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OfflineMisterMyco
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Preparing Grain Jars to be "super-inoculated"
    #5324601 - 02/21/06 04:10 PM (17 years, 11 months ago)

Most people who have grown longer than a few months have realized the benefits of using liquid culture.  Turning one drop of spore solution into 60mL of living, healthy mycelia is a rewarding experience.  I've always liked injecting as much growth material (spores, LC) as possible into my grain jars and I developed a way to allow me to do so.  I believe that the more mycelia injected, the quicker the jar grows out to completion and the quicker that you can move onto the next step. 

To facilitate the injection of 5-7mL of culture solution, you have to ensure that your grains are a little bit drier than standard or you'll end up with standing water in the bottom.  I add an amount of dry vermiculite to each jar before I put the tyvek and the lid on and I shake the jar well.  This dry vermiculite lowers the overall moisture percentage for the jar and allows you to inject 5+mL of culture solution without worrying about excess water causing contamination or slowing mycelial growth.

To find out how much vermiculite to use, determine how much solution you'd like to add.  I find that about 8mL of a THICK culture solution leads to full colonization in about 5 days.  So I put 8mL of water in the bottom of a shot glass and added vermiculite until it had the consistency of a casing layer or other substrate.  That amount is the amount that I use in my jars, knowing that the excess vermiculite will absorb the solution and get the jars off to an explosive start.
Hope this helps :smile:


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"I have never, in all my life, not for one moment, been tempted toward religion of any kind. The fact is that I feel no spiritual void. I have my philosophy of life, which does not include any aspect of the supernatural."
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OfflineSnaggletooth
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Re: Preparing Grain Jars to be "super-inoculated" [Re: MisterMyco]
    #5324622 - 02/21/06 04:18 PM (17 years, 11 months ago)

Interesting, I'll have to remember this.......... :tongue2:


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Atheist Chat


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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Re: Preparing Grain Jars to be "super-inoculated" [Re: MisterMyco]
    #5324716 - 02/21/06 04:41 PM (17 years, 11 months ago)

I just boil the grain till it expands 50 percent. Say a pot filled with 3 inches of grain should be cooked simmered till it expands up to six inches in the pot. Drain, jar up, cook, cool, nock up.

Simple yet very effective. Dont let the kernels explode. Thats the key.

popped kernels = to much water content = soggy after lc inoculation


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OfflineMisterMyco
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Re: Preparing Grain Jars to be "super-inoculated" [Re: Corporal Kielbasa]
    #5324769 - 02/21/06 04:52 PM (17 years, 11 months ago)

But I like to inject a LOT of solution, thats the whole point here.


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"I have never, in all my life, not for one moment, been tempted toward religion of any kind. The fact is that I feel no spiritual void. I have my philosophy of life, which does not include any aspect of the supernatural."
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InvisibleCorporal Kielbasa

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Re: Preparing Grain Jars to be "super-inoculated" [Re: MisterMyco]
    #5324807 - 02/21/06 05:02 PM (17 years, 11 months ago)

I use any thing above 5 cc's more around 8 or 9 sometimes more. Im not knocking or anything like that just adding to the conversation.

8 cc's then a shake then a shake 4 days later. All done within 2 weeks.

I just like to do as few steps as i can in the process. So i dont waste or take up more time out of my day.


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OfflineHypercube
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Re: Preparing Grain Jars to be "super-inoculated" [Re: MisterMyco]
    #5324808 - 02/21/06 05:02 PM (17 years, 11 months ago)

Great tip, simple and effective.

I find WBS colonizes quicker with verm in the jars anyways, than without. I'll be doing this on my next run.

5 boomers fe'dis ma'fugga!


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OfflineMisterMyco
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Re: Preparing Grain Jars to be "super-inoculated" [Re: Corporal Kielbasa]
    #5324816 - 02/21/06 05:03 PM (17 years, 11 months ago)

TWO WEEKS? wow, are you talking about spores or LC? I hardly ever use spores on anything but agar. This is a four to five day thing to full colonization...

HyperCube - I definatly thing that having vermiculite in the jars leads to more rhizomorphic and rapid growth. Keep me posted on what you find


--------------------
"I have never, in all my life, not for one moment, been tempted toward religion of any kind. The fact is that I feel no spiritual void. I have my philosophy of life, which does not include any aspect of the supernatural."
Isaac Asimov


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InvisibleCorporal Kielbasa

Registered: 05/29/04
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Re: Preparing Grain Jars to be "super-inoculated" [Re: MisterMyco]
    #5324821 - 02/21/06 05:05 PM (17 years, 11 months ago)

Lc but lower then normal incubation temps. I said WITHIN two weeks


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OfflinerDr4g0n
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Re: Preparing Grain Jars to be "super-inoculated" [Re: Corporal Kielbasa]
    #5325294 - 02/21/06 06:46 PM (17 years, 11 months ago)

i did 3cc with my lc and was ready to go after 5 days and there was no excess of water in the jar, but if i decide to use more than 3cc, ill be sure to toss in some dry verm; thats a good idea.


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i can speel... im just too lazy to sppelcheck.

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InvisibleCorporal Kielbasa

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Re: Preparing Grain Jars to be "super-inoculated" [Re: MisterMyco]
    #5325302 - 02/21/06 06:47 PM (17 years, 11 months ago)

my bad i forgot to mention its with 4 quart spawn bags oops


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OfflineMisterMyco
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Re: Preparing Grain Jars to be "super-inoculated" [Re: Corporal Kielbasa]
    #5325361 - 02/21/06 06:57 PM (17 years, 11 months ago)

Oh, heh, that changes everything, thats pretty good :wink:


--------------------
"I have never, in all my life, not for one moment, been tempted toward religion of any kind. The fact is that I feel no spiritual void. I have my philosophy of life, which does not include any aspect of the supernatural."
Isaac Asimov


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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Re: Preparing Grain Jars to be "super-inoculated" [Re: MisterMyco]
    #5325370 - 02/21/06 06:59 PM (17 years, 11 months ago)

I was wondering why it wasnt sounding right  :chef:


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Offlineblackout
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Re: Preparing Grain Jars to be "super-inoculated" [Re: Corporal Kielbasa]
    #5327157 - 02/22/06 04:52 AM (17 years, 11 months ago)

It is much easier if you add precise amount of water to jars. If you click on microwave growlog in my sig you can see very rapidly colonising jars, after only 3 days they have lots of growth.


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OfflineOatman2000
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Re: Preparing Grain Jars to be "super-inoculated" [Re: blackout]
    #5327404 - 02/22/06 09:01 AM (17 years, 11 months ago)

i add verm to my jars also....

but ther are 2 ways i make jars..
Steep grains... then add verm after drip dry. then load up, and pc.
(this helps out so you don't haffta wait 30 min to drip dry. the verm soaks up moisture)

#2 as stamets states, load up dry grain, and add water = to grain line, then pc this. I'm not sure of a way to add verm to this mixture?? maybee after pc, but then you risk contams.


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Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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Offlineblackout
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Re: Preparing Grain Jars to be "super-inoculated" [Re: Oatman2000]
    #5327676 - 02/22/06 11:25 AM (17 years, 11 months ago)

Never saw that stamets recommendation, in TMC the water is just above the grains, I have heard this recommended before. If adding the verm I would simply sprinle on top of the grain and water and not shake until after


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OfflineOatman2000
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Re: Preparing Grain Jars to be "super-inoculated" [Re: blackout]
    #5327741 - 02/22/06 11:54 AM (17 years, 11 months ago)

Quote:

blackout said:
Never saw that stamets recommendation, in TMC the water is just above the grains, I have heard this recommended before. If adding the verm I would simply sprinle on top of the grain and water and not shake until after




Page 45 of TMC
"Preparation of Grain Spawn"


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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