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InvisibleDT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
Pinning problem
    #5293595 - 02/13/06 01:14 AM (17 years, 11 months ago)

I made a couple of spawn bags and had them fully colonize. I took the huge cakes out of the bags and proceeded to slice some of them into sizable slabs (2-3cm in thickness) and the rest, broke into marble sized clumps. I then placed the slabs & clumps into tinfoil trays which were subsequently placed inside a terrainum each. On the bottom of the terraniums is damp perlite. In the lid I have cut a hole 10cm in diameter tightly covered it with a microfilter. The insides of the tupperware container is very much covered with condensation. The temperature inside the terraniums is 24c (76F).

I left them in such a state in total darkness for 2 days in which time some fluffy mycelium began forming ontop and throughout the other mycelium.

At this point, I placed 100 watt incandescent light bulbs about 5 feet away and cycling in bursts of 2 hours for a total of 8 hours a day.

It has been a week in such a condition and now there are some white thin mycelia darting out of the surface of the mycelium. From what I understand those are pins.

My problem is that there does not seem to be nearly enough of them as there should be for such a large surface area. None are browning. From what I have read, a week into the light cycles, the pins should be browning. To add to my sad state, it seems that the condensation from some of the terraniums has dripped onto the surface of the cakes causing a small buildup of water there. I dabbed it with some paper towel to remove the standing water.

And here are the questions I'd really like answered.

1) Could my problems be because I allowed only 2 days for the fluffy mycelium to form? Will the fluffy mycelium form if I turn off the lights?

2) What do I do about the cake whose surface was wet from condensation falling on it, and now hardened?

3) Is there something just plain wrong about my process that causes these problems?


I'm sorry for making this post so long, but I really need some help here. Thank in advance for any suggestions.


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Offlinedjsage420
aka DJQBNSIS
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Registered: 02/15/05
Posts: 869
Loc: Panhandle
Last seen: 17 years, 10 months
Re: Pinning problem [Re: DT2K]
    #5293749 - 02/13/06 04:16 AM (17 years, 11 months ago)

Ok first off, you can fruit at 76f, but it isnt advisable thats almost inccubation temp, lower the temp to 71f,
Give the lid to your fruit chamber a very light coat of vasolene (petrolium jelly) this will keep water from sticking to it, building up and then falling on your cakes.
Also you dont mention anything about a window or any way for the light to get in. Go buy a cheap picture frame at the dollar store, cut a whole a little smaller than the glass on picture frame, connect glass over the whole with silicone... (you can also lightly vasolene the window also)
Also I have waited 2 weeks for pins before, when you get them there wont be a doubt in your mind what they are.


--------------------
If you put your head to the grass, you can hear it growing


Edited by djsage420 (02/13/06 04:17 AM)


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Pinning problem [Re: djsage420]
    #5293809 - 02/13/06 05:49 AM (17 years, 11 months ago)

Quote:

djsage420 said:
Ok first off, you can fruit at 76f, but it isnt advisable thats almost inccubation temp, lower the temp to 71f,
Give the lid to your fruit chamber a very light coat of vasolene (petrolium jelly) this will keep water from sticking to it, building up and then falling on your cakes.
Also you dont mention anything about a window or any way for the light to get in. Go buy a cheap picture frame at the dollar store, cut a whole a little smaller than the glass on picture frame, connect glass over the whole with silicone... (you can also lightly vasolene the window also)
Also I have waited 2 weeks for pins before, when you get them there wont be a doubt in your mind what they are.




Just need to correct some misinformation here:
Incubation temps: 84-86F.
Fruiting temps: 74-77F.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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InvisibleDT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
Re: Pinning problem [Re: hyphae]
    #5294222 - 02/13/06 10:10 AM (17 years, 11 months ago)

The tupperware that I'm using is Rubbermaid, and transparent and at some parts translucent, light can get in just fine. I use 100 Watt bulbs as a light source as well.

Tell me what pins exactly look like if you'd be so kind. Are they the white & thin mycelia poking out from the surface? Mind you, they do look like a pinhead I'm just not sure if it's them.


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Offlinehyphae
born to grow
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Posts: 6,228
Loc: the rain forests
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Re: Pinning problem [Re: DT2K]
    #5294394 - 02/13/06 11:21 AM (17 years, 11 months ago)

No that is most likely just rhizo myc growth, pins look like tiny white dots.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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InvisibleDT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
Re: Pinning problem [Re: hyphae]
    #5295615 - 02/13/06 04:09 PM (17 years, 11 months ago)

Ok, thanks.

One last very important question though, and this is an important one. Keeping in mind that I have no pinning a week into the light cycles, can I just break up all of the cakes and use them for casings? Will that work at all?

I assume it should since none of the nutrients have really been used up. Moreover, I'd prefer the casing method because I no longer have confidence in the cakes that were soaked from condensation.


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Offlineonetime
onetime
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Registered: 11/13/03
Posts: 3,609
Last seen: 13 years, 1 month
Re: Pinning problem [Re: DT2K]
    #5295630 - 02/13/06 04:12 PM (17 years, 11 months ago)

Quote:

DT2K said:


At this point, I placed 100 watt incandescent light bulbs






Most people dissagree with me But I would never ever ever ever use incandescent light bulbs. This things are evil. They are bad enough on human eyes. I think lights have little efect on mushrooms. Florecents are what I use.


--------------------

See?
Yes, with my own three eyes.
Depression, Misspells , wanting everying thing i cant have haveing nothing i want


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
Trusted Cultivator
Re: Pinning problem [Re: DT2K]
    #5298175 - 02/14/06 06:02 AM (17 years, 11 months ago)

I'd leave your cakes a few more days pins will come soon. I also like fluoros.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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