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Offlineoutlawimmortal
the originaloutlaw
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Registered: 11/03/05
Posts: 158
Last seen: 14 years, 25 days
how long would a sterilised PF jar stay sterile for?
    #5327763 - 02/22/06 12:02 PM (17 years, 11 months ago)

i sterilised some 1/2 pint PF jars about 2 months ago. i ordered some from some rip off merchant and the syringe didn't work. now i have a syringe from a sponsor could i inject immediately or should i sterilise again?

i only ask because there is no sign of contamination at all

many thanks


--------------------
...i just got thrown out of a bakery for asking if they sold lambs bread


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OfflineOatman2000
-=Outa Space=-
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Re: how long would a sterilised PF jar stay sterile for? [Re: outlawimmortal]
    #5327800 - 02/22/06 12:13 PM (17 years, 11 months ago)

hmmm.... 2 mths??!?

i would re steralize just to be safe


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Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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InvisibleRoom4Shroom
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Posts: 1,195
Re: how long would a sterilised PF jar stay sterile for? [Re: outlawimmortal]
    #5327811 - 02/22/06 12:15 PM (17 years, 11 months ago)

Good question - I guess it depends on how well they're sealed. If you've kept them in a clean area and have had them wrapped in foil, I think you'll be all right.

You don't want to re-sterilize your jars too many times, because it will screw up the water content inside.
Personally, I would dump them out and make new jars ... especially if you just have one syringe. You can always just make a jar of Liquid Culture and try out the jars. That way you're not really running any risk of running out of spores.


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InvisibleRoadkillM
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Re: how long would a sterilised PF jar stay sterile for? [Re: outlawimmortal]
    #5327852 - 02/22/06 12:24 PM (17 years, 11 months ago)

The problem isn't if you should re-sterilize your jars...

if you don't see any contams...it isn't contaminated.

So there is no need to re-sterilize the jars of substrate!~



The question is...did you lose some of the water content inside the jars?

Where the jars stored in a hot place? or a cool place?

How many jars are we talking about?

Feeling lucky?


:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisibletiny_rabid_birds
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Re: how long would a sterilised PF jar stay sterile for? [Re: Roadkill]
    #5328035 - 02/22/06 01:10 PM (17 years, 11 months ago)

If it's less than like 10 jars, I'd just scrap what's inside and make new substrate. 2 months is a long time for the moisture content to stay right, and it'd be like an hour and a half to make new jars you know to be viable, compared to possibly wasting a spore syringe.


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InvisibleRoom4Shroom
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Re: how long would a sterilised PF jar stay sterile for? [Re: tiny_rabid_birds]
    #5328054 - 02/22/06 01:17 PM (17 years, 11 months ago)

Quote:

tiny_rabid_birds said:
If it's less than like 10 jars, I'd just scrap what's inside and make new substrate.  2 months is a long time for the moisture content to stay right, and it'd be like an hour and a half to make new jars you know to be viable, compared to possibly wasting a spore syringe.





:thumbup: :thumbup:


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Offlinehyphae
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Re: how long would a sterilised PF jar stay sterile for? [Re: Room4Shroom]
    #5328994 - 02/22/06 06:50 PM (17 years, 11 months ago)

It all depends on initial moisture content and the temp it's been stored at. They may just be perfectly fine as Road said feeling lucky?


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Getting the most out of your casings!, A pinning strategy.
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Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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