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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Bday Suprise
#5294136 - 02/13/06 09:30 AM (17 years, 11 months ago) |
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just got off vacation, and came home to my jars...
i made them a different way this time... using polyfill instead of tyvek. I got the same thing! nasty baccilus looking contam. or lipstick mold, either way this time it wasn't the tyvek.
i am about sick of grains. i think i am gonna go back to doin cakes.
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Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Flippetdlongtime
So Solid Crew

Registered: 12/06/05
Posts: 110
Last seen: 17 years, 10 months
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I feel your pain bro. I had real good results using Equador syringe from The Hawk's Eye when I inoculated 2 quart jars of WBS with it. They were fully colonized in 12 days. I used a Treasure Coast syringe from The Hawk's Eye on 4 quart jars of WBS and the growth is, sad to say, but very poor. I had inoculated 2 other quart jars of WBS using Golden Teacher syringe from The Hawk's Eye and they never even had a chance to grow. I got the foot smell coming from both jars within a week. When I examined the jars closer, it looked as if all the millet had exploded. But the other 6 jars were fine. Perhaps they fermented because of too much water and popped? I have no clue, this hobby is still a guessing game to me, so only 25% success rate out of my first WBS try. A little disappointed but there is always tomorrow.
Oh, forgot to say that I used a tyvek filter AND a polyfil stuffed hole on all jars.
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Edited by Flippetdlongtime (02/13/06 09:54 AM)
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Cubenisseur
Mad Props


Registered: 12/04/05
Posts: 1,392
Loc: Indian Land
Last seen: 13 years, 10 months
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Happy birthday!
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mediman0078
Stilllooking.....

Registered: 11/14/05
Posts: 1,379
Loc: Here, there, EVERYWHERE
Last seen: 17 years, 10 months
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WBS is filled with endospores.
Soak 24 hrs Rinse Simmer for 40 min. Rinse Jar up and let sit for another 24... if there's a load of water at the bottom of the jars, dump it out now. PC for 1.5 hours Let cool in PC Ready everything for innoc, then crack the PC and do the innocs quick and clean. Place in a clean box and leave them alone for another 10 days. Don't go checking on them every couple hours... you're just inviting contams doing that. After 10 days you should be able to tell if anything went bad... if you were careful, nothing should've.
-------------------- ........someday I'll find it.
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coda
Banjo Goiter


Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
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need to correct this : "Jar up and let sit for another 24... if there's a load of water at the bottom of the jars, dump it out now."
Dont let grains sit if they havent been pc'ed\
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
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Rahz
Alive Again


Registered: 11/10/05
Posts: 9,230
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Are you innoculating through the polyfil? PCing for at least 1.5 hours? I know it's probably dumb questions, just trying to help.
You should try my tyvek injection ports (in my journal). After assembling the jars, there is no point at which the grains are exposed to open air until birthing. No materials other than the jar lid and sealant are directly exposed to the grains. The tyvek stays dry, they're cheap, easy to make, give me peace of mind, and when I inoculate, it's nice to have the injection port firmly holding the syringe upright.
/end salesman pitch. Seriously, give them a try before you give up on grains.
Rahz
-------------------- rahz comfort pleasure power love truth awareness peace "You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: Bday Suprise [Re: Rahz]
#5294714 - 02/13/06 01:05 PM (17 years, 11 months ago) |
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yes... i have used tyvek, and hate it. i am thinking that is where i am getting the most of my contams. So i went back to polyfill... then went on vacation, to come home to the same thing!
only step i can say would be bad, is that i am doing innoculations in front of the oven.
i set at 150 degreez, and then inoculate. I hate to fiddle with my glovebox... but it looks like i'm gonna have to go back.
i am spending my jet lagg, b-day remaking my jars. This is not what i wanted to do. this is a love-hate relationship with this hobby.
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Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Rahz
Alive Again


Registered: 11/10/05
Posts: 9,230
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Well, I attribute my success to the fact that my grains are _never exposed to a peep_ of outside air.
I laugh at glove boxes (for this specific purpose). I don't shower or change clothes. I don't clean anything down, including jars, surfaces. I don't attempt to sanitize the air. I have a bale of straw sitting in the next room. I use clean hands and sterile syringes, and I swab the tyvek with alcohol before penetration, that's it. If you use tyvek with inoculation ports, you SHOULD have the same success that I've had which would have been 100 percent. I had a single jar (out of 48 so far) contam because I kept the foil on tight, and the tyvek got saturated. Now I just take the foil off after PCing.
Others may prefer other methods, but this has worked well for me. I'd hate to see someone give up on grains. Tyvek isn't the problem, it's application and procedure.
Rahz
-------------------- rahz comfort pleasure power love truth awareness peace "You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi
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coda
Banjo Goiter


Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
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Re: Bday Suprise [Re: Rahz]
#5295401 - 02/13/06 03:32 PM (17 years, 11 months ago) |
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Thats some good advice rahz, dont give up on something thats not working for you. Never blame the arrow, always look at the indian so to speak. Check your procedure out, are you making jars from the syringe and they contam? Have you ever done a control jar? Try switching brands of WBS? Do you keep the needle in an alc. soaked cloth between injections?
BTW what Tek are you following anyways?
Happy bday as well!
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
Edited by coda (02/13/06 03:33 PM)
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finding_self
Stranger
Registered: 05/15/05
Posts: 489
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yu
Edited by finding_self (04/23/06 07:03 AM)
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campinman
Fun-Guy


Registered: 09/01/05
Posts: 398
Loc: SoOn
Last seen: 7 years, 3 months
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happy birthday!!!
wish you got a sweet flush instead of lipstick mold.
all the best man, if at first you don't succeed, do a search and read read read!
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction. "I wish my lawn was emo, so it would cut itself."
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Fick_Duck
Truffle Shuffle


Registered: 10/18/02
Posts: 247
Loc: The Hamburger Train
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Re: Bday Suprise [Re: campinman]
#5296048 - 02/13/06 05:50 PM (17 years, 11 months ago) |
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Quote:
campinman said: all the best man, if at first you don't succeed, do a search and read read read!
I like that motto!
-------------------- "To know life you must fuck it in the liver." -Dr. Frankenstein, Andy Warhols Frankenstein
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finding_self
Stranger
Registered: 05/15/05
Posts: 489
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sorry
Edited by finding_self (04/22/06 09:25 PM)
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Quote:
oatman2000 said: only step i can say would be bad, is that i am doing innoculations in front of the oven.
Oven tek is BS. FACT...Heat CONVECTION draws in cool outside air.
First off, sounds like your grain jars are TO WET.
Get some MICROPORE TAPE. Use it as a lid filter.
Open air innoc.
Get some STERILE gauze pads (small box-full is cheap). (if need be use a folded paper towel)
Open a couple pads, pour on some 70% alc.
Pour some alc in a small dish, near you. (for use in the process - later)
When you unsheathe a spore or LC syringe needle.
IMMEDIATELY wrap needle with alc soaked pad.
KEEP NEEDLE WRAPPED IN ALC PAD AT ALL TIMES.
Use other alc pad to swab lid.
Holding the needle in the pad. (takes a little practice)
Place needle (enclosed in pad)over lid hole.
Press pad against lid.
Push needle into hole (with pad around it on outside of lid).
Push needle INTO lid hole. (needle hub still surrounded bu pad)
Squirt in inoculate.
Withdraw needle, with pad surrounding needle.
Dip a pre-cut piece of micropore tape in alc dish.
Tape over injection hole with alc soaked micropore tape. (it will stick)
Proceed to next jar & repeat same process.
This innoc method minimizes needle exposure to OPEN AIR.
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musher_420
Stranger

Registered: 08/01/05
Posts: 2,691
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Hey, Sounds like me man.
I got the GREEN. Then I had lip stick. Then Bacterial contams TWICE in a row. I was doing SOMETHING wrong.
So I made some changes. I let my grains drain REALLY good. I usually PC'ed for an hour. I switched that up to hour and a half. And I made sure to cover my needle with an alcohol soaked paper towel in between inoculations.
Guess what... PROBLEM SOLVED!
Do you have a Rocker weight PC or a Gage type? If you just have a rocker weight. You may not be getting the full 15 P.S.I that you need.
Happy Birthday either way!
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campinman
Fun-Guy


Registered: 09/01/05
Posts: 398
Loc: SoOn
Last seen: 7 years, 3 months
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its copyrighted. find yer own. lol
contams can only come from so many angles though. maybe the original syringe where the spores are comin from is contammd. then yer fucked no matter how many layers of tyvek and coffeefilters you strap on there
could be the oven tek, i thought that sounded good, then i realize i havent cleaned my oven in a while, and even if i did use that oven cleaner vile smelly stuff, i wouldnt wanna 'noc up jars with those fumes blowing into my face
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction. "I wish my lawn was emo, so it would cut itself."
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: Bday Suprise [Re: campinman]
#5296772 - 02/13/06 08:20 PM (17 years, 11 months ago) |
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yea my oven isn't the cleanest of things.... but it's not that dirty either. the only thing i cook in it is casing mix. (i am a microwave man)
i do take an alcahol soaked papertowel and wipe each needle before inoculation, but i do not "sheeth" it in the towel. (will do this for this batches innoculations.
i am using/have tried a variety of teks..... i have read soo much i would have thought my eye's have bleed. (what my g/f thinks... "you on that damn computer again")
But here it is in a nutt shell....
24hr soak rye grain drained water...rinsed thoroughly under showerhead. refilled pot with water and placed on stovetop. Waited till i saw a slow boil, (simmer) then removed from heat. Let the grains sit in hot water for 2 hrs. to absorb moisture dumped out in strainer and let dry till no drips are comming out.
i then dumped it into a sterilite dishpan tub, and added chunky vermiculite, because i didn't want hardly any water (everything has always been too wet IME) the grains look good and dry, but plump from the steep.
i then load the quart jars 2/3 full, and this time i used a polyfill filter due to the fact that my experience with tyvek has been 3 months of trouble.
Loaded jars... polyfill filter, and toped with alum foil. Stuck them in my "rocker type" PC (with a guage) btw... it pc's at 17 psi.
in my pc i fill a Bunn-O-Matic pour thinggie full of water, this is what i use to steralize my jars... anything less would evaporate too quickly. and PC 7 jars at a time.
PC for 1 hr +, and take off heat and let PC's cool to room temp (20 min)
i open the pc, and shake the jars to distribute the grains. (there is really no difference but just give em a quick shake)
i then procede to let them cool on kitchen table with the box lid over top of them... still has alum foil over tops.
Next day.... wake up and do innoculations in front of oven, Preheat oven to 150 degreeze, and pull up a chair.
I do 6 jars at a time, with 2cc's of LC in each Qt. jar.
i place the jars on the lid of the oven, and then remove the foil. take my syringes, and remove the cap and wipe the needle down with alc soaked paper towel. i then plunge the needle into the polyfill and squirt down the side of the jar.
immediatelly coffee filters are placed overtop of the jars, and securred with a rubberband. the jars are then moved to the island countertop to await labeling this is all done very quickly, due to the fact that the oven coil will "kick" back on to heat back up. time frame = 2 min.
i have experimented with incubating the jars... not incubating the jars. and splitting up the bunch in different locationg around my house to see the differences in colinization.
every single jar looks like this...
  
don't get me wrong... it looks and starts as rhizzo helthy myc, then a week into it... Whammo! smell, and contams. What's the deal
Please critique my procedure..
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Rahz
Alive Again


Registered: 11/10/05
Posts: 9,230
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>>>>it looks and starts as rhizzo helthy myc, then a week into it... Whammo! smell, and contams.
This really sounds like what would happen if endospores survived the PC. I noticed in the pics that your contams are down in the jar, this could also indicate pre-existing contams. You might try PCing for 2 hours or even 2.5 hours.
If you're innoculating through the polyfil, I think you're fine there. I also think that if contams were introduced during or after inoculation, you would have some kind of success rate. With a good method like you're doing, if all your jars are going bad, this too would point to seed that didn't get sterilized. Longer PC times, that's where I'd put my money.
Rahz
-------------------- rahz comfort pleasure power love truth awareness peace "You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: Bday Suprise [Re: Rahz]
#5298316 - 02/14/06 07:35 AM (17 years, 11 months ago) |
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i agree.... but i am afraid that i will boil all the water out of my pc.
i will just keep doing tests.... i just called and had another bag of rye ordered... so 56 lbs later, if still no success. I might give up for a while.
*** here's another thng too. i have found that my rye seeds germinate very quickly. a 24hr. soak tends to start the germination process....
so i also did what Stamets said, and took dry grain in the jar... added water, and pc'd that. On this run i made about 5 different variations. We'll see what happens between them.
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Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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You may want to try a steaming soak to start off with. The heat will stop the rye from germinating and it will absorb water MUCH faster this way. Get a pot of water boiling, turn off the heat and add the rye to it. Put a lid on the pot and let it soak like that.
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Quick WBS Prep
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