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Amazon Shop: ½ Pint Jars

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Offlinesillvyr
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Registered: 01/12/02
Posts: 125
Loc: under a tree
Last seen: 9 years, 5 months
Nothing Has Happened!
    #530962 - 01/25/02 01:36 PM (15 years, 10 months ago)

Hey Y'all,
This is so weird. CaptainMaxMushroom said I might've contaminated my jars, but if I did, there is no sign. There is also no sign of an mycelium, either. Five days and absolutely nothing at all. Been incubating at 82-85 degrees, pretty steady, too. I'm getting a little worried that my babies are not going to grow this time. I contacted the vendor about this (Sporeworks), and my contact there has been awesome. He's been so nice and very helpful. I highly recommend them to anyone. He's so nice that he will replace my spores if I don't see growth by this weekend. I only hope this is not my fault, but it probably is, I'm so damned inexperienced I botched it without even trying to. It's hard to mess up the first time because I've lost some faith in myself.


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The "Little Mushrooms" are but emisaries to the Overmind.....



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Offlineduss69
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Registered: 11/27/01
Posts: 71
Last seen: 11 years, 8 months
Re: Nothing Has Happened! [Re: sillvyr]
    #530969 - 01/25/02 01:45 PM (15 years, 10 months ago)

Lo
Wait a bit more before worrying....
Spores can take up to 10-15 days to show signs of growth.
Depends on age of spores and conditions of incubations.
My First ever jat took about 10 days before showing mycelium.
After that time begin to worry seriously.
Patience is the key with our little friends.

++
Duss69


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Offlinesillvyr
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Registered: 01/12/02
Posts: 125
Loc: under a tree
Last seen: 9 years, 5 months
Re: Nothing Has Happened! [Re: duss69]
    #531039 - 01/25/02 03:16 PM (15 years, 10 months ago)

Hey,
Duss69, you just breathed some hope into the project! It's comforting to know that all is not lost yet, and that maybe my baby mushies are just slow to wake up. I guess I'm too impatient. By the way, that first time, did you use a syringe or did you make one from a spore print? I made my own from a print, and maybe that's the hold-up, that the spores were rather dry to begin with. Thanks for sharing your experience.
Sending happy vibes for healthy mushies!


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The "Little Mushrooms" are but emisaries to the Overmind.....



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OfflineShroomZilla007
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Registered: 01/25/02
Posts: 13
Last seen: 15 years, 8 months
Re: Nothing Has Happened! [Re: sillvyr]
    #531076 - 01/25/02 03:58 PM (15 years, 10 months ago)

Hey there dude,

First: "Spores can take up to 10-15 days to show signs of growth."

You prolly read that somewhere when you first started this hobby...
Think about it.  How long have you waited?  How long can spores take?

Poor Captain Max......

And, if your jars are showing NO GROWTH, it is NOT contam.....
Think about that to....

I would bet it's your fault one way or another,  and that you should
give Max some leeway and actually give your you spores a chance to germinate
before you go talking to him about it...
If you are growing pf style, RE-READ the manual...

* sheesh! *

Oh, and GOOD LUCK!!!

:smile:

 


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OfflineHammerloaf
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Registered: 11/26/01
Posts: 439
Loc: Canada
Last seen: 14 years, 4 months
Re: Nothing Has Happened! [Re: ShroomZilla007]
    #531194 - 01/25/02 06:21 PM (15 years, 10 months ago)

One thing you said caught my attention... you used a print?
how did you innoculate the jars, via syringe?


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Offlinesillvyr
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Registered: 01/12/02
Posts: 125
Loc: under a tree
Last seen: 9 years, 5 months
Re: Nothing Has Happened! [Re: Hammerloaf]
    #531341 - 01/25/02 09:13 PM (15 years, 10 months ago)

Some clarifications in response to the last 2 posts that
addressed mine:
First: I have NEVER read that germination would take up to 10-15 days. Every guide has indicated some observed growth in 5 days, those were ones that I have read on this site.
Second, CaptainMaxMushroom did NOT supply me with spores, I simply posted and he responded.
Last, about the print thing, I made my own syringes from the print.
If I have contaminated my jars, as you insist I have, I thought that at times contamination might be detectable by odor only, and visible contamination is not the sole means by which to diagnose it.


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OfflineHammerloaf
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Registered: 11/26/01
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Re: Nothing Has Happened! [Re: sillvyr]
    #531794 - 01/26/02 10:48 AM (15 years, 10 months ago)

well.. could be that you didn't wait 12 hours for the spores to rehydrate after making the syringe.. umm.. it maybe that the spores were crap :smile:

hmm.. possibly it's contaminated.. but unless there is SOMETHING visible I highly doubt it... unless of course it smells really strange.. like fresh baked blueberry pie.. mmmm....


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Anonymous

Re: Nothing Has Happened! [Re: sillvyr]
    #531803 - 01/26/02 11:00 AM (15 years, 10 months ago)

With Psilocybe Cubensis spores, I have found if you don't see some spots of mycelium growth by the 5th or 6th day you probably never will. Sorry, just my 2 cents.


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Offline_JJ_
PsychedelicRevolutionary
Registered: 06/12/01
Posts: 369
Loc: NSW, Australia
Last seen: 2 years, 5 months
Re: Nothing Has Happened! [Re: Hammerloaf]
    #531806 - 01/26/02 11:01 AM (15 years, 10 months ago)

Maybe you didn't prepare the syringe properly? Did you boil the water you used? Did you wait long enough for it to cool down before mixing in the spores? Did you use alcohol to clean the shot glass and not wait for it to dry? Did you get the water content right in the substrate? Are the jars incubating in a warm chamber? Also if you didn't wait for the spores to reyhdrate you can lengthen germination time as Hammerloaf said. Give it a couple more days I'd say :laugh:


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OfflineCabee
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Registered: 09/24/01
Posts: 49
Last seen: 15 years, 10 months
Re: Nothing Has Happened! [Re: Hammerloaf]
    #531808 - 01/26/02 11:03 AM (15 years, 10 months ago)

If someone was able to(in sterile conditions) have the spores fall directly in about 50cc of water rather than make a print on paper or in a jar and have to fill the jar with water later.Wouldn't you be able to use the spore filled water right away?Would you still have to wait 12 hours?


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OfflineMattycelium
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Registered: 11/11/01
Posts: 126
Last seen: 10 years, 5 days
Re: Nothing Has Happened! [Re: Cabee]
    #531972 - 01/26/02 02:03 PM (15 years, 10 months ago)

When you buy a print , the spores are not fresh, like dried, so then need to be hydrated again, just like any seed for any plant...need H2O. To your question-yes wait 12hrs least...and i would recommend putting them in a syringe


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Offlinesillvyr
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Registered: 01/12/02
Posts: 125
Loc: under a tree
Last seen: 9 years, 5 months
Re: Nothing Has Happened! [Re: Cabee]
    #532137 - 01/26/02 04:44 PM (15 years, 10 months ago)

Hey guys,

well, it looks as though something is starting to happen, although I'm not exactly sure what. I see some spots growing, they look white, except for one spot that looks different--either it is new and a thin layer, or it is some yucky contaminant. It looks less than white, almost silvery; which is probably bad but I want to let it go longer and see what happens. Most of the rest of the jars show absolutely nothing at all still, though. Thanks for answering my questions.


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Offlineduss69
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Registered: 11/27/01
Posts: 71
Last seen: 11 years, 8 months
Re: Nothing Has Happened! [Re: ShroomZilla007]
    #532486 - 01/27/02 12:04 AM (15 years, 10 months ago)

>>Hey there dude,
>>First: "Spores can take up to 10-15 days to show signs of >>growth."
Lol, you're right i was a bit enthusiastic here....
What i wanted to say is that it can sometimes take more than 5 days ...
My first jar took about 10 days before showing positive signs of mycelial growth.
And 100% agree with you to say that when nothing happens , dont blame the vendor first!!!!
It is more likeky that you have messed up...

++
Duss69


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Offlinesillvyr
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Registered: 01/12/02
Posts: 125
Loc: under a tree
Last seen: 9 years, 5 months
Re: Nothing Has Happened! [Re: sillvyr]
    #532937 - 01/27/02 02:11 PM (15 years, 10 months ago)





Hey Guys,
well, sad news. that spot turned ugly overnight, as you can see. should I try to save the mycelia that is good:

[image]http://shroomery.org/wwwthreads/files/12861-alsomycelia.jpg[/image
, you know, say in sterile water and use to inoculate other jars later? Or is this just a bad iea? The spots are on opposte sides of the jar, and do not touch each other yet. Any suggestions?


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Anonymous

Re: Nothing Has Happened! [Re: sillvyr]
    #532979 - 01/27/02 02:51 PM (15 years, 10 months ago)

You can try to salvage what you can by taking a sterile spoon and digging the area out that looks contaminated. It's not 100% but worth a try.

A note on rehydrating spores: Whether they were rehydrated or not makes no difference. Once they are injected into your jar, theyare rehydrating. Your substrate holds water, the spores were shot in with water, they will rehydrate in the jar.


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Amazon Shop: ½ Pint Jars

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