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Offlinekike
Spirit chaser
Registered: 02/08/06
Posts: 4
Loc: europe
Last seen: 17 years, 9 months
LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!!
    #5290718 - 02/12/06 03:02 AM (17 years, 11 months ago)

I did liquid culture a week ago. I did everything right, boiled jars(250 ml-those salsa sauce jars) in water for over 30 min. Put some honey(2 or 3 teaspoons-till the color was right) in it closed the lid and boiled again for about 30 min. Then i left them to cool overnight. So when i put spores in they were completely cooled. Now aweek later i can see spores floating in honey water but no mycelium appears. I keep jars under the radiator (25˙C). So the question is why they dont grow? Is it possible that my spore prints are fucked up?? I hope not coz ill have to order new ones again:(


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?Bibere humantium est, ergo bibamus.
Drinking is human habit, so lets drink


Edited by kike (02/12/06 03:05 AM)


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OfflineDrone
Derka Derka Derka
Male

Registered: 07/22/04
Posts: 667
Loc: Maryland
Last seen: 5 years, 1 month
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: kike]
    #5290722 - 02/12/06 03:06 AM (17 years, 11 months ago)

2or3 teaspoons sounds like too much honey.

and the last LC i did took 7 days for me to see growth signs.


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Offlinekrill
absolute gonzo
Male

Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: Drone]
    #5290725 - 02/12/06 03:17 AM (17 years, 11 months ago)

i've had a similar problem with a few of my LC's.
i did a run of LC's (inoc'ed on 1/29) using 4 different strains

-Treasure Coast
-Ecuador
-TKS Super Strain
-Mexi-Cub

i used the Baby Bottle (w/ 6cc Karo syrup each) Tek

the Treasure Coast and Ecuador LC's are ready (enough-i'll wait a bit longer) to make syringes or transfer to vacutainers.

the Super Strain and Mexi-Cub LC's both have fair size chunks of spores that have fuzz growing around them, and i've been able to break a few puffs free... but that's it.
maybe certain strains take longer, maybe something caused them to die... i really don't know what's up with them 'cept i'll keep waiting to see what they do!


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"DO NOT ADJUST YOUR MIND.  IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson

NO LEFT TURN UN-STONED


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Offlinebananahands
Stranger
Registered: 01/30/06
Posts: 8
Last seen: 17 years, 10 months
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: krill]
    #5290734 - 02/12/06 03:29 AM (17 years, 11 months ago)

did ya shake it up?


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Offlinekike
Spirit chaser
Registered: 02/08/06
Posts: 4
Loc: europe
Last seen: 17 years, 9 months
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: bananahands]
    #5290737 - 02/12/06 03:34 AM (17 years, 11 months ago)

I have marble in it so i can shake them whole time but no mycelium appears


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?Bibere humantium est, ergo bibamus.
Drinking is human habit, so lets drink


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Offlinehawksapprentice
Yearns to Snowboard
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Registered: 06/06/03
Posts: 3,195
Loc: Oregon
Last seen: 8 months, 6 days
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: kike]
    #5290773 - 02/12/06 04:36 AM (17 years, 11 months ago)

I prefer to put living mycellium in it as compared to straight spores. They tend to take off alot quicker.

I have had LC inoculated with spores take up to 3 weeks to germ. under 85F. conditions. Give it some time.


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"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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OfflineBMArts
Stranger
Registered: 01/07/06
Posts: 215
Last seen: 16 years, 11 months
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: hawksapprentice]
    #5290868 - 02/12/06 06:55 AM (17 years, 11 months ago)

first time I tried LC I did a honey tek but it didn't work at all... I think due to the honey content. I think Dextrose/Malt is the best LC.
Anyhow... did you shake your syringe of spores before innoculating? Recently I forgot and nothing was growing in the jar I had innoced (it was a test jar to see how the spores were)


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Everything I post on this board is pure fiction. Nothing in the post above is real. It is all made up...


May the source be with GNU


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InvisibleHyphalTryptamin
Stranger

Registered: 05/07/05
Posts: 390
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: BMArts]
    #5290875 - 02/12/06 07:05 AM (17 years, 11 months ago)

* one * teaspoon of honey / malt syrup etc is PLENTY for 250ml. It's always safer to go less than more. If you're using powders, such as dextrose monohydrate then it's a tablespoon to 250ml.


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OfflineSinthetic
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Registered: 12/05/05
Posts: 812
Last seen: 15 years, 6 months
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: HyphalTryptamin]
    #5290982 - 02/12/06 08:59 AM (17 years, 11 months ago)

I agree. 1 tea spoon is plenty.


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OfflineChronicStiffy
Pothead
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Registered: 11/15/05
Posts: 49
Last seen: 17 years, 15 days
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: Sinthetic]
    #5290995 - 02/12/06 09:07 AM (17 years, 11 months ago)

Well, Otto's Tek : http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5238137/an/0/page/0 says to use a tablespoon of honey for 250 ml so I don't think the amount of honey would be the culprit here.

Also, I have done a tablespoon of honey in 250 ml and I have had a successful LC.  Maybe the jar should have been shook before and after inoculation.

:peace:


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Offlinekike
Spirit chaser
Registered: 02/08/06
Posts: 4
Loc: europe
Last seen: 17 years, 9 months
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: ChronicStiffy]
    #5291467 - 02/12/06 01:27 PM (17 years, 11 months ago)

This evnin i made new jars. i put 1 teaspoon of honey in it(the waters completely clear). Now i have only half spore print left so i have to be carefull for possible contam.
Do u think it is good idea to leave jars with honey water(without spores)for few days just to check that there isnt any contam in them??
then put some spores in after a week or so.


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?Bibere humantium est, ergo bibamus.
Drinking is human habit, so lets drink


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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: kike]
    #5291538 - 02/12/06 01:51 PM (17 years, 11 months ago)

What kind of honey did you use?

I think it's possible it could of had some kind of preservative in it that is inhibiting the mycelium's growth.

It can be difficult to spot contams in LC even with a trained eye.


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My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!


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OfflineNewbieS
User of semicolons.
Male User Gallery

Registered: 07/18/04
Posts: 24,710
Loc: SoCal
Last seen: 1 day, 16 hours
Re: LIQUID CULTURE DOESNT WORK?? I put spores in but the grow doesnt start!!! [Re: musher_420]
    #5291552 - 02/12/06 01:56 PM (17 years, 11 months ago)

Yeah try getting all natural honey, the clearer the better.  I put about 2 tablespoons in mine and didn't see growth for a while but now it's about a 3/4" long and 1/4" thick.  Wait another week...if nothing then try again.  15cc of spores goes a LONG way :smile:


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