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tokey666
Let's Build It Instead


Registered: 08/05/05
Posts: 666
Last seen: 2 years, 8 months
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sooo when is my liquid culture completely colonized?
#5297180 - 02/13/06 09:36 PM (17 years, 11 months ago) |
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Thanks to agar and his simple tek, I now have a few baby bottles colonizing....and there are little floaties in there.....but...how do I know when it's done? Does it become a solid mass of....floaties?
Thanks!
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Wronguy
Registered: 03/05/05
Posts: 4,450
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Re: sooo when is my liquid culture completely colonized? [Re: tokey666]
#5297187 - 02/13/06 09:38 PM (17 years, 11 months ago) |
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You should have a nice formation of clouds inside your baby bottles. Here is a pic of a colonized LC, except it's malt/dex:

Gives you an idea of what to look for.
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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
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Re: sooo when is my liquid culture completely colonized? [Re: Wronguy]
#5297266 - 02/13/06 09:59 PM (17 years, 11 months ago) |
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so, in your experience, Wronguy... will a Karo LC get this thick?
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
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shroomsynergy
Fungi Freak


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Re: sooo when is my liquid culture completely colonized? [Re: krill] 1
#5297295 - 02/13/06 10:09 PM (17 years, 11 months ago) |
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it should get pretty close. i've taken culture out for use even when it's only half that thick, and just leave the rest alone for later. when your culture is almost done growing you will usually notice somewhat of a clearing of the liquid. this kinda signals that your culture has used most of the nutrients available. then its time to store in the fridge, or use it up.
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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
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Re: sooo when is my liquid culture completely colonized? [Re: shroomsynergy]
#5297298 - 02/13/06 10:11 PM (17 years, 11 months ago) |
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"you will usually notice somewhat of a clearing of the liquid. this kinda signals that your culture has used most of the nutrients available."
that's another catch-22 with the Karo method. it's clear from start to finish...
will growth just stop?
what's the LONGEST it's taken anyone to get a fully colonized baby bottle LC using Karo?
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
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shroomsynergy
Fungi Freak


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Re: sooo when is my liquid culture completely colonized? [Re: krill]
#5297377 - 02/13/06 10:38 PM (17 years, 11 months ago) |
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sorry, i forgot, i usually use plain honey. not karo. soory bout that. dough!!! 
i think my first one took the longest cause i eyeballed the mix, and made it too strong. abot 3 1/2 weeks. usually in premo conditions i get like two weeks till i start using it. (it's just my results though, i dont know what the norm is.)
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Edited by ShrOOmSynEgry (02/13/06 10:41 PM)
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: sooo when is my liquid culture completely colonized? [Re: tokey666]
#5297390 - 02/13/06 10:43 PM (17 years, 11 months ago) |
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These have gas exchange filters & were stirred with a stir plate. Yours may not get that thick. But, once you have a golf ball sized (=/-) cloud in it, It's good to go.
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Wronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
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Re: sooo when is my liquid culture completely colonized? [Re: krill]
#5298445 - 02/14/06 08:56 AM (17 years, 11 months ago) |
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Quote:
krill said: so, in your experience, Wronguy... will a Karo LC get this thick?
In my experience, a Karo liquid culture does not perform as well as light malt extract and dextrose. I've used both and pretty much abandoned Karo after I tried the latter. Now this is just my experience. Yours may vary. Agar may have some good performing Karo LC photos to show.
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pipeDream
Moooo

Registered: 02/08/06
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Re: sooo when is my liquid culture completely colonized? [Re: Wronguy]
#5298541 - 02/14/06 09:53 AM (17 years, 11 months ago) |
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Im new to these liquid cultures. When it is completely done colonizing you can just pull the contents up into a syringe and inoculate the jars this way? Really what I am asking is how do you use a completely colonized liquid culture. I assume you cant use the brown rice flour and verm for something like this.
-------------------- What is wrong with our culture is that it offers us an inaccurate conception of the self. It depicts the personal self as existing in competition with and in opposition to nature. We fail to realise that if we destroy our environment, we are destroying what is in fact our larger self. (Freya Matthew)
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Wronguy
Registered: 03/05/05
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Re: sooo when is my liquid culture completely colonized? [Re: pipeDream]
#5298561 - 02/14/06 09:58 AM (17 years, 11 months ago) |
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The colonized liquid culture can be used to inoculate any substrate appropriate for cultivating. The biggest advantage to using a liquid culture spawned to a substrate is you are skipping the germination phase of the spawned medium. Spore germination will occur in the LC only.
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pipeDream
Moooo

Registered: 02/08/06
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Re: sooo when is my liquid culture completely colonized? [Re: Wronguy]
#5299176 - 02/14/06 01:13 PM (17 years, 11 months ago) |
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Im a little confused here, it seems like if you inoculate brown rice flour and verm with this instead of just the spores that you still need to incubate it and let the jar completely colonize with the mycelium. What do you mean you skip the germination phase?
-------------------- What is wrong with our culture is that it offers us an inaccurate conception of the self. It depicts the personal self as existing in competition with and in opposition to nature. We fail to realise that if we destroy our environment, we are destroying what is in fact our larger self. (Freya Matthew)
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Wronguy
Registered: 03/05/05
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Re: sooo when is my liquid culture completely colonized? [Re: pipeDream]
#5299208 - 02/14/06 01:20 PM (17 years, 11 months ago) |
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The spores have already germinated inside the liquid culture. You skip the germination process when you spawn to, say your BRF, by adding a living culture. Incubation is required for the mycelium to continue colonizing the substrate you just introduced it to.
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tokey666
Let's Build It Instead


Registered: 08/05/05
Posts: 666
Last seen: 2 years, 8 months
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Re: sooo when is my liquid culture completely colonized? [Re: Wronguy]
#5299224 - 02/14/06 01:26 PM (17 years, 11 months ago) |
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Basically pipeDream, it's faster. It shaves 2-5 days off of germination times, and also will make one spore print make 20 needles, instead of just 1.
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pipeDream
Moooo

Registered: 02/08/06
Posts: 67
Last seen: 17 years, 10 months
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Re: sooo when is my liquid culture completely colonized? [Re: tokey666]
#5299249 - 02/14/06 01:36 PM (17 years, 11 months ago) |
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Ahh i understand... i was just forgetting about the time it takes between inoculation till the first sign of mycelium growth. Sounds very useful. Thanks everyone, will have to look more into this topic.
-------------------- What is wrong with our culture is that it offers us an inaccurate conception of the self. It depicts the personal self as existing in competition with and in opposition to nature. We fail to realise that if we destroy our environment, we are destroying what is in fact our larger self. (Freya Matthew)
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tokey666
Let's Build It Instead


Registered: 08/05/05
Posts: 666
Last seen: 2 years, 8 months
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Re: sooo when is my liquid culture completely colonized? [Re: pipeDream]
#5300160 - 02/14/06 05:42 PM (17 years, 11 months ago) |
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Cool, let me ask another question if anyone is still watching this thread.....
How often do I shake UNTIL colonized?
Furthermore, when I make syringes, do I just suck of the culture? Or are there viable spores in the uncultured water too?
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
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Re: sooo when is my liquid culture completely colonized? [Re: tokey666]
#5300311 - 02/14/06 06:41 PM (17 years, 11 months ago) |
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I agitate the container twice a day.
But if you want to go nuts check this out Constant-stir LC showing expotential growth in first 3.5 days. (pics)
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Atheist Chat
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magic8ball
Stranger
Registered: 02/06/06
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Re: sooo when is my liquid culture completely colonized? [Re: Snaggletooth]
#5344700 - 02/27/06 09:59 AM (17 years, 10 months ago) |
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1) what is a stir plate and where can you buy one?
2) do you have to sterilize un-used empty syringes or can you just suck the LC right up?
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thenewguy05
The Mushroom Man


Registered: 02/11/05
Posts: 2,123
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Re: sooo when is my liquid culture completely colonized? [Re: magic8ball]
#5344717 - 02/27/06 10:03 AM (17 years, 10 months ago) |
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a stir plate simply stirs the liquid culture for the predetermined amount of time so you don't have to shake it like usuall. Ebay usually has one for cheap.
if the syringe is brand new out of the package then you can just use it.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: sooo when is my liquid culture completely colonized? [Re: magic8ball]
#5344914 - 02/27/06 11:14 AM (17 years, 10 months ago) |
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Quote:
magic8ball said: 1) what is a stir plate and where can you buy one?
2) do you have to sterilize un-used empty syringes or can you just suck the LC right up?
 Stir plate, spinning a stir bar in a quart jar of water.
 LC's that were stirred by that method
If syringes are in a sterile wrapper, you are good to go.
  
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