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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Gas generation during incubation.
    #5306575 - 02/16/06 11:58 AM (17 years, 11 months ago)

I have seen that some of you make special 'gas exchange' ports on your incubation jars. I'm wondering if that is neccessary at this point. Are harmful gasses like CO2 forming during the incubation stage? Has anyone used completely sealed incubation jars and had success or failure?

~Cappa.


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Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Offlinethenewguy05
The Mushroom Man
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Re: Gas generation during incubation. [Re: cappa]
    #5306584 - 02/16/06 12:02 PM (17 years, 11 months ago)

i have attempted sealed jars but without oxygen the mushrooms don't grow. died within a week; at least I think it did.

gas exchange is one of THE MOST IMPORTANT things in this hobbie next to patience.


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Gas generation during incubation. [Re: thenewguy05]
    #5306606 - 02/16/06 12:08 PM (17 years, 11 months ago)

Thank you for your fast response.
Im talking about the incubation stage, colinization-myc growth. I wouldn't expect mushrooms to grow in a sealed chamber. Are you saying that CO2 is generated BOTH during incubation and fruiting?

~Cappa.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Offlinethenewguy05
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Re: Gas generation during incubation. [Re: cappa]
    #5306669 - 02/16/06 12:20 PM (17 years, 11 months ago)

co2 is generated in every aspect of the growth for mushrooms.
incubation, spawning, and fruiting


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Gas generation during incubation. [Re: thenewguy05]
    #5306677 - 02/16/06 12:22 PM (17 years, 11 months ago)

That is what I needed to know my friends, thank you.

~Cappa.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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InvisibleOmnicracker
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Registered: 09/18/05
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Re: Gas generation during incubation. [Re: cappa]
    #5306701 - 02/16/06 12:26 PM (17 years, 11 months ago)

your jars will stall (the myc. will stop gowing) if there is no exchange.


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xxx..Learn Something..xxx


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Invisiblecappa
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Registered: 02/12/06
Posts: 854
Re: Gas generation during incubation. [Re: Omnicracker]
    #5306711 - 02/16/06 12:27 PM (17 years, 11 months ago)

Again, thank you for your quick reply.

~Cappa.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Invisibletiny_rabid_birds
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Re: Gas generation during incubation. [Re: cappa]
    #5306789 - 02/16/06 12:42 PM (17 years, 11 months ago)

If you're just doing PF jars though, you don't have to worry about filter lids. Just make sure you have the inch layer of pure vermiculite to act as a filter and leave the 3-4 inocc points uncovered, and that will be all the gas exchange you need for incubating BRF jars.


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Offlinehyphae
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Re: Gas generation during incubation. [Re: cappa]
    #5306807 - 02/16/06 12:44 PM (17 years, 11 months ago)

The only thing thats really necessary is that you crack the lids so that they are not air tight all that filter shit is really totally unnecessary during the incubation stage. People just like to make things more complicated than need be, me included!


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Invisibletiny_rabid_birds
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Re: Gas generation during incubation. [Re: hyphae]
    #5306841 - 02/16/06 12:49 PM (17 years, 11 months ago)

The filters do serve dual purposes though, since they act as inocc points, so it's not that much of a hassle to have them.


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Invisiblecappa
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Registered: 02/12/06
Posts: 854
Re: Gas generation during incubation. [Re: tiny_rabid_birds]
    #5306914 - 02/16/06 01:06 PM (17 years, 11 months ago)

Thank you all for replying.

Now let me explain why I asked the question.
Im planning on using WBS for incubation. I wanted to make my jars using 4 small holes filled with silicone. That will enable me to inoc and they will basically reseal themselves. This seemed to me first of all, a far simpler method of inoc and second of all, a more sure-fired way of maintaining sterility since the seal is never broken except by a sterilized needle.

A problem arises if gas exchange needs to take place though.

~Cappa.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Invisibletiny_rabid_birds
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Re: Gas generation during incubation. [Re: cappa]
    #5306930 - 02/16/06 01:10 PM (17 years, 11 months ago)

Well in that case, you can just take hyphae's advice and loosen the lids a bit during incubation.  Although you really only need one inocc point on grain jars, since you shake the shit out of them anyways to distribute the spores.  Really no reason to put four.

But yeah, that seems fine to me.  Resealable silicone nocc point with loosened lids for incubation :thumbup:


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Offlinehyphae
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Re: Gas generation during incubation. [Re: tiny_rabid_birds]
    #5306977 - 02/16/06 01:20 PM (17 years, 11 months ago)

They may be convenient to some (me) they are not really necessary a hole with tape over it also works well for inoculation points. I've done it many ways and I have to say IMHO it just makes this hobby a little more interesting but not necessary although some teks do benefit more than others, I myself like unimpeded gas exchange so I use them often. And like you said they can be used to inoculate thru.
I also agree one inoculation point is all you want bro!


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineJaicen
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Re: Gas generation during incubation. [Re: hyphae]
    #5307368 - 02/16/06 02:56 PM (17 years, 11 months ago)

I've always thought this is a strange idea. Since CO2 is denser than air, it's gonna want to stay inside the jars. There's not way it's just gonna exchange through a filter without assistance.


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Gas generation during incubation. [Re: Jaicen]
    #5307409 - 02/16/06 03:07 PM (17 years, 11 months ago)

Good call on the point about shaking the jars and such. Thank you for your comments.
Good point Jaicen! You would have to invert the jars to actually get the CO2 out through your inoc points.

Well that settles it, unless Agar or Hippiechick says I have to have the gas exchanges, they're gone.
Actually, let me settle this once and for all, I will install gas exchanges on half of my jars, and half without. That way I'll be giving something back to you guys. I don't like to just leech knowledge without giving something back.

~Cappa.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Offlinehyphae
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Re: Gas generation during incubation. [Re: cappa]
    #5307894 - 02/16/06 05:23 PM (17 years, 11 months ago)

FYI vegetating myc loves CO2 guys. What you need to understand is in a sealed environment the gases generated by the myc during the digestion of substrate buildup pressure as well as metobolic waste (myc piss) both of which are detrimental to healthy cultures in excess and can promote anaerobic contamination. Flipping the jars isn't done to release CO2 it only helps with gas exchange a bit. I hope you guys are understanding what is actually happening here? And Jaicen it will exchange bro through means of pressure that is produced by the myc. I really hope this gives you guys a better understanding. GL and stay safe


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


Extras: Filter Print Post Top
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