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Shimen
NotGuilty


Registered: 02/10/06
Posts: 180
Loc: Indiana
Last seen: 15 years, 6 months
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Um Why arnt my jars colonizing
#5324375 - 02/21/06 02:52 PM (17 years, 11 months ago) |
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Alright so i know there is not a direct answer for this but i was just wondering if you guys have any suggestions to make me feel better. I am using regular PF jars (BRF) its been almost 4 days now and no sign. They are in a small incubator that i made around 82-85 degrees. Am i doing anything wrong here. Put 1.2 CC of spore solution into each jar. Any suggestions appreciated. Thanks
-------------------- Death is when you become bored with life.
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c_glidden
Stranger
Registered: 12/24/05
Posts: 243
Last seen: 13 years, 2 months
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Re: Um Why arnt my jars colonizing [Re: Shimen]
#5324384 - 02/21/06 02:55 PM (17 years, 11 months ago) |
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wait 2 more weeks, then start worrying
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Razman
Stranger

Registered: 07/07/04
Posts: 514
Last seen: 8 years, 11 months
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Re: Um Why arnt my jars colonizing [Re: Shimen]
#5324385 - 02/21/06 02:55 PM (17 years, 11 months ago) |
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wait longer.. spores arent as quick as liquid culture..
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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Re: Um Why arnt my jars colonizing [Re: Shimen]
#5324387 - 02/21/06 02:55 PM (17 years, 11 months ago) |
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LOL! 4 days?
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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Shimen
NotGuilty


Registered: 02/10/06
Posts: 180
Loc: Indiana
Last seen: 15 years, 6 months
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Re: Um Why arnt my jars colonizing [Re: Delinquentes]
#5324391 - 02/21/06 02:57 PM (17 years, 11 months ago) |
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I thought they were only susposed to take 2-5 days. Owell guess i was misinformed. I guess i will continue to wait makes me feel a bit better though thanks
-------------------- Death is when you become bored with life.
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DIRTYMAN
Jesusdon'tcomethrough thecotton.

Registered: 11/18/05
Posts: 18,558
Loc: CZ NIGGUH
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Re: Um Why arnt my jars colonizing [Re: Shimen]
#5324393 - 02/21/06 02:57 PM (17 years, 11 months ago) |
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My jars took about a week to show any growth, and that was with a grain to grain transfer. Give it time, don't check your shit everyday, and relax!
-------------------- I'm racist. http://k-k-k.com/
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Shimen
NotGuilty


Registered: 02/10/06
Posts: 180
Loc: Indiana
Last seen: 15 years, 6 months
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Re: Um Why arnt my jars colonizing [Re: DIRTYMAN]
#5324394 - 02/21/06 02:58 PM (17 years, 11 months ago) |
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Is it bad to check them everyday??
-------------------- Death is when you become bored with life.
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c_glidden
Stranger
Registered: 12/24/05
Posts: 243
Last seen: 13 years, 2 months
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Re: Um Why arnt my jars colonizing [Re: Shimen]
#5324402 - 02/21/06 03:00 PM (17 years, 11 months ago) |
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the more times you open your incubator, the more chance for contams to get in.. exposure to light (although not at the stage you are at) can cause premature pinning which can lead to rotting... everyone is gonna tell you that patience is key
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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Re: Um Why arnt my jars colonizing [Re: c_glidden]
#5324419 - 02/21/06 03:05 PM (17 years, 11 months ago) |
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I have to confess...I inocculated on Sunday and one jar is showing white spots already, but you have to look closely.
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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Shimen
NotGuilty


Registered: 02/10/06
Posts: 180
Loc: Indiana
Last seen: 15 years, 6 months
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Re: Um Why arnt my jars colonizing [Re: Delinquentes]
#5324428 - 02/21/06 03:07 PM (17 years, 11 months ago) |
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Ok well its a little hard to distingish between the flour and the spots i would thinks. I got some very very very small spots that are lighter than the flour. But i just thought it was the flour.
-------------------- Death is when you become bored with life.
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civicracerx7


Registered: 12/30/05
Posts: 397
Last seen: 7 years, 2 months
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Re: Um Why arnt my jars colonizing [Re: Shimen]
#5324741 - 02/21/06 04:46 PM (17 years, 11 months ago) |
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mine had big spots in 2 days
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Um Why arnt my jars colonizing [Re: civicracerx7]
#5324803 - 02/21/06 05:01 PM (17 years, 11 months ago) |
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I recommend taking off the tape as soon as you see any growth. That is if ya used it. Spores can take up to 14-21 days to germinate BTW
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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