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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlinerygo796
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Registered: 04/17/05
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Agar to WBS, no glove box?
    #5308098 - 02/16/06 06:16 PM (17 years, 11 months ago)

I have some peroxide agar with some good growth on it and was wandering if at all there is a good way to do wedge to grain without a glovebox. I was considering using my kitchen and some lysol with everything sterilized with alcohol but I am not sure if this will be sterile enough.


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Offlinedjsage420
aka DJQBNSIS
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Registered: 02/15/05
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Re: Agar to WBS, no glove box? [Re: rygo796]
    #5308102 - 02/16/06 06:18 PM (17 years, 11 months ago)

Turn off all fans in your house, even your fridge, and spray lysol or oust, wear a dust mask on your face, and do it as fast as you can the less time it takes the less likley a contam will float in.


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OfflineSnaggletooth
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Re: Agar to WBS, no glove box? [Re: rygo796]
    #5308116 - 02/16/06 06:24 PM (17 years, 11 months ago)

Yeah I would spray the air with a 10% bleach solution, wipe everything down, I would try not to move the air with fans.

Gloves and alcohol, Flame the loop or whatever your using.

And make it quick with less moves necessary, first plain every move that you need to do beforehand.

Keep the lids on the jar and dish open only as your transferring, then close.

Then make the transfer quickly.

Good Luck :thumbup:


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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