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Offlineymhrswrider
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Question for those who have grown a few times concerning contams.
    #5310425 - 02/17/06 01:09 PM (17 years, 11 months ago)

First off this is a question for those of you out there who have grown multiple times, not the kids who did PF Tek twice and are now an expert.
Both myself and a friend have grown enough times to be fairly good at it. We both use a modified bulk neglect method that uses rubbermaid containers as the case and TiT for heat. We both use popcorn and we use Tenn Stud for our poo. We are both very clean throughout the process. The only contact we have concerning the grow is that we share each others pressure cooker, which is cleaned with hot soapy water each use. We don't really ever hang out at each others house and we've only seen each others grow once.
-Now here is my question. A few grows back, say 4 months or so, I started getting invaded with mold(mainly green) in my jars. I started loosing between 80-100% of my grows before I ever got them spawned to the poo. Then I started loosing casings after 1 or 2 flushes. Then before I even got to 1 flush. This progressivly got worse over those months. Nothing had changed from what I was doing when things were going good with no contams. I started trying new things to combat the contams like scrubbing EVERYTHING that would be even close to my grow with bleach, using different prints, using longer pressure cooker times, ect... Nothing worked. Then my buddy tells me he is getting the same problem. We are two intelligent guys. We have sat down and tried to figure this out with no luck. There should have been no way that we could have cross contaminated our grows. He is getting the same green mold is his jars. Now my last grow (so far at least) is doing fine. Only one jars had any contam. So, has wnyone else run into sort of the same thing? Have you got a rash of contams that just cleared up? What have you done to change your method to fix this?
We have run down every possibility and can't find anything that stands out as a problem. I did find out that I was running my incubator a bit hot at 92 degrees. Would this be a possible cause? maybe putting too much stress out the myc while being a perfect environment for mold? Let me know your thoughts. Thanks guys.


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Flashbacks are God's way of saying, "This one's on me".


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InvisibleFungusMan
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Re: Question for those who have grown a few times concerning contams. [Re: ymhrswrider]
    #5310444 - 02/17/06 01:16 PM (17 years, 11 months ago)

Its called "paragraphs". You should try it! :smile:


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Offlineymhrswrider
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Re: Question for those who have grown a few times concerning contams. [Re: FungusMan]
    #5310466 - 02/17/06 01:24 PM (17 years, 11 months ago)

Yep. I'll jump right on that.


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Flashbacks are God's way of saying, "This one's on me".


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Re: Question for those who have grown a few times concerning contams. [Re: ymhrswrider]
    #5310568 - 02/17/06 01:59 PM (17 years, 11 months ago)

Often, when one begins gets great results from his first couple grows, he often begins taking short cuts, cutting corners & such.

MOLD IN SPAWN JARS.

Could be failure to properly sterilize grains (to short PC times).

Could be used syringes not properly sterilized.

Could be contaminated inoculates.

Could be poor inoculation procedure.

Could be ineffective gas exchange filtration.

Could be contaminates wicking in through G/E filter.

Go over those points.
You have to get clean spawn, before anything else.


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InvisibleliloldmeFacebook
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Re: Question for those who have grown a few times concerning contams. [Re: agar]
    #5310596 - 02/17/06 02:07 PM (17 years, 11 months ago)

I would like to bet on the syringes not being clean.


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Offlineymhrswrider
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Re: Question for those who have grown a few times concerning contams. [Re: liloldme]
    #5310724 - 02/17/06 02:44 PM (17 years, 11 months ago)

Syringes(plus water inside) were PCed at 15lbs for 1 hr once the weight started wobbling. Syringes were always left to cool off in the pc overnight. Used gas stove to heat needle before mixing water with spores(sealed inside an air tight food saver bag that was wiped down with rubbing alcohol). Needle was heated to red hot everytime before it went into something new(jars, spore baggy, ect).


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Invisibleagar
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Re: Question for those who have grown a few times concerning contams. [Re: ymhrswrider]
    #5310900 - 02/17/06 03:58 PM (17 years, 11 months ago)

Quote:

ymhrswrider said:
Syringes(plus water inside) were PCed at 15lbs for 1 hr once the weight started wobbling. Syringes were always left to cool off in the pc overnight. Used gas stove to heat needle before mixing water with spores(sealed inside an air tight food saver bag that was wiped down with rubbing alcohol). Needle was heated to red hot everytime before it went into something new(jars, spore baggy, ect).




SPORES FROM DIRTY PRINTS.......MAYBE  :confused: :rolleyes:


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OfflineOatman2000
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Re: Question for those who have grown a few times concerning contams. [Re: agar]
    #5310923 - 02/17/06 04:06 PM (17 years, 11 months ago)

you say you had in incubatior (tit style) going at 90 degreeze?

that's high... close enough to kill myc, and bring in unwanted bacteria.

you also say that your using your tit as a FC? your incubating your casings? spanned to poo?

Poo generates it's own heat, and should not be incubated. Trich likes warmer climates.

IMO - i think you should knock a few jars, and place them in different locations around your house... ie) incubator, cabinet above fridge, bedroom, bathroom, kitchen.

see if these grow. i have done this, and i found out that i had a temp problem. but i didn't get trich, i got lipstick.


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WBS QUART SPAWN JAR PREPERATION
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4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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Offlinehyphae
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Re: Question for those who have grown a few times concerning contams. [Re: ymhrswrider]
    #5310934 - 02/17/06 04:13 PM (17 years, 11 months ago)

Sounds like you may have a pretty good sterile culture technique. Are the PC's the only thing you share? Think about it, as agar suggested contaminated spores? Are you guys buying from the same supplier? Also during certain times of the year and with certain environmental conditions (lots of rain) spore loads rise significantly, was this outbreak during a period of high moisture? Also often times when contams break out indoors it gets much worse before it gets better.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlineymhrswrider
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Re: Question for those who have grown a few times concerning contams. [Re: hyphae]
    #5311022 - 02/17/06 04:39 PM (17 years, 11 months ago)

I'm thinking it's from the high incubator temps. Unfortunately it's very cold where I live and the house stays at about 66deg usually so I have to use a TiT. As far as spores and supplys, I used multiple prints from multiple grows. I turned down the temp quite a bit and so far no contams. I was mainly looking for if anyone else had a long rash of similar stuff happen like oatman2000.


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Flashbacks are God's way of saying, "This one's on me".


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Invisibleagar
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Re: Question for those who have grown a few times concerning contams. [Re: ymhrswrider]
    #5311210 - 02/17/06 05:15 PM (17 years, 11 months ago)

Spring is the generally the worst time for contam's. Reason why, is that grass, plants, shrubs & tree's produce pollen & release it into the air.

Other spores of all types (including mold) attach themselves to pollen, one way or another, then blow around everywhere.

So, the spore load in everyones surroundings, usually increases dramaticaly.

One other thing to watch out for is where/how you handle & dispose of contaminated jars, spawn & substrates.

If you carry contaminated things out to the garbage can, dumpster, compost pile, or whatever & empty them out. You can be spreading a heavy contaminate spore load all along that path, as well as all over your clothing & yourself.

WHICH CAN/WILL come back to haunt you, for months & months.

If you insist on salvaging the lids & jars that have contaminated?

Best to fill a tote about 5/8ths full of sudsy / bleachy / pinesol / lysol water (or, any such combo). Then, submerse the jars, twist open the lids - enough that the jars fill. Then, let then soak overnight in that sudsy / bleachy / pinesol / lysol water.

The following day, clean/scrub out the jars & lids, pour the tote full of gunk in the toilet & flush it. Then, run the jars & lids through the dish-washer.

If you insist on savaging funky trays?

Best to spray the contaminated substrate heavily with a sanitizing agent, slide the tray into a garage bag, hold the neck of the bag closed, dump tray out. Remove tray, close bag, seal bag with twist tie & discard in trash can.

Then, soak trays in a tote filled with sudsy / bleachy / pinesol / lysol water - overnight. Then, scrub out & dry.

In either case, always try to contain & minimize the possible spore load - you may be spreading all over the place. Always change into cleanly laundered clothes, after dumping out funky jar/trays. Then, launder those clothes & take a hot soapy shower - yourself.

Also, change your furnace filters - as often as once a month. You might even think about having your duct - work - cleaned out.


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Offlineboosthappyvw
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Re: Question for those who have grown a few times concerning contams. [Re: agar]
    #5311932 - 02/17/06 08:33 PM (17 years, 11 months ago)

i would switch from popcorn to something else atleast for some jars and see if that helps i always had poor sucess using popcorn and even certain brands of wbs. i also heard it is good for the shrooms to have a change of nutes


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Invisibleagar
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Re: Question for those who have grown a few times concerning contams. [Re: boosthappyvw]
    #5311990 - 02/17/06 08:52 PM (17 years, 11 months ago)


BIRD BASICS BRAND @ PetsMart, 40 lb bags = $8.


Soak 24 / 30 hours


Rinse, drain & jar up.


PC min 60 minutes, preferably 90 @ 15/18 psi


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Offlineymhrswrider
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Re: Question for those who have grown a few times concerning contams. [Re: agar]
    #5313095 - 02/18/06 11:22 AM (17 years, 11 months ago)

Thanks for the info AGAR. I usaually reuse my jars and empty them out in the back yard. Didn't even think about that being a source of problems. I'm hoping this grow is the end of my worries.


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Offlinehyphae
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Re: Question for those who have grown a few times concerning contams. [Re: ymhrswrider]
    #5313453 - 02/18/06 01:52 PM (17 years, 11 months ago)

It's amazing how nature takes care of contams outdoors it most always gives the myc the upper hand. Your friend also dumps his OD too? Spores are everywhere alright your covered with them right now! :wink:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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