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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
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Vacutainer Question
#5297321 - 02/13/06 10:17 PM (17 years, 11 months ago) |
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i don't wanna transfer any LC to vacutainers prematurely. so, two questions...
-how thick, as in how much myc, should the LC be that goes into the vacutainer?
-obviously, the purpose of a vacutube is to preserve LC or spore solution for an extended or indefinite period of time. but, if you make them sparse, if they're left at room temp (or even incubated) can the myc continue to grow in the tube?
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
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Re: Vacutainer Question [Re: krill]
#5297340 - 02/13/06 10:23 PM (17 years, 11 months ago) |
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It dose not matter how much LC but more the better in the Vac.
As far as spores goes you should use distilled water, and the spores still could germinate, but not as likely and another way to prevent that is keeping them cool/dark.
Have you looked into Slants?
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Atheist Chat
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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
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what's a slant?
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: Vacutainer Question [Re: krill]
#5297354 - 02/13/06 10:29 PM (17 years, 11 months ago) |
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Resurrecting a Better Method for Long-Term Storage of Mushroom Cultures
Quote:
Under aseptic conditions, simply dip a sterile loop into the vial, *** and streak the mycellia-rich water onto an agar plate. It will start to reanimate on being in a nutrient source and oxygen.
The first four methods keep cultures alive with three items: food, water, and oxygen. If they lack any of these, It's goodbye. Instead of trying to keep them alive,there is a better way: In sterile distilled water, with no food, oxygen, or minerals.
And check out Storing Strains Long Term
And Sterile Culture Technique
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Atheist Chat
Edited by Snaggletooth (02/13/06 10:38 PM)
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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
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thanks Snaggle
i duno if i'm ready to take on all of that yet...
more than anything i planned on using my vacu's to store LC and spore solution. i just wasn't sure how much myc is NECESSARY...
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: Vacutainer Question [Re: krill]
#5297388 - 02/13/06 10:42 PM (17 years, 11 months ago) |
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I agree I have not done it yet, but just letting you aware of it.
Yeah there is no set amount, but more the better.
I did a transfer from a LC to a Vac, now there was very little LC, and I tried but there was none, but there was so little LC to start with, I thought I would try it.
As long as you can see a bunch it's good, I on the other hand tried to get just a few 'strings' and failed.
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Atheist Chat
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Vacutainer Question [Re: krill]
#5297414 - 02/13/06 10:49 PM (17 years, 11 months ago) |
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For long term LC culture storage in a vacutianer.
I suggest you inject a heavy myc laden LC solution.
Stand tube on end for 24 hours.
Which allows myc to settle into the bottom of the tube.

Then, syringe aspirate out as much liquid as possible. Leaving as much myc as possible.
Then, inject sterile distilled water.

Which removes much of the available nutrient source.
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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
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Re: Vacutainer Question [Re: agar]
#5298948 - 02/14/06 11:53 AM (17 years, 11 months ago) |
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let me re-ask the question. say i have a few WEAK LC's in bottles of Karo solution. what if i aspirated a mild nute (karo and/or honey) solution into the vacutainers (say, half full) then PC them for how ever long, then nocc them w/ a small amount of myc??? in theory, would this allow me to make a number of vacutainer sized LC's from one weak Baby bottle LC???
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
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Wronguy
Registered: 03/05/05
Posts: 4,450
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Re: Vacutainer Question [Re: krill]
#5298961 - 02/14/06 11:56 AM (17 years, 11 months ago) |
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Yes and no. Yes because your introducing live culture to a sterile nutrient source. No because growth will be limited inside of a vacutainer. There is no avenue for CO2 expulsion so your growth will stall, if it grows at all.
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: Vacutainer Question [Re: krill]
#5298963 - 02/14/06 11:57 AM (17 years, 11 months ago) |
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Yes and no.
If you are worried that you have to little Mycelium then you might want to wait.
*edited* did not see that it was answered...slow typing....lol
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Atheist Chat
Edited by Snaggletooth (02/14/06 11:58 AM)
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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
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yeah, i figured the idea itself would work in the right environment... and obviously if there's no air exchange AT ALL then it's not the right enviromnment...
is there a possibility that a few of my bottles aren't getting AE for some reason?
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
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Wronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
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Re: Vacutainer Question [Re: krill]
#5299051 - 02/14/06 12:21 PM (17 years, 11 months ago) |
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It's a very strong possibility if you're using baby bottles. I tried baby bottles back in the day and they just didn't work well. AE was the likely culprit here. Shoot Agar a PM, I'll bet he has some ideas for you.
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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
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Re: Vacutainer Question [Re: Wronguy]
#5299092 - 02/14/06 12:34 PM (17 years, 11 months ago) |
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thanks Wronguy. like i say, some of the BB's are whooping ass, but some just can't get going - even w/ big chunks of fresh spores at the bottom.
i will shoot Agar a PM here in a while...
e/z
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
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