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Offlineke1n
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Registered: 11/15/05
Posts: 359
Last seen: 14 years, 6 months
3rd Attempt @ WBS jars - CONTAMED?? [pic]
    #5314187 - 02/18/06 06:19 PM (17 years, 11 months ago)

hey guy this is my 3rd attempt at WBS. Each time I have run into bacteria mold appearing in my jars, first appearing as fast colonizing mycelium. I can clearly see some mycelium and some parts look not so mycelium-like.

This time I soaked my WBS for 24 hours, PCed for 2.5 @16 psi.

I flame sterilized my syringes and dipped the needles in alcohol before injecting. I used LC but the LC is perfeclty clear so I dont think it is contamed.

one of my jars 3-5 days after innoculating:



Is this a contam or is it hard ottell at this point??


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OfflineThai_connection
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Registered: 10/01/05
Posts: 247
Loc: Thailand
Last seen: 13 years, 3 months
Re: 3rd Attempt @ WBS jars - CONTAMED?? [pic] [Re: ke1n]
    #5314474 - 02/18/06 08:07 PM (17 years, 11 months ago)

rinse your wbs often while soaking for 24 hours, the first 4-6 hour soak it with 10% bleach water. your pc tek is long enough..i go 90min@15lbs have no problem since. but i do have this feeling the problem is from the lc...does the contam follow the same path as the injected lc or is it all over?
peace
siam


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Offlineke1n
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Registered: 11/15/05
Posts: 359
Last seen: 14 years, 6 months
Re: 3rd Attempt @ WBS jars - CONTAMED?? [pic] [Re: Thai_connection]
    #5314634 - 02/18/06 09:07 PM (17 years, 11 months ago)

thanks Tai Connection

It looks like it does in many of the jars. I remember this happening to spore-injected jars as well though..


anyway would it be possible to pour water/h202 into the jar and shakei t around and let it sit for a few hours then drain it out? i know that would alter the water content but would there be a chance that the contam would be killed as well?


--------------------



Everything that is posted, including pictures and text, are a result of fictional storytelling using images found online and/or created using the latest graphics software. I am a fictional writer who likes to explore the internet world.
------------------------------------

http://www.adobe.com/


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OfflineSinthetic
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Registered: 12/05/05
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Last seen: 15 years, 6 months
Re: 3rd Attempt @ WBS jars - CONTAMED?? [pic] [Re: ke1n]
    #5318895 - 02/20/06 08:23 AM (17 years, 11 months ago)

I had to exact same stuff in my first WBS jars. At first I thought it was mycelium but after a few days they started to stink so I chucked them.


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Offlinelardnar
Pu Pu Platter
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Registered: 06/15/05
Posts: 703
Loc: Behind what appears
Last seen: 16 years, 11 months
Re: 3rd Attempt @ WBS jars - CONTAMED?? [pic] [Re: Sinthetic]
    #5321495 - 02/20/06 08:05 PM (17 years, 11 months ago)

I am real big on playing around trying to save jars (never works) but I would toss that one


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