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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
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GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?)
#5325249 - 02/21/06 06:38 PM (17 years, 11 months ago) |
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 ok so i built a glove box against my own wishes. then i re-built it. then i obsessed about it some more,
then i tried using it...
i figured i would use it to perform a simple task that doesn't necessarily require a GB. just so i could get some experience using it... ie, aspirating LC from baby bottles into vacutainers via sterile/wrapped syringes...
i abandoned ship after realizing that after spraying the oust, it would not dry out so i could work. i had cleaned all the surfaces w/ 90% iso, and let it mostly dry out. i tried spraying the oust in the GB closed (lid on)- no avail. then i dried it all out and cleaned it all (not-as thoroughly this time) w/ 90% iso then sprayed the oust in the air surrounding the GB and into the GB, then closed the lid. neither way would dry out- and i waited too. i mean, drank a beer and watched Aqua Teen waited
i have no fan, i have no ventilation-
other than the lid is the original rubbermaid lid w/ no extra gasket/seal/plastic sheet. so there is minimal AE through the lid
i've read thru several GB ventilation threads and I KNOW this comes up ALOT (so, i'm sorry)
but why is something that should be SO FUCKING EASY make itself so hard?
i was against the idea of a fan i know some old hands use various types of vents i'm new to advanced grow shit, especially teks using methods i strayed away from - ie, glove box use... now i dont see HOW you can spray shit down and have it DRY w/o a fan. i DON'T know of any task that would require a fan during actual work... 
fire away...
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
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Holydiver
Stranger



Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: krill]
#5325259 - 02/21/06 06:40 PM (17 years, 11 months ago) |
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The single most effective glovebox I've had over the years is a plain old STILL AIR one. Simplify.
-------------------- To find a place to live between the negatives and positives.
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rDr4g0n
Young Hand

Registered: 01/17/06
Posts: 587
Last seen: 17 years, 7 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: krill]
#5325269 - 02/21/06 06:41 PM (17 years, 11 months ago) |
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perhaps the alcohol is enough to clean it without the oust? a posi-flow gb always made more since to me anyways just as long and the intake has a good filter (hepa?).
-------------------- i can speel... im just too lazy to sppelcheck. My first trip (good read) - Speed Leaching Poo! - My Second Trip (with art)
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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: rDr4g0n]
#5325289 - 02/21/06 06:45 PM (17 years, 11 months ago) |
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Quote:
just as long and the intake has a good filter (hepa?).
care to elaborate?
hey Holydiver...
do you have a BIG GB? i used the 4" dryer ducting w/ thise SAME EXACT gloves the first time i built mine. then i found some elbow length gloves and used 6" hose clamps to affix em to my arm ports. (thanks HippyChick!) those big arm things were too much for my rubbermaid...
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
Edited by krill (02/21/06 06:48 PM)
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Holydiver
Stranger



Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: krill]
#5325315 - 02/21/06 06:49 PM (17 years, 11 months ago) |
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I don't know the size, but it can comfortably hold 10 quart jars to do G2G work and so on. I went to still air after realizing that trying to attach fans and filters was introducing more mold spores than it was worth, and never looked back.
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HippieChick
Chicks can do it too!


Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 13 days
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) *DELETED* [Re: krill]
#5325322 - 02/21/06 06:50 PM (17 years, 11 months ago) |
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Post deleted by HippieChickReason for deletion: .
-------------------- Peace,Love and Happiness HC Freedoms just another word for nothing left to lose.............. I LUV My Greenhouse http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848 My First Pans http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: rDr4g0n]
#5325345 - 02/21/06 06:54 PM (17 years, 11 months ago) |
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You may indeed be spraying too much Oust but being a little wet inside won't hurt a thing just do your thing. Oust is just for scrubbing the air anyway, I use clorox wipes for wiping down the inside of the GB and anything inside of it. Just use alcohol swabs to sterilize the needle, it should work just fine.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: HippieChick]
#5325348 - 02/21/06 06:54 PM (17 years, 11 months ago) |
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^^^I have one like that, I just went that route....
--------------------
Atheist Chat
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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: HippieChick]
#5325352 - 02/21/06 06:55 PM (17 years, 11 months ago) |
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so, Hippie Chick... lemme ask ya, do you run the fan while working? and exactly what type of filter did you use / is acceptable?
and, that GB is fecking awesome! definitely a woman's touch there... notice the suction-cup,soap-dish-thingy guys?
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
Edited by krill (02/21/06 06:56 PM)
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: krill]
#5327421 - 02/22/06 09:13 AM (17 years, 11 months ago) |
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i built a GB exactly like yours HC.
but i ended up converting my posi flow into a still air.
(covering up the outtake hole.)
when i spray, i turn my fan on while i wait. then turn it off to work. it still has the hepa filter on it, but no way to load jars into it.
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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rDr4g0n
Young Hand

Registered: 01/17/06
Posts: 587
Last seen: 17 years, 7 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Oatman2000]
#5327450 - 02/22/06 09:33 AM (17 years, 11 months ago) |
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when i build mine, it will be just like hippie chicks. posi flow with hepa. you open the top, clean with alcohol, load up what your gonna work with, close the top, turn on teh fan. let that run for like 30 min to get nice clean air in there.
then when your ready to work, you can either leave the fan running, or just turn it off. either way, i think itll be clean and sterile for a good while so you can get whatever you need to do done.
a regular still air would be just as good, but you dont get the added benefit of sucking in clean air. i mean you can spray oust and (hopefully) disinfect the standing air, but i think the extra cash to set up posi-flow would be worth it.
also i should point out that ive used neither glove boxes, but i plan to build one soon... so this isnt from experience, just from logical deduction.
-------------------- i can speel... im just too lazy to sppelcheck. My first trip (good read) - Speed Leaching Poo! - My Second Trip (with art)
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: rDr4g0n]
#5327494 - 02/22/06 09:59 AM (17 years, 11 months ago) |
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yes but with HC's she has a big hole in the other side of the hepa filter for (the posi flow) gotta have an output.
it's big enough that you can fit jars in and out while working.
but you MUST work with the fan on.
IME i find that i drain lantern batteries too quickly for the use, and if doing a g/g i find that the sudden BLAST of frfesh air halts the growth.
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Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Cubenisseur
Mad Props


Registered: 12/04/05
Posts: 1,392
Loc: Indian Land
Last seen: 13 years, 10 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Oatman2000]
#5327536 - 02/22/06 10:24 AM (17 years, 11 months ago) |
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Question for Hippie Chick, How long does a battery that size last while running a fan like that?
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Cubenisseur]
#5327664 - 02/22/06 11:19 AM (17 years, 11 months ago) |
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A fan is the worst possible thing you can do to a glovebox. There is also no need to spray lysol or oust in a GB. Wipe it with a damp cloth and go to work. There is nothing sterile about a glovebox. STILL air is what you want. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: krill]
#5327675 - 02/22/06 11:25 AM (17 years, 11 months ago) |
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-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: RogerRabbit]
#5327697 - 02/22/06 11:35 AM (17 years, 11 months ago) |
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I agree still air allows no movement I still scrub the air before closing (added insurance). Those posi-flows do work just fine for many but those filters do allow usually 3% contams to pass but from what I've seen this has little effect on contams rate, I suppose those who have high spore loads in the environment may not have the same results and still air would be the better choice. I use still air GB's exclusively and have never had a problem and they're much cheaper to construct but hell constructing and experimenting is half the fun with this hobby right!
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: hyphae]
#5327754 - 02/22/06 11:59 AM (17 years, 11 months ago) |
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that's why i converted mine to still air...
Road: you don't use gloves? I hate using those damn gloves. i think i am gonna chop off the hand portion and leave the arm pieces.
so when i stick my arms in it will produce a seal. i have skinny arms.
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
Edited by oatman2000 (02/22/06 11:59 AM)
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Oatman2000]
#5327784 - 02/22/06 12:10 PM (17 years, 11 months ago) |
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I tried gloves and they were too cumbersome.
so I just screw on the cap peice when my arms aren't in the box.
I have large fore arms...so I get a good seal between my arms and the flange.

tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Roadkill]
#5327851 - 02/22/06 12:24 PM (17 years, 11 months ago) |
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nice... i think i am going to do this...
i am friekin retarded when it comes to working with those gloves on!
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Hotnuts
old hand


Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 25 days, 18 hours
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: hyphae]
#5328040 - 02/22/06 01:12 PM (17 years, 11 months ago) |
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Quote:
hyphae said: I agree still air allows no movement I still scrub the air before closing (added insurance). Those posi-flows do work just fine for many but those filters do allow usually 3% contams to pass but from what I've seen this has little effect on contams rate, I suppose those who have high spore loads in the environment may not have the same results and still air would be the better choice. I use still air GB's exclusively and have never had a problem and they're much cheaper to construct but hell constructing and experimenting is half the fun with this hobby right!
Hyphae. With your box; the exhaust hose is mounted in that fassion to allow you freedom with glooved hands? Or, are the glooves mounted to the arm holes as well? I'm guessing they're not mounted.... 
That's a great idea and easily solves my problem with mounted glooves that are tight fit enough to handle tools exc. Then getting out of the glooves after work..lol! If I mounted the glooves I like to use, it would take me an hour to get out of them...
Edited by hotnutz (02/22/06 01:19 PM)
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: krill]
#5328602 - 02/22/06 04:44 PM (17 years, 11 months ago) |
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I use & prefer a tight STILL AIR G/D.
Once built, I realized that anything moist inside caused minor
CONDENSATION , that would not evaporate out. 
To alleviate the problem.
I simply cut a 7.5 inch square hole in one side, then installed a 8X8 inch square panel of TYVEK tightly over it, which allows for ambient gas exchange (</>). While still maintaining the integrity of a sterile interior chamber.
After some use of that one, I built a much bigger G/B. (for more interior visibility, space, comfort & ease of use).
On the larger G/B. On the side, I installed a 12X12 inch tight panel of hepa/ulpa grade filter material.
Works PERFECTLY.
No fan, no nada, just STILL AIR.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Hotnuts]
#5328761 - 02/22/06 05:40 PM (17 years, 11 months ago) |
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Quote:
hotnutz said:
Quote:
hyphae said: I agree still air allows no movement I still scrub the air before closing (added insurance). Those posi-flows do work just fine for many but those filters do allow usually 3% contams to pass but from what I've seen this has little effect on contams rate, I suppose those who have high spore loads in the environment may not have the same results and still air would be the better choice. I use still air GB's exclusively and have never had a problem and they're much cheaper to construct but hell constructing and experimenting is half the fun with this hobby right!
Hyphae. With your box; the exhaust hose is mounted in that fassion to allow you freedom with glooved hands? Or, are the glooves mounted to the arm holes as well? I'm guessing they're not mounted.... 
That's a great idea and easily solves my problem with mounted glooves that are tight fit enough to handle tools exc. Then getting out of the glooves after work..lol! If I mounted the glooves I like to use, it would take me an hour to get out of them...
My GB's have only arm holes. My Big one has flex hose that are not attached to the gloves and works like a charm. My smaller GB I just used gloved hands to do my shit no problems there either. The GB's are sealed so no air enters the arm holes because of static pressure. Extremely simple in design and effectiveness.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Drone
Derka Derka Derka


Registered: 07/22/04
Posts: 667
Loc: Maryland
Last seen: 5 years, 1 month
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: hyphae]
#5328953 - 02/22/06 06:34 PM (17 years, 11 months ago) |
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wow, you guys are making this way to hard. I have a still air glovebox.
All I do is rub everything down with a bleach/water solution and put it in the glovebox(including a bleach/water spray bottle). then i spray everything down inside the GB after it is closed....like the walls, rub down the jars or whatever your using. Let it set for 10 mins or so and then go to work. Let it be wet inside, it dosnt matter...
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Hotnuts
old hand


Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 25 days, 18 hours
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Drone]
#5331157 - 02/23/06 10:41 AM (17 years, 11 months ago) |
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I'm aware of how to use a gloove box. I have a filtered box that's been causing me grief as of late and now i'm building a still air box out of a large aquarium. I like the dryer exhaust hose deal in Hyphae's box. I do agar work and it makes me paranoid a bit with the still air box having open arm holes. But with Hyphae's rig, that should work out nicely for agar work and comfortability while working...
Edited by hotnutz (02/23/06 10:42 AM)
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Hotnuts]
#5331258 - 02/23/06 11:10 AM (17 years, 11 months ago) |
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Hyphae's rig:
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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