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outlawimmortal
the originaloutlaw

Registered: 11/03/05
Posts: 158
Last seen: 14 years, 25 days
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how long would a sterilised PF jar stay sterile for?
#5327763 - 02/22/06 12:02 PM (17 years, 11 months ago) |
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i sterilised some 1/2 pint PF jars about 2 months ago. i ordered some from some rip off merchant and the syringe didn't work. now i have a syringe from a sponsor could i inject immediately or should i sterilise again?
i only ask because there is no sign of contamination at all
many thanks
-------------------- ...i just got thrown out of a bakery for asking if they sold lambs bread
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: how long would a sterilised PF jar stay sterile for? [Re: outlawimmortal]
#5327800 - 02/22/06 12:13 PM (17 years, 11 months ago) |
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hmmm.... 2 mths??!?
i would re steralize just to be safe
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Room4Shroom


Registered: 01/15/03
Posts: 1,195
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Re: how long would a sterilised PF jar stay sterile for? [Re: outlawimmortal]
#5327811 - 02/22/06 12:15 PM (17 years, 11 months ago) |
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Good question - I guess it depends on how well they're sealed. If you've kept them in a clean area and have had them wrapped in foil, I think you'll be all right.
You don't want to re-sterilize your jars too many times, because it will screw up the water content inside. Personally, I would dump them out and make new jars ... especially if you just have one syringe. You can always just make a jar of Liquid Culture and try out the jars. That way you're not really running any risk of running out of spores.
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: how long would a sterilised PF jar stay sterile for? [Re: outlawimmortal]
#5327852 - 02/22/06 12:24 PM (17 years, 11 months ago) |
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The problem isn't if you should re-sterilize your jars...
if you don't see any contams...it isn't contaminated.
So there is no need to re-sterilize the jars of substrate!~
The question is...did you lose some of the water content inside the jars?
Where the jars stored in a hot place? or a cool place?
How many jars are we talking about?
Feeling lucky?
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Re: how long would a sterilised PF jar stay sterile for? [Re: Roadkill]
#5328035 - 02/22/06 01:10 PM (17 years, 11 months ago) |
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If it's less than like 10 jars, I'd just scrap what's inside and make new substrate. 2 months is a long time for the moisture content to stay right, and it'd be like an hour and a half to make new jars you know to be viable, compared to possibly wasting a spore syringe.
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Room4Shroom


Registered: 01/15/03
Posts: 1,195
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Re: how long would a sterilised PF jar stay sterile for? [Re: tiny_rabid_birds]
#5328054 - 02/22/06 01:17 PM (17 years, 11 months ago) |
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Quote:
tiny_rabid_birds said: If it's less than like 10 jars, I'd just scrap what's inside and make new substrate. 2 months is a long time for the moisture content to stay right, and it'd be like an hour and a half to make new jars you know to be viable, compared to possibly wasting a spore syringe.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: how long would a sterilised PF jar stay sterile for? [Re: Room4Shroom]
#5328994 - 02/22/06 06:50 PM (17 years, 11 months ago) |
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It all depends on initial moisture content and the temp it's been stored at. They may just be perfectly fine as Road said feeling lucky?
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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