Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineSuntzu
Geek
 User Gallery

Registered: 10/14/99
Posts: 1,396
Last seen: 3 months, 5 days
Re: Smelt Jars for contamination. I was taken to ER!!! [Re: Jammer]
    #539048 - 02/03/02 02:58 AM (21 years, 11 months ago)

Gotta hop in here. . .interesting thread, and I very much hope for a full recovery. I've also [stupidly] smelled questionable growth and gotten light headed and woozy for a few hours afterward...

But there's little chance this is a C. neoformans infection. I'd say no chance. I have some clinical bacteriology lab experience, and every fungal culture we got would first be grown at room temperature. This is, among other things, a safety precaution. Some organisms LIKE C. neoformans only morph into the infectious stage at higher incubation temperatures, namely 37 C [read that, mammalian body temperature]. It's kinda cool actually; it grows mycelium-like at room temperature, and only transforms into its 'yeast-stage' at 37. To identify it, we would take a look at suspect room temp. fuzzy growth. . .if it looked a certain way, we would then very carefully subculture and secure the plate and put it in the 37C+elevated CO2. If it were a dimorphic fungus like neoformans, it wouldn't grow fuzzy, it would be mucoid/wet, like the above pic.

So basically, unless they were incubated much too hot AND you managed to get an environmentally rare yeast into your mix. . .AND are somewhat immunocompromised; The weight of evidence points toward anaphylaxis--which could have been deadly, but likely much sooner after sniffing. Or a secondary source, i.e. you just happen to get sick at the same time. But hey, I don't want to play doctor here, I just know it isn't that easy to accidentally cultivate neoformans.. . shit, I think in 20 years at that lab they grew it three times total.

THIS IS A LINK YOU SHOULD ABSOLUTELY CHECK OUT, the CDC is quite good at identifying suspect contaminants [for a fee] but are also quite available to confer with you on the phone about suspect sniffings:

http://www.cdc.gov/ncidod/dbmd/mdb/labreference.htm

The last paragraph on that page sounds like it might be of use to this forum.

Here's another link to some pretty good CDC info on the beast: for a really good time, do a search on any of the other dimorphic fungal diseases, preferably with pics :P

http://www.cdc.gov/ncidod/eid/vol4no1/buchanan.htm

Growth In Vivo

To cause infection in humans, a C. neoformans isolate must grow at 37oC in an atmosphere of approximately 5% CO2 and at a pH of 7.3 to 7.4. To survive at 37oC, the organism must have an intact gene that encodes the C. neoformans calcineurin A catalytic subunit (10). Calcineurin is a serine-threonine specific phosphatase that is activated by Ca2+-calmodulin and is involved in stress responses in yeasts (10). Although calcineurin A mutant strains of C. neoformans can grow at 24oC, they cannot survive in vitro at 37oC, in 5% CO2, or at alkaline pH (10). Since these are similar to conditions in the host, one would predict that the calcineurin A mutant would not survive in the human host. In support of that prediction, Odom et al. have shown that such mutants are not pathogenic for immunosuppressed rabbits (10). Calcineurin A appears to be a basic requirement for C. neoformans survival in the host and consequently is a necessary factor for the pathogenicity of the organism.



Extras: Unfilter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* Post deleted by MOE THE MAD SCIENTIST
( 1 2 all )
vatoloco 10,087 21 04/30/02 03:27 AM
by Zebedee
* Are my Allen jars contaminated? Angry Mycologist 1,703 6 01/16/03 09:34 AM
by MAIA
* Condensation in jars contamination? gonnadoit 1,515 1 07/01/02 05:20 AM
by DreaMaTrix
* Only jar contaminated!! HELP!! McNugs 549 1 12/29/02 09:59 AM
by Anonymous
* ZAYParinoid: light and contamination Sugaree 2,031 10 03/24/02 05:10 PM
by ParticleMan
* PF jar contams twice (now 3) in a row. upupup 5,816 18 05/30/19 02:12 PM
by Yowtzie
* My jars are ugly looking. FatSean 2,979 10 12/28/01 02:05 AM
by Snobrdr311
* Trichoderma in jars. Joshua 4,063 9 02/19/02 02:30 AM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
42,882 topic views. 26 members, 194 guests and 37 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.012 seconds on 17 queries.