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Psychonaught
Preformer of Mycophagia

Registered: 10/21/05
Posts: 368
Loc: Traveler if space and tim...
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How much heat can a spore print take?
#5284351 - 02/10/06 01:01 AM (17 years, 11 months ago) |
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I hear a syringe is good to 110 degrees Fahrenheit, What about a print in foil?
Mr. X refuses to build a desiccant chamber for a print. The plan is to Place the foiled print with three of four sides folded, in a ronco food-dehydrator to get the prints all nice n' dry before storage?
How long can a print stay viable in a cool, dry environment while in foil?
-------------------- "How could this poison be the dream of my soul...I let my fantasy take complete control." -Black Sabbath "megalomania" *************** Double Tub Tek Pictorial
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Sinthetic
Stranger


Registered: 12/05/05
Posts: 812
Last seen: 15 years, 6 months
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Re: How much heat can a spore print take? [Re: Psychonaught]
#5284705 - 02/10/06 05:56 AM (17 years, 11 months ago) |
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That will be fine.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: How much heat can a spore print take? [Re: Psychonaught]
#5284752 - 02/10/06 06:24 AM (17 years, 11 months ago) |
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Exposing a print (or any part of it) to OPEN AIR, will dramatical increase the chance of it becoming contaminated.
I don't own, nor have experience with a Ronco dehydrator. But, as I understand them. They operate in the 145 / 165F range. And, do not contain a fan of any sort.
So, air movement inside them is caused by heat CONVECTION.
I would not intentionally expose a print to those temperature ranges, or circumstance & retain good faith in their full viability, or complete sterility.
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Psychonaught
Preformer of Mycophagia

Registered: 10/21/05
Posts: 368
Loc: Traveler if space and tim...
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Re: How much heat can a spore print take? [Re: agar]
#5285004 - 02/10/06 08:56 AM (17 years, 11 months ago) |
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Acctually AGAR, the ronco is rated at 140 degrees and with 7 trays the top tray stays at 104 degrees.
-------------------- "How could this poison be the dream of my soul...I let my fantasy take complete control." -Black Sabbath "megalomania" *************** Double Tub Tek Pictorial
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_OttO_
Over Stimulated


Registered: 06/01/05
Posts: 2,588
Loc: Up Over
Last seen: 1 month, 12 days
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Re: How much heat can a spore print take? [Re: Psychonaught]
#5285266 - 02/10/06 10:11 AM (17 years, 11 months ago) |
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Man I have no idea why the hell you would want to put your prints in a dehydrator before storing them. This is just silly IMO.
Just take the cap off the foil after its dropped its spores, fold up and store - if you want to be sure its dry, take the cap off, leave the print OPEN but COVERED with a glass tumbler for 24 hours, and then just seal and store your print.
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Omnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: How much heat can a spore print take? [Re: _OttO_]
#5285371 - 02/10/06 10:37 AM (17 years, 11 months ago) |
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^^^^
no need for the dyhydrator
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: How much heat can a spore print take? [Re: Omnicracker]
#5285420 - 02/10/06 10:56 AM (17 years, 11 months ago) |
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Psychonaught
Preformer of Mycophagia

Registered: 10/21/05
Posts: 368
Loc: Traveler if space and tim...
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Re: How much heat can a spore print take? [Re: agar]
#5285585 - 02/10/06 11:44 AM (17 years, 11 months ago) |
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From what I gathered from the printing tek this step was necessary, I suppose a shoe box with some desiccant packets would work.
Does this extra drying really even need to be done at all?
-------------------- "How could this poison be the dream of my soul...I let my fantasy take complete control." -Black Sabbath "megalomania" *************** Double Tub Tek Pictorial
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Psychonaught
Preformer of Mycophagia

Registered: 10/21/05
Posts: 368
Loc: Traveler if space and tim...
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Re: How much heat can a spore print take? [Re: Psychonaught]
#5285590 - 02/10/06 11:46 AM (17 years, 11 months ago) |
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Ok NM, I'll stop beating this dead horse before it turns to pudding.
-------------------- "How could this poison be the dream of my soul...I let my fantasy take complete control." -Black Sabbath "megalomania" *************** Double Tub Tek Pictorial
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: How much heat can a spore print take? [Re: Psychonaught]
#5285593 - 02/10/06 11:47 AM (17 years, 11 months ago) |
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Spores can handle up to 122F (50C) without problems whether in a syringe or on a print. This is the temp where the spores begin to deteriorate it's the duration at these temps that will determine the viability of the spores.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: How much heat can a spore print take? [Re: Psychonaught]
#5285713 - 02/10/06 12:27 PM (17 years, 11 months ago) |
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You should allow a print to dry. Because excess moisture will give rise to bacterial growth.  Possibly even partial spore gemination. 
Dry prints store well.  Moist prints DO NOT. 
After removing caps, in a G/B. I cover the trays with loose sanitized A/foil - at least 24 hours. That does the trick. (at least for me).
 
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