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OfflineDelinquentes
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LACK of cleanliness in our hobby.....
    #5314108 - 02/18/06 05:56 PM (17 years, 11 months ago)

I'd like to hear from a few people here about your LACK of cleanliness in preparing jars and whatnot.

Tell me your worst case scenerio including the results.

The reason I ask is I just laughed at myself.....I walked in to my clean room and it smellls EXACTLY like a hospital. Hell, I even wiped my PC with 71% isopopyl before taking it through the door. I also sprayed the outside of the spore syringe bag with lysol before taking it in to the room.

Am I just a freak, or not?


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



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Offlinerevbear
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Re: LACK of cleanliness in our hobby..... [Re: Delinquentes] * 1
    #5314185 - 02/18/06 06:19 PM (17 years, 11 months ago)

Wow, I wish I could afford a clean room. I must settle for a clean kitchen and a homemade glove box, and I have housemates so basically keep the nature of my shroomery to myself, in the closet.

love, revbear


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OfflineDarkMark
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Re: LACK of cleanliness in our hobby..... [Re: revbear]
    #5314203 - 02/18/06 06:25 PM (17 years, 11 months ago)

Lol Ive made jars that didnt contam right next to a glass of moldy liquid (didnt realize this till i was done) with a dirty counter top I did spray some oust in the air though.


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Offlinejamman
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Re: LACK of cleanliness in our hobby..... [Re: revbear]
    #5314210 - 02/18/06 06:26 PM (17 years, 11 months ago)

I dont clean my room at all before I knock up jars. I simply wipe down my glovebox with bleach and alcohol, then spray my pc'd jars with the 10% bleach once there in the box. Works fine for me.

There is a big difference between being clean and being sterile tho. There is no need to have an entire room rubbed down with alcohol if your not going to be doing open air transfers or injections.


--------------------
Common Misspelled Words


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Offlinehyphae
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Re: LACK of cleanliness in our hobby..... [Re: jamman]
    #5314220 - 02/18/06 06:29 PM (17 years, 11 months ago)

I wipe down everything that enters my cleanroom. I have about a .2% contam rate over the last 20yrs.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineRogerRabbitM
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Re: LACK of cleanliness in our hobby..... [Re: hyphae]
    #5314403 - 02/18/06 07:34 PM (17 years, 11 months ago)

When dealing with spawn and cultures, one can never be too sterile. Nobody posting above is going beyond the call of duty. It must be recognized however, before we burn ourselves out trying to keep EVERYTHING sterile, there is no need to be sterile in the actual growing environment. Once your substrate is fully colonized, ordinary room air won't hurt a thing. I chuckle when I see polyfil on the vents in a terrarium for example.
RR


--------------------
Download Let's Grow Mushrooms



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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinedjsage420
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Re: LACK of cleanliness in our hobby..... [Re: RogerRabbit]
    #5314407 - 02/18/06 07:36 PM (17 years, 11 months ago)

I put ployfil over my vents so to slow air exchange or else my RH would drop.


--------------------
If you put your head to the grass, you can hear it growing


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Offlinehyphae
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Re: LACK of cleanliness in our hobby..... [Re: RogerRabbit]
    #5314512 - 02/18/06 08:23 PM (17 years, 11 months ago)

Quote:

RogerRabbit said:
When dealing with spawn and cultures, one can never be too sterile. Nobody posting above is going beyond the call of duty. It must be recognized however, before we burn ourselves out trying to keep EVERYTHING sterile, there is no need to be sterile in the actual growing environment. Once your substrate is fully colonized, ordinary room air won't hurt a thing. I chuckle when I see polyfil on the vents in a terrarium for example.
RR



I chuckle when I hear peoples casings are contaminating with trich. I always try to do my best keeping everything as clean as possible but I don't go overboard I know what it takes. Learning sterile culture technique also means practicing cleanliness even in your everyday chores in otherwords making it not only a habit but second nature. Of course I'm only referring to serious growers. Much respects RR


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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InvisibleRahz
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Re: LACK of cleanliness in our hobby..... [Re: Delinquentes]
    #5314657 - 02/18/06 09:14 PM (17 years, 11 months ago)

Don't take my procedures as recomendations, I'm posting simply because I'm probably one of the more DIRTY growers.

There's stuff I do that is essential, and a lot of stuff I don't do. When innoculating, I flame my needle, and keep it wrapped in an alcohol soaked paper towel. Once removed from the towel, it spends about 1 second in the open air before being plunged into the jar. I use injection ports made from RTV sealant. I wipe my injection point down with alcohol before inserting the needle. I wash my hands.

That's all I do. My jars cool down in the kitchen with dirty plates. I don't wipe anything down, spray the air, or wear a mask. I inoculate them on the counter with the AC on, and sometimes with food cooking on the stove. Since I started using the injection ports, my contam rate has been 2% (1 jar) and that was due to wet tyvek during incubation.

I get away with this because the sterile environment in the jars and syringe never comes into contact with the outside air. For me it's worth it to spend 5 minutes making 12 inoculation ports, so that I can avoid the work in creating a sterile environment. I use ports on my LCs as well.

If I ever get into agar, I'll have to change my ways, but that's how I do jar inoculation. Again, I'm sloppy and take my chances, but there is something to be said for pushing the limits. Getting contams can be viewed as an excellent learning experience, just be safe about handling them.

Rahz


--------------------
rahz

comfort pleasure power love truth awareness peace


"You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi


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OfflineSolidcell
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Re: LACK of cleanliness in our hobby..... [Re: Rahz]
    #5314930 - 02/18/06 10:55 PM (17 years, 11 months ago)

I fill my jars on the counter in the hallway, and I inject over my desk in a carpeted room after wipping the counter with bleach solution and spreaying that solution above the desk. I have had no contams other than all the EQs that came contam in the needle, I got a prompt replacement tho. However, I've only done 24 qt jars so far.


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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InvisibleliloldmeFacebook
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Re: LACK of cleanliness in our hobby..... [Re: Rahz]
    #5314944 - 02/18/06 10:59 PM (17 years, 11 months ago)

I wash my hands, take a shower, runa hepa in the grow room, thats it, I'll get a contaminated casing after like 4 flushes, but by them i dont care, I really feel like I should work harder on being clean, but I really dont have a problem. for example, When casing, I wash my hands every 5 casings, no problems, cuts down on the work...


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OfflineDinahTheCat
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Re: LACK of cleanliness in our hobby..... [Re: Solidcell]
    #5314946 - 02/18/06 11:00 PM (17 years, 11 months ago)

Everyone growing should use the universal precaution procedures, forgot what they are, but its pretty much the cleanier the better, less risker or worrysome with precautions.... i mean hell one time i without watching my hands picked out a mycelium cake from one jar and planted it into a jar that was not cleaned out and still smelled of tabasco pepper jelly(mmmmmmm :smile: ) This was a long time ago, and was very irresponsible, i mean the odds were against me. Im just saying yeah, its possible you could do it without bringing everything you come in contact with in quaritine, but the safer the better. peace and good luck/.


--------------------
"Sanity is not statistical."



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OfflineDelinquentes
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Re: LACK of cleanliness in our hobby..... [Re: DinahTheCat]
    #5314983 - 02/18/06 11:11 PM (17 years, 11 months ago)

Keep them coming folks! I am enjoying hearing all of the different situations. Someday, somebody is gonna find this thread as interesting as I do now.


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


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OfflineDinahTheCat
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Re: LACK of cleanliness in our hobby..... [Re: Delinquentes]
    #5315002 - 02/18/06 11:16 PM (17 years, 11 months ago)

hahha now i have a "decontamination" center where i enter before working in the labratory, then i put on my sterile vest, visor, gas mask with 02, gloves, pants, and thong. I then enter my lab, and forget what the hell i was gonna do.


--------------------
"Sanity is not statistical."



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Invisibletiny_rabid_birds
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Re: LACK of cleanliness in our hobby..... [Re: DinahTheCat]
    #5315080 - 02/18/06 11:32 PM (17 years, 11 months ago)

Quote:

hahha now i have a "decontamination" center where i enter before working in the labratory, then i put on my sterile vest, visor, gas mask with 02, gloves, pants, and thong. I then enter my lab, and forget what the hell i was gonna do.




You're kidding right? That's a bit over the top if you ask me, but hey, if you've got the means to do it, more power to you.


--------------------


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InvisibleliloldmeFacebook
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Re: LACK of cleanliness in our hobby..... [Re: tiny_rabid_birds]
    #5315207 - 02/19/06 12:03 AM (17 years, 11 months ago)

sounds like so crap I would do DinahTheCat, do you put on a condom as well?


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OfflineDinahTheCat
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Re: LACK of cleanliness in our hobby..... [Re: liloldme]
    #5315425 - 02/19/06 01:18 AM (17 years, 11 months ago)

Soooo crap hhahahha did you see i put in thong? nah i pull out man.


--------------------
"Sanity is not statistical."



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OfflineHoss
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Re: LACK of cleanliness in our hobby..... [Re: DinahTheCat]
    #5315456 - 02/19/06 01:31 AM (17 years, 11 months ago)

I don't do anything.

I work in a dusty, nasty, stuffy, moldy basement.

I dont' take a shower before I work, I don't wash down any surfaces...

The only sterility I have is using a gauze pad soaked in isopropyl and wrapping that around the syringe needle when I inoculate.

Actually, I also spray some lysol or something when I am making a casing :smile:.


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OfflineDelinquentes
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Re: LACK of cleanliness in our hobby..... [Re: Hoss]
    #5315547 - 02/19/06 02:52 AM (17 years, 11 months ago)

Are you serious Hoss?


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


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InvisibleliloldmeFacebook
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Re: LACK of cleanliness in our hobby..... [Re: Delinquentes]
    #5315616 - 02/19/06 03:32 AM (17 years, 11 months ago)

im sure he is serious, that the main time to be anal about this hobby, other that using a sterile medium...


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OfflineHoss
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Re: LACK of cleanliness in our hobby..... [Re: liloldme]
    #5315624 - 02/19/06 03:36 AM (17 years, 11 months ago)

Yeah I'm serious.


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Invisibleshroomparty
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Re: LACK of cleanliness in our hobby..... [Re: Delinquentes]
    #5315721 - 02/19/06 06:47 AM (17 years, 11 months ago)

I work out of my dirty, smelly, moldy (mostly the good mold) basement.

I PC brf jars and needles, I don't PC anything else. I case cakes to 100% verm straight out of the bag. I use lysol and a mask when taking prints or making innoculant, never otherwise - same can has lasted 6 months+

I put a little h202 in all my water - evaporative humidifier, ultrasonic humidifier (have both), heating tub(s). I wash my hands before most procedures, when I remember. My contam rate was around 5% until the last batch I did all got contams, bad inoculation syrum.

I don't own a glove box.

I also re-use my dunking water, but throw it out after 3 days or so.

Jars that get contams get soaked in scalding water + bleach before being filled up with BRF mix again.


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OfflineCubenisseur
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Re: LACK of cleanliness in our hobby..... [Re: shroomparty]
    #5316001 - 02/19/06 10:19 AM (17 years, 11 months ago)

Quote:

I have about a .2% contam rate over the last 20yrs.



Wow, thats dating yourself!! I didn't realize that they even HAD mushrooms back then!  :shocked: :tongue2:  :crazy2: :wink:


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OfflineHeffy
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Re: LACK of cleanliness in our hobby..... [Re: Cubenisseur]
    #5316021 - 02/19/06 10:28 AM (17 years, 11 months ago)

My grow room right now is in a friends place. The apartment is squalid and doing any kind of sterile lab technique is just about impossible. I will let you know if I have any success. If I do it'll be quite something, the place is disgusting.


--------------------
I am the king of Rome, and above grammar! - Emperor Sigismund


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Offlinehyphae
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Re: LACK of cleanliness in our hobby..... [Re: Heffy]
    #5316125 - 02/19/06 11:09 AM (17 years, 11 months ago)

I would like to say I take my hobbies very seriously to the point of being anal it has served me well. I got into the hobby more for learning sterile culture technique than anything else, it was fun I got off each and every time I entered my cleanroom all gloss white everything setup perfectly and in it's place just rushed me out. Doing transfers whether agar, G2G or making up 100's of spawn bags I was in heaven knowing I have my bases covered. I should have been a lab worker! I know this thread is about LACK of cleanliness but I just have to say getting too sloppy will bite you guys in the ass when you least expect it or want it too and consistency well that is out the door whether you are sincere about it or not. I have learned from the best and take pride in everything I do whether it's creating or taking a shit! Remember I'm anal :wink: LOL

A mentor and colleague who takes his shit seriously also:




:wink:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinedoc34
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Re: LACK of cleanliness in our hobby..... [Re: hyphae]
    #5316145 - 02/19/06 11:17 AM (17 years, 11 months ago)

Holy Fucking Shrooms!!!!!


Awesome Pics hyphae !!!


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InvisibleRoadkillM
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Re: LACK of cleanliness in our hobby..... [Re: Delinquentes]
    #5316210 - 02/19/06 11:40 AM (17 years, 11 months ago)

I'm almost Anal about keeping things clean and sterile.

but...

I have a good friend that used to work in the nastiest conditions and hardly ever got any contams.
I'd watch him do syringe making and culture work in open air with his cat walking over his shit.
I watch him work in his nasty ass kitchen with dirty dishes stacked up in the sink and nasty floors.
And he never wiped the counters that he was working on.
:crazy:

his results were...
he was one of the best cultivators I have ever seen.

go figgure!~


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineDinahTheCat
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Re: LACK of cleanliness in our hobby..... [Re: Roadkill]
    #5316606 - 02/19/06 02:18 PM (17 years, 11 months ago)

Dude hypae you are amazing! I wish i could grow like that, haha you must get shit loads of goodies!


--------------------
"Sanity is not statistical."



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OfflineOatman2000
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Re: LACK of cleanliness in our hobby..... [Re: DinahTheCat]
    #5319583 - 02/20/06 11:23 AM (17 years, 11 months ago)

i have a sort-of Clean room....

Actually i have a Grow-Room. I have a roommate who is dirty, and does not take the time to consider the hobby period.

He just wants it now, and wants it yesterday... his life is too busy to even make Jars, he just waits for me to do it.

He never reads whole articles through even when i PM them right to his mailbox here.

Everything i have tried to keep clean becomes contaminated, when he walks into the room while smoking a cigarette, carying his tray from upstais to downstairs.

Asks me why his stuff isn't colonizing.... well i am just done. Never should have went through the process of showing.

Other than that i have sucess even considering the facts... But lately i am having a bacterial infection plaging all my jars.

It's hard to keep things clean, when you don't have someone's full attention... and constently hearing the words "I'm sorry" come out of their mouth. Makes me wanna Bitch Slap him every time.

This could happen to you!


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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OfflineAnarchysAvatar
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Re: LACK of cleanliness in our hobby..... [Re: Oatman2000]
    #5319629 - 02/20/06 11:37 AM (17 years, 11 months ago)

Holy contams Batman!


--------------------
"Every Revolutionary Was Once Considered An Enemy"




Anarchy's Avatar - Out


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Re: LACK of cleanliness in our hobby..... [Re: Oatman2000]
    #5319690 - 02/20/06 12:03 PM (17 years, 11 months ago)

Quote:

oatman2000 said:





MMMMMMMMMMM! What's for dinner! :smile:


--------------------

Quick WBS Prep


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OfflineDelinquentes
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Re: LACK of cleanliness in our hobby..... [Re: FooMan]
    #5319728 - 02/20/06 12:12 PM (17 years, 11 months ago)

There seems to be no rhyme or reason to all this...some are pigs and have terrific luck, some are uber-clean and still have trouble...interesting.

One other thing I forgot to mention about my setup was the fact that I ran a home-made ozone generator in the room for an hour to really clean the room out(while the HEPA was going too). Ozone is one of the most aggressive contaminant killers known to man.

I'm not the cleanest person in the world, but I am constantly running the HEPA and the ozone in the house because I smoke. I hate the smell of my own habit and the HEPA and Ozone kills it completely.

I even ran the Ozone in my car for 24 hours...it smells like a new car now and I don't sneeze when I turn on the A/C anymore.

It's a ghetto ozone maker...maybe I will post a picture...I'll tell you how to make it if you want.



Edited by Delinquentes (02/20/06 12:41 PM)


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InvisibleRahz
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Re: LACK of cleanliness in our hobby..... [Re: Delinquentes]
    #5319795 - 02/20/06 12:31 PM (17 years, 11 months ago)

>>>>It's a ghetto ozone maker...maybe I will post a picture...I'll tell you how to make it if you want.

I'm curious.

Rahz


--------------------
rahz

comfort pleasure power love truth awareness peace


"You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi


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Offlinehyphae
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Re: LACK of cleanliness in our hobby..... [Re: Rahz]
    #5319904 - 02/20/06 01:11 PM (17 years, 11 months ago)

Nice generator! But be careful guys high levels of ozone are extremely hazardous to your health and can fry your lungs and any other living thing around ie; plants, shrooms, wife, kids, etc; :wink:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineDelinquentes
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Re: LACK of cleanliness in our hobby..... [Re: hyphae]
    #5319920 - 02/20/06 01:17 PM (17 years, 11 months ago)

Yes...I am aware.. I am posting a tek about it and have added that warning to it...

Thanks!


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



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