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Offlinesoreal
Stranger
Registered: 05/30/05
Posts: 19
Last seen: 17 years, 8 months
what am i doing wrong?
    #5286633 - 02/10/06 05:02 PM (17 years, 11 months ago)

im getting that green mold in about half of all my jars. im using rye berries and doing grain transfers. im doing the transfers in a room that has a flow hood running and im spraying my self with lysol and before entering the room. im using the filter discs from paul stamits site.

do the dics need to be sprayed with lysol? any help would be great. thanks.


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Offlineshroomsynergy
Fungi Freak
Male

Registered: 01/17/06
Posts: 861
Loc: Andromeda
Last seen: 4 years, 4 months
Re: what am i doing wrong? [Re: soreal]
    #5286668 - 02/10/06 05:11 PM (17 years, 11 months ago)

i dont think it would hurt to spray the discs, specially if there old. just check and double check for any descrepencies in your sterilization, and check for the weakest link in your steps to find were you might be lax or when in your proccess the contams are creeping in. My guess is that your getting contams during the grain transfers since this is when they seem the most prone to contams. other than that i dunno...maybe agar or snagletooth or carlos have some good ideas for you.


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Edited by ShrOOmSynEgry (02/10/06 05:12 PM)


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Offlineonetime
onetime
 User Gallery

Registered: 11/13/03
Posts: 3,609
Last seen: 13 years, 1 month
Re: what am i doing wrong? [Re: soreal]
    #5286672 - 02/10/06 05:12 PM (17 years, 11 months ago)

do you do it directly in front of the flow hood?

how long do you let your hood run?

do you have one of these http://store.pksafety.net/stancov.html and one of these http://www.alerg.com/page/A/PROD/NEW/MSK1000?

Do you spray the air with oust befor you start?

how long do you pressure cook your grains for?

Do you let your grains soak befor you pressure cook?

Do you have the lid on like this Lid filter ring or filter lid ring?

some times puddles can get between the filter and lid. this is why i took agars adivce and used only micro pore tape found at any pharmacy.


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See?
Yes, with my own three eyes.
Depression, Misspells , wanting everying thing i cant have haveing nothing i want


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Offlinesoreal
Stranger
Registered: 05/30/05
Posts: 19
Last seen: 17 years, 8 months
Re: what am i doing wrong? [Re: onetime]
    #5286793 - 02/10/06 06:11 PM (17 years, 11 months ago)

im doing the transfer right in front of the flow hood, and letting it run for about an hour before i go in there. i also spray the room with lysol about 5 to 10 mins before the transfers.

i dont have a tyvek suit but i do wear and mask over my face.

i do NOT soak the berries bfore i PC them, and im PCing for 1hr15mins at 15psi

my jars go filter, lid, ring.


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Offlinegourmetgrower
I'ze the milkmanwhat knocked upyer maw

Registered: 01/29/06 Happy 18th Shroomiversary!
Posts: 170
Loc: The Old West
Last seen: 17 years, 10 months
Re: what am i doing wrong? [Re: soreal]
    #5286818 - 02/10/06 06:21 PM (17 years, 11 months ago)

--> http://www.alerg.com/page/A/PROD/NEW/MSK1000?

I like how the mask shown by clicking that link has an unfiltered output vent so that you exhale bacteria all over your cultures (the little circle with the 3M logo in it)  :biggrin:


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Howdy, boys! Let's get down to business. I got my colt and my schofield, and they's jist itchin to be broken in.


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OfflineEquilibriuM
dream stalker

Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 7 months
Re: what am i doing wrong? [Re: soreal]
    #5286820 - 02/10/06 06:21 PM (17 years, 11 months ago)

you should soak your grains overnight before PCing them.


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HELP!!!!!!!!!


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Offlinesoreal
Stranger
Registered: 05/30/05
Posts: 19
Last seen: 17 years, 8 months
Re: what am i doing wrong? [Re: EquilibriuM]
    #5287390 - 02/10/06 09:39 PM (17 years, 11 months ago)

what does soaking them do?


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Offlinegourmetgrower
I'ze the milkmanwhat knocked upyer maw

Registered: 01/29/06 Happy 18th Shroomiversary!
Posts: 170
Loc: The Old West
Last seen: 17 years, 10 months
Re: what am i doing wrong? [Re: soreal]
    #5287510 - 02/10/06 10:22 PM (17 years, 11 months ago)

soaking the grains does two things:

1st, it establishes a good balance of water, and makes it easier than wondering how much water to add to dry grain.

2nd, it causes mold spores and bacterial endospores to sprout, and since the germinated spores no longer have their thick cell walls, they are more easily KILLED!!!

KILL CONTAMINATION, MOTHERF***ERS!


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Howdy, boys! Let's get down to business. I got my colt and my schofield, and they's jist itchin to be broken in.


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OfflineBataviaVakereli
Stranger
Registered: 08/22/05
Posts: 274
Last seen: 13 years, 9 months
Re: what am i doing wrong? [Re: soreal]
    #5287513 - 02/10/06 10:23 PM (17 years, 11 months ago)

Quote:

soreal said:
what does soaking them do?




It allows endospores that can survive pressure cooking to germinate and thus allow them to be killed during the pressure cooking.


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Offlinegourmetgrower
I'ze the milkmanwhat knocked upyer maw

Registered: 01/29/06 Happy 18th Shroomiversary!
Posts: 170
Loc: The Old West
Last seen: 17 years, 10 months
Re: what am i doing wrong? [Re: BataviaVakereli]
    #5287531 - 02/10/06 10:31 PM (17 years, 11 months ago)

That's what I just said. But I added vulgarity, to make it more interesting.  :gethigh:


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Howdy, boys! Let's get down to business. I got my colt and my schofield, and they's jist itchin to be broken in.


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Offlinesoreal
Stranger
Registered: 05/30/05
Posts: 19
Last seen: 17 years, 8 months
Re: what am i doing wrong? [Re: gourmetgrower]
    #5287569 - 02/10/06 10:56 PM (17 years, 11 months ago)

anythingelse that could be causing my mold prob?


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OfflineKeen_Eyed
Keen_Eyed

Registered: 01/23/06
Posts: 61
Loc: Arizona, USA
Last seen: 17 years, 10 months
Re: what am i doing wrong? [Re: soreal]
    #5287724 - 02/11/06 12:35 AM (17 years, 11 months ago)

there could be mold falling from your hair. I had that problem on my first try and then took a chance that it was my hair and cut it off and no more contam. problem. and then I grew it back out again and then I had the same problem starting all over again. And the second thing is, Lysol is a great thing but you cannot depend on that to kill all the grems and bact. in the air. you need to mix a rubbing alcohol, distilled water mix in a spray bottle at a ratio of 15% alcohol and the rest warm water. Spray the air and your hair your hands your clothes, your shoes, anything that you can think to spray, more you spray the better the chances are that you wont get contam's. You might not have had that batch of jars sterilized good enough. There are just too many factors.


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U & I, Well Go Get Keen Eyed!


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OfflineKeen_Eyed
Keen_Eyed

Registered: 01/23/06
Posts: 61
Loc: Arizona, USA
Last seen: 17 years, 10 months
Re: what am i doing wrong? [Re: Keen_Eyed]
    #5287729 - 02/11/06 12:38 AM (17 years, 11 months ago)

you could even go straight rubbing alcohol in the spray bottle.


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U & I, Well Go Get Keen Eyed!


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OfflineDoctor
Strangled
Male User Gallery

Folding@home Statistics
Registered: 03/05/05
Posts: 155
Last seen: 9 years, 6 months
Re: what am i doing wrong? [Re: Keen_Eyed]
    #5287812 - 02/11/06 01:33 AM (17 years, 11 months ago)

Quote:

Keen_Eyed said:
there could be mold falling from your hair. I had that problem on my first try and then took a chance that it was my hair and cut it off



:shocked: you could of tied it back or covered it, hardcore.  :laugh:


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Offlinesoreal
Stranger
Registered: 05/30/05
Posts: 19
Last seen: 17 years, 8 months
Re: what am i doing wrong? [Re: Doctor]
    #5289026 - 02/11/06 03:22 PM (17 years, 11 months ago)

ill try covering my hair and using the alcohol


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