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BMArts
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Registered: 01/07/06
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How will it effect the strain by 'tweaking' it to your home parameters?
#5280641 - 02/09/06 03:33 AM (18 years, 3 months ago) |
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I've read somewhere that if you clone a mush that did very well in your environment chances are you can start cultivating your strains to be ones that are trimmed to your own environment. Perhaps this even works without cloaning but using spores? Anyway... so say I from now on always use the same mixture LC and always use Rye from the same store preparing it in the same method, etc. Eventually after a few generations will the strain I'm working with slowly tweak to the conditions I'm growing with? (would they start performing better on Rye and if introduced to another substrate not do so good?) What are the pros and cons? And if you wanted to activly support this kind of process how would one go about it? Thanks guys
-------------------- Everything I post on this board is pure fiction. Nothing in the post above is real. It is all made up...
May the source be with GNU
Edited by BMArts (02/09/06 03:34 AM)
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ohmatic
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Registered: 02/28/04
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Loc: europe
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Re: How will it effect the strain by 'tweaking' it to your home parameters? [Re: BMArts]
#5280667 - 02/09/06 03:58 AM (18 years, 3 months ago) |
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Quote:
BMArts said: Perhaps this even works without cloaning but using spores?
no. peace ohm
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MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
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DocPsilocybin
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Re: How will it effect the strain by 'tweaking' it to your home parameters? [Re: ohmatic]
#5282195 - 02/09/06 03:10 PM (18 years, 3 months ago) |
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It's not quite that simple. You can try to isolate certain characteristics by cloning but as soon as you go try to use the spores these mushrooms produce you're back at square one. Genetics at play. If you keep on cloning off your parent stock eventually the clones stop doing so well and you need to make a new cloning stock.
Simplistic explanation for now. If you don't understand the genetics I can explain them after class.
-------------------- You can't hold a man down without staying down with him. -- Booker T. Washington
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FungusMan
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Re: How will it effect the strain by 'tweaking' it to your home parameters? [Re: DocPsilocybin]
#5282785 - 02/09/06 06:05 PM (18 years, 3 months ago) |
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Yup. If you keep using the same line, over time and generations, you will get more mutants. Mutants can be cool sometimes,lol.
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Sinthetic
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Re: How will it effect the strain by 'tweaking' it to your home parameters? [Re: FungusMan]
#5284967 - 02/10/06 08:42 AM (18 years, 3 months ago) |
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Here is the best way to accomplish what you want.
1. Grow some shrooms. 2. Take the best looking mushy and clone it to some Agar. 3. Take a chunk of the best looking myc and use it to inoculate. 4. repeat steps 1-3. 5. Once you have a strain you like clone it to agar and put it into a freezer bag and into the fridge. This will stay good for months. whenever you want to start a grow. take a piece of myc out of the fridge and use it to inoculate.
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blackout


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Re: How will it effect the strain by 'tweaking' it to your home parameters? [Re: Sinthetic]
#5285083 - 02/10/06 09:18 AM (18 years, 3 months ago) |
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Some people like to add their end substrate to the starting agar plates, seems to make sense that strains that do well on a rye agar will do well on rye grain. I always thought if you wanted a strain that did well at lower temps you would do a MS and leave it at a lower temp, the "winning" strain should be tolerant of cold, the other strains showing weaker growth. Therefore I would expect the clone of the cold fruiting substrate to also do well in colder settings. But I would not expect spores from these clones to be "cold loving spores".
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cultzombieflik
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Re: How will it effect the strain by 'tweaking' it to your home parameters? [Re: blackout]
#5287341 - 02/10/06 09:24 PM (18 years, 3 months ago) |
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It is deffinately possible to "tweak" the mushrooms, have you ever heard of natural selection. Darwins theory of natural selection states that oranisms with natural characteristics that help them survive in their environment will have more of an opportunity to reproduce and therefore will produce more "offspring" like themselves and the organisms without those traits wont be able to handle the competition and will eventually be weeded out and the whole species will change. that is how evolution happens. what you are talking about is just forcing this to happen immediately "unnatural selection" Sorry didnt mean to give a science lesson, got carried away.
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Cyano
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Re: How will it effect the strain by 'tweaking' it to your home parameters? [Re: cultzombieflik]
#5287861 - 02/11/06 02:44 AM (18 years, 3 months ago) |
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If you want a strain to adapt to your specific growing conditions, don?t make clones. Just use spores from the best looking mushrooms. Spores have genetic variation. Variation is necessary for adaption.
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BMArts
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Re: How will it effect the strain by 'tweaking' it to your home parameters? [Re: Cyano]
#5288034 - 02/11/06 07:32 AM (18 years, 3 months ago) |
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hmm well some ppl say spores, some say clonse... its interesting either way. I dunno
-------------------- Everything I post on this board is pure fiction. Nothing in the post above is real. It is all made up...
May the source be with GNU
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shroomydan
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Registered: 07/04/04
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Re: How will it effect the strain by 'tweaking' it to your home parameters? [Re: BMArts]
#5288146 - 02/11/06 09:02 AM (18 years, 3 months ago) |
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It seems to me that if you are trying to find a strain which is well adapted to your environment, you should go with spores. Once you find one that works, then you need to clone it. The benefit of clones is that they are stable. Spores give variation, and as Darwin noted, variation is necessary for evolution.
So yeah, if you are attempting to find a strain which can thrive in your environment, you should go with spores; once you find one that is excellent, then clone it.
I am curious about something though. When working with a clone, if one were interested in promoting a specific characteristic, say large size fruits, would it be beneficial to always use spores from the biggest fruit, or are the same genetics available in the spores of any mushroom from a given colony?
Intuitively it seems obvious that one should chose the bigger fruit, but it seems to me that genetics are uniform within a colony of mycelium, and that fruit size is due to environmental conditions.
Does anybody know about this?
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Cyano
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Re: How will it effect the strain by 'tweaking' it to your home parameters? [Re: shroomydan]
#5290684 - 02/12/06 01:51 AM (18 years, 3 months ago) |
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When using clones the same genetics should be available in the spores of any mushroom from a given colony. In that case fruit size is due to environmental conditions.
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