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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: krill]
#5328602 - 02/22/06 04:44 PM (17 years, 11 months ago) |
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I use & prefer a tight STILL AIR G/D.
Once built, I realized that anything moist inside caused minor
CONDENSATION , that would not evaporate out. 
To alleviate the problem.
I simply cut a 7.5 inch square hole in one side, then installed a 8X8 inch square panel of TYVEK tightly over it, which allows for ambient gas exchange (</>). While still maintaining the integrity of a sterile interior chamber.
After some use of that one, I built a much bigger G/B. (for more interior visibility, space, comfort & ease of use).
On the larger G/B. On the side, I installed a 12X12 inch tight panel of hepa/ulpa grade filter material.
Works PERFECTLY.
No fan, no nada, just STILL AIR.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Hotnuts]
#5328761 - 02/22/06 05:40 PM (17 years, 11 months ago) |
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Quote:
hotnutz said:
Quote:
hyphae said: I agree still air allows no movement I still scrub the air before closing (added insurance). Those posi-flows do work just fine for many but those filters do allow usually 3% contams to pass but from what I've seen this has little effect on contams rate, I suppose those who have high spore loads in the environment may not have the same results and still air would be the better choice. I use still air GB's exclusively and have never had a problem and they're much cheaper to construct but hell constructing and experimenting is half the fun with this hobby right!
Hyphae. With your box; the exhaust hose is mounted in that fassion to allow you freedom with glooved hands? Or, are the glooves mounted to the arm holes as well? I'm guessing they're not mounted.... 
That's a great idea and easily solves my problem with mounted glooves that are tight fit enough to handle tools exc. Then getting out of the glooves after work..lol! If I mounted the glooves I like to use, it would take me an hour to get out of them...
My GB's have only arm holes. My Big one has flex hose that are not attached to the gloves and works like a charm. My smaller GB I just used gloved hands to do my shit no problems there either. The GB's are sealed so no air enters the arm holes because of static pressure. Extremely simple in design and effectiveness.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Drone
Derka Derka Derka


Registered: 07/22/04
Posts: 667
Loc: Maryland
Last seen: 5 years, 1 month
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: hyphae]
#5328953 - 02/22/06 06:34 PM (17 years, 11 months ago) |
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wow, you guys are making this way to hard. I have a still air glovebox.
All I do is rub everything down with a bleach/water solution and put it in the glovebox(including a bleach/water spray bottle). then i spray everything down inside the GB after it is closed....like the walls, rub down the jars or whatever your using. Let it set for 10 mins or so and then go to work. Let it be wet inside, it dosnt matter...
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Hotnuts
old hand


Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 25 days, 18 hours
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Drone]
#5331157 - 02/23/06 10:41 AM (17 years, 11 months ago) |
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I'm aware of how to use a gloove box. I have a filtered box that's been causing me grief as of late and now i'm building a still air box out of a large aquarium. I like the dryer exhaust hose deal in Hyphae's box. I do agar work and it makes me paranoid a bit with the still air box having open arm holes. But with Hyphae's rig, that should work out nicely for agar work and comfortability while working...
Edited by hotnutz (02/23/06 10:42 AM)
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: GloveBox standoff: PosiFlow Fan vs. Still Air (FAE?) [Re: Hotnuts]
#5331258 - 02/23/06 11:10 AM (17 years, 11 months ago) |
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Hyphae's rig:
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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