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Offlinerygo796
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Registered: 04/17/05
Posts: 221
Last seen: 15 years, 6 months
This sound like myc in my LC
    #5310066 - 02/17/06 11:02 AM (17 years, 11 months ago)

I got a few white puffs inside my LC...looks kinda like white puffs of germinated spores when i do WBS...is this what I'm looking for in my LC and if it is how long should I wait to use it?


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OfflineWronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
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Re: This sound like myc in my LC [Re: rygo796]
    #5310090 - 02/17/06 11:10 AM (17 years, 11 months ago)

That sounds correct. The amount of time you need to wait really depends on your method of agitation. I use a stir plate for my liquid cultures so they can usually be ready for use in 5-7 days. If you are shaking or simply swirling them around your time may be around 7-10 days.

When you have a nice cloud covering at least 40% of the liquid, you'll be ready for use following a good shake or swirl to break them up.


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Invisibletiny_rabid_birds
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Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
Re: This sound like myc in my LC [Re: Wronguy]
    #5310152 - 02/17/06 11:36 AM (17 years, 11 months ago)

Wronguy, when using a stir plate for LCs, how often and for how long do use use it?


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OfflineWronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
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Re: This sound like myc in my LC [Re: tiny_rabid_birds]
    #5310203 - 02/17/06 11:49 AM (17 years, 11 months ago)

I stir my liquid cultures every evening for 1-2 minutes. I just make sure the vortex is going all the way down to the bottom of the jar and wait the allotted time. Works great.


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