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InvisibleAnand
AutisticShroomer
I'm a teapot User Gallery

Registered: 11/05/02
Posts: 366
Loc: The netherlands
Trusted Cultivator
Cloning Black truffle on agar
    #5293788 - 02/13/06 05:02 AM (17 years, 11 months ago)



A good friend of mine got that one from work, its too old to sell and the good taste is gone but can I clone it on agar as with mex a or tampa?

or does it needs plant roots to grow as with Amanita's


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OfflineJeremy_Davis
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Registered: 04/22/05
Posts: 652
Loc: Florida
Last seen: 11 years, 9 months
Re: Cloning Black truffle on agar [Re: Anand]
    #5293799 - 02/13/06 05:30 AM (17 years, 11 months ago)

I will say yes, but I'm not truthfully sure. In all likelihood, it will work for you. Maybe even try 2 or three formulations of agar to vary the pH and nutrients available. Sclerotia are a powerful genetic resivoir, so you should have all the necessary DNA for successful cloning. Do it quickly, as the fruitbody degrades, the window for successful tissue culture closes. Let us know how it goes! I'd be interested to hear what your experiments with this species are, and how they go. I'd ask for a culture, but I'm not sure I'm on the right lat lines to match the needs. Cool to start your own orchard though!
Light and Love,
Jeremy Davis
Educationall Concerns for Hunger Organization, Inc.
www.echotech.org/greta


--------------------
Jeremy Davis
Educational Concerns for Hunger Organization, Inc.
Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta


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