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Offlinecultzombieflik
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Registered: 02/09/06
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liquid culture?
    #5288980 - 02/11/06 02:56 PM (17 years, 11 months ago)

how long can i keep a liquid culture in the jar without it dying or going bad. And what are some ways of keeping mycelium or liquid cultures for a long time.


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OfflineSnaggletooth
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Re: liquid culture? [Re: cultzombieflik]
    #5289112 - 02/11/06 03:56 PM (17 years, 11 months ago)

Mycelium can live for a long time(Months)in LC, I don't know the limit.

You can transfer them into a Vacutainer or look into making slants.


Keep them cool and in a dark place.
:mushroom2:


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Atheist Chat


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Offlinecougercruiser
reality is whatyou make it~

Registered: 10/05/04
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Re: liquid culture? [Re: Snaggletooth]
    #5289153 - 02/11/06 04:04 PM (17 years, 11 months ago)

spores can live up to two years inside of syring with distilled water~


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Offlinespooky
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Registered: 10/04/05
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Re: liquid culture? [Re: cougercruiser]
    #5289597 - 02/11/06 06:11 PM (17 years, 11 months ago)

also, isnt the amount of time it will stay alive in LC proportionate to amount of food ya give em (like honey)? more food = they stay alive longer, or am i wrong ?


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OfflineSnaggletooth
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Re: liquid culture? [Re: spooky]
    #5289604 - 02/11/06 06:15 PM (17 years, 11 months ago)

Not relay, case you can use distilled water that would have not nuts in it

They kinda fall asleep, hibernate, growth stalls


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Atheist Chat


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InvisibleFooMan
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Re: liquid culture? [Re: cultzombieflik]
    #5289613 - 02/11/06 06:20 PM (17 years, 11 months ago)

Put it in the fridge when it's done growing.


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Quick WBS Prep


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OfflineEquilibriuM
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Re: liquid culture? [Re: FooMan]
    #5289618 - 02/11/06 06:22 PM (17 years, 11 months ago)

after 4-6 months in the fridge they seem much more prone to fall victim to contamination.


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HELP!!!!!!!!!


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OfflineSnaggletooth
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Re: liquid culture? [Re: EquilibriuM]
    #5289621 - 02/11/06 06:23 PM (17 years, 11 months ago)

No, not if you have it sealed like in a vacutainers


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OfflineEquilibriuM
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Re: liquid culture? [Re: Snaggletooth]
    #5289792 - 02/11/06 07:34 PM (17 years, 11 months ago)

well in my experience they do. not in vacutainers but in jars and they colonize sterile grain just fine but when the grain is then exposed to h/poo they contaminate with mold about 50% of the time whereas fresh (only a month or 2 old) LC under the same conditions contaminates with h/poo about 4-8% of the time.


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Offlinecougercruiser
reality is whatyou make it~

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Re: liquid culture? [Re: EquilibriuM]
    #5289932 - 02/11/06 08:16 PM (17 years, 11 months ago)

well to prove all ur theorys true or false i am conducting an experament with three filled syringes that i had sitting in my closet for 11 months. i am going to enject them into wbs jars that i have inoculated with each jar one with amazonian, another with ecuadorian, the third with treaser coast and the last with ecuadorian and amazonian....its been two days so far.....i will cheack on thursday pacific time to see if anything happens....it should work.....everything was shipped to me and sterilized by a lab....so no contams~ =)


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InvisibleJaeger
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Re: liquid culture? *DELETED* [Re: cougercruiser]
    #5289944 - 02/11/06 08:20 PM (17 years, 11 months ago)

Post deleted by Jaeger

Reason for deletion: .



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Offlinecougercruiser
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Re: liquid culture? [Re: Jaeger]
    #5290059 - 02/11/06 08:54 PM (17 years, 11 months ago)

I DUNNO BUT IMO I WOULDNT THINK SO~


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InvisibleFooMan
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Re: liquid culture? [Re: cougercruiser]
    #5290070 - 02/11/06 09:06 PM (17 years, 11 months ago)

Quote:

cougercruiser said:
well to prove all ur theorys true or false i am conducting an experament with three filled syringes that i had sitting in my closet for 11 months. i am going to enject them into wbs jars that i have inoculated with each jar one with amazonian, another with ecuadorian, the third with treaser coast and the last with ecuadorian and amazonian....its been two days so far.....i will cheack on thursday pacific time to see if anything happens....it should work.....everything was shipped to me and sterilized by a lab....so no contams~ =)




Are you talking about spores or an LC? I don't know of any "lab" that will mail you live cultures, the topic of this thread.


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OfflineEquilibriuM
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Re: liquid culture? [Re: FooMan]
    #5290250 - 02/11/06 10:14 PM (17 years, 11 months ago)

yeah, spores should work fine aafter a year...


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Offlinecougercruiser
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Re: liquid culture? [Re: EquilibriuM]
    #5290295 - 02/11/06 10:33 PM (17 years, 11 months ago)

not lab, mycomonster works out of a lab.....sent me sterilized everything......spores where sittin inside of a sterilized syring filled with distilled water in the closet that i got from sporemagic.com thats been sitting in closet.


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OfflineEquilibriuM
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Re: liquid culture? [Re: cougercruiser]
    #5290299 - 02/11/06 10:35 PM (17 years, 11 months ago)

Quote:

cougercruiser said:
not lab, mycomonster works out of a lab.....sent me sterilized everything......spores where sittin inside of a sterilized syring filled with distilled water in the closet that i got from sporemagic.com thats been sitting in closet.



thats not a liquid culture and should work fine.


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Offlinecougercruiser
reality is whatyou make it~

Registered: 10/05/04
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Re: liquid culture? [Re: EquilibriuM]
    #5290307 - 02/11/06 10:38 PM (17 years, 11 months ago)

ohhh sorry too bother~


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OfflineMycophreek423
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Re: liquid culture? [Re: cougercruiser]
    #5290736 - 02/12/06 03:31 AM (17 years, 11 months ago)

i have 6 year old spore syringe germ fine


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I Thought You Where There , But you Where not. It Mushed Have Been Somthing I Ate.


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Invisibletahoe
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Re: liquid culture? [Re: Mycophreek423]
    #6318766 - 11/29/06 01:02 PM (17 years, 2 months ago)

i had a lc last for 4 years. I just opened it and started over. I used it 6 months ago for the last time.

Here is the chunck of mycelium that is left over
its kind of gross looking


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


Edited by tahoe (11/29/06 01:03 PM)


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InvisibleBlutjager
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Re: liquid culture? [Re: tahoe]
    #6318778 - 11/29/06 01:14 PM (17 years, 2 months ago)

Quote:

tahoe said:
i had a lc last for 4 years. I just opened it and started over. I used it 6 months ago for the last time.

Here is the chunck of mycelium that is left over
its kind of gross looking





I'm not sure what I would do but I would come up with some strange experiment to do with all that mycelium :hehehe:

What was that LC made of,I have made some think LC but I don't think it ever had that much mycelium in it ??


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