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Offlinehookem
Stranger
Registered: 02/21/06
Posts: 8
Last seen: 17 years, 7 months
screwed up last time, trying again
    #5322654 - 02/21/06 12:20 AM (17 years, 11 months ago)

Ok, so this is my first post here, but I've been lurking for a while trying to learn as much as possible.
I thought I knew what I was doing when we bought our spores from spores101, but one or more things went wrong on my first try.
I followed the brown rice flour tek, made my own brown rice. I dont have a PC, so I used steam to sterilize. That was my first mistake.
I waited overnight, atleast... I don't remember if they sat maybe a day or two before I inoculated them.
The second mistake I made was that after inoculation, I didnt replace the tin foil. This was just a misunderstanding... I mustve missed the part that said to put it back.
So after inoculation I put the jars in a cabinet in my friends kitchen that had been sterilized with bleach. We then taped the door shut and agreed that only I was allowed to open it.
About a week into the process there's nothing going on, so I decide that it needs to be warmer, as his apartment keeps in the low 70s. I am completely broke, to the last penny, so I could not afford to build a TiT. Instead, I put my friend's aquarium heater in a tupperware full of water and put it with the jars. This was another mistake. I didnt think the water would evaporate, but it did.
Since the holes in my jars were uncovered, the dry vermiculite at the top got wet, and the majority of my jars contaminated before I realized I did something wrong.
So, that is an overview of what I did wrong, but I am puzzled by one thing. Two of the jars have not become contaminated, and also show no mycelial growth whatsoever. None of the contaminated jars did either. I shook the syringes before inoculation as is instructed. What could be the problem? This is what I want to tackle before Wednesday when I plan on starting the second batch.
The only other thing I can think of is that somewhere I got incorrect ratios and I think I used a small amount too much vermiculite, but the flour is still very apparent in the mix, so I didnt think the vermiculite was too overpowering. Could this be my problem?
Sorry for rambling, but I'm just trying to provide all of you with the most information I can so that hopefully someone can give me some sort of advice.
Any ideas?

Oh sorry I almost forgot, I was using B+ strain from shrooms101.


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OfflineHippieChick
Chicks can do it too!
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Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 13 days
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Re: screwed up last time, trying again *DELETED* [Re: hookem]
    #5322675 - 02/21/06 12:29 AM (17 years, 11 months ago)

Post deleted by HippieChick

Reason for deletion: .



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Offlinehookem
Stranger
Registered: 02/21/06
Posts: 8
Last seen: 17 years, 7 months
Re: screwed up last time, trying again [Re: HippieChick]
    #5322682 - 02/21/06 12:31 AM (17 years, 11 months ago)

I'm planning on making a TiT set up when I finally get paid on Wednesday. Thats actually the only reason that theres been such a delay before trying again.


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Offlinecamelsmoker
smoke up
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Registered: 02/20/05
Posts: 1,310
Loc: The Dirty Dirty
Last seen: 15 years, 1 month
Re: screwed up last time, trying again [Re: hookem]
    #5322684 - 02/21/06 12:32 AM (17 years, 11 months ago)

if i were you i would order grow bags jars are just a waist of time in my opionion due to the fact they never worked well for me and i just got a myco bag with verm and bird seed and it colonized very well you can get them from 10 -14 dollars through vendors on the site take a look around they work well and be carefull letting all that water evaporate with them fishtank heaters you could burn the place down would hate to see a fellow shroom advocate homeless


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No harm can come from questioning. "Best" case scenerio: You prove it right without relying on the fact that it is widely accepted. Argument strengthened. "Worst" case scenerio: You realize you might have been wrong. The new answer might or might not be what you want to hear, but it beats defending yourself with bullshit.
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OfflinemrTtheH
Micro Bio

Registered: 04/01/04
Posts: 188
Loc: C A N A D A
Last seen: 15 years, 4 months
Re: screwed up last time, trying again [Re: hookem]
    #5322691 - 02/21/06 12:37 AM (17 years, 11 months ago)

ive found a cheap way to get heat from a heatingpad on lowsetting by putting it in the large plastic bin that had the jars in it and pop a thermometre in there close the lid and turning it on and off or on higher with a little effort you keep a good temp.


--------------------
It is the mark of an educated mind to be able to entertain a thought without accepting it.
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"Human beings, the potentially highest form of life expression on this planet have built the vast pharmaceutical industry for the central purpose of poisoning the lowest form of life on the planet--germs!"--Dr. Richard Murray


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Offlinehookem
Stranger
Registered: 02/21/06
Posts: 8
Last seen: 17 years, 7 months
Re: screwed up last time, trying again [Re: mrTtheH]
    #5323404 - 02/21/06 10:02 AM (17 years, 11 months ago)

So do you all think that low temps are what is keeping my spores from germinating?


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