks....
So, I'm gearing up for some beds now. I went ahead and ordered some cultures, as the donations seemed to not flood in as much as offers...
Thanks to Falcon, i have the King Stropharia and Hypsizygus tessulatus in quantity, at least.
I ended up using two ounce plastic ramekins for agar work, seems to work just fine - never recieved any petris. 
But everything is doing well. I have about 75 quarts of various species, including a Pleurotus that i ghetto-cloned straight to liquid and subsequently successfully got to pin in-vitro.... So those things look good. The room for the lab is there, but i have stalled on jumping on it, simply due to the utter filthyness of the place. I'm not sure i could get it clean and workable without tons of materials and labor. I'm considering just building an enclosure in the space to house the flow hood. Maybe i'll filter the air in the room heavily, and have positive pressure in the whole lab, too. Up to this point the still air box has done me well.
I would like to hear back from Mycoshack and pocketmulch...
Jeremy Davis is waiting for me to call him, I'm sure...waiting on coordination... I would like to drive down sometime and see how you folks are doing things, and maybe pick up the supplies you have offered me, so I'm trying to work that out.
All in all, I'm disappointed in the relatively little I've produced in such a long spread of weeks, but things are definitely at a hot turning point. I've got lots of spawn running here, and the first large project is quickly becoming a reality, so I'm excited.
Maybe I'll snap some shots of the pulmonarius fruiting from the test jar, and some of my ramekin petris to show off, but there's really not much to look at. I plan to document the projects on site relatively thoroughly though, so I'll get some eyecandy going soon enough...
Hey. Thanks to the shroomery I've gotten started at least. Made a great friend locally, Made some contacts that could prove fruitful for a variety of reasons, and I'm learning a lot about myself... Hopefully I'll be eating vegetables from a new orleans garden late next year, and feel safe doing so...
Peace to all of you, and I'm always looking for links to information about specific organisms that have potential applications in bioremediation... chickenofthewoods at yahoo dot com.
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Jeremy_Davis
Mycelial NetworkAdministrator


Registered: 04/22/05
Posts: 652
Loc: Florida
Last seen: 12 years, 1 month
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Re: New Orleans soil and fungi.... [Re: remediator]
#5526171 - 04/17/06 06:15 PM (18 years, 1 month ago) |
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I never got your address, and I'm moving to PA for a new job, so I've been unbelievably busy and swamped by life and all. I apologize. I PM'd you with the specifics, so we can get you up and running before I take off for PA...Baby just turned 2, doing cultivation classes every weekend, I'm working 7 day weeks, and lost or misplaced your address and number!!! I can get my HEPA out of storage this week. I imagine the shipping is gonna be a bit much for the items we'll send so if ya can split it with me it'd be way cool. I'll send it out from the office next week if you promise to keep e-mailing me reminders!!! I also only check the boards once or twice a week right now because of the work load and pressures of packing and moving everything I own all by my lonesome as well as training new volunteers to run the ECHO projects in my stead. I know I need some time management skills! Oh and I'm still working on my degree, so I'm still in classes too. At 28 you'd think I'd be more together, but hey that's life. Never a dull moment! I always marveled at people who have time to be bored. Light and Love, Jeremy Davis
-------------------- Jeremy Davis Educational Concerns for Hunger Organization, Inc. Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta
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