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rygo796
Meat Popsicle


Registered: 04/17/05
Posts: 221
Last seen: 15 years, 6 months
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Used syringe Q.
#5278016 - 02/08/06 03:25 PM (17 years, 11 months ago) |
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I got some syringes i wanna use for my LC...all they were previously used for was depositing spores into jars. Can I just suck some boiling water into em of do they need .to be PC'd?
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License_to_Chill
Stranger


Registered: 11/12/04
Posts: 4,979
Last seen: 3 years, 2 months
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Re: Used syringe Q. [Re: rygo796]
#5278030 - 02/08/06 03:26 PM (17 years, 11 months ago) |
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I heard you can suck up some boiling water and spray it out into the sink. Do this about 4 or 5 times and them flame sterilize the needle. Should be sterile then.
Now, I've never tried this, but heard it works. Good luck.
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"I sat on the bus next to God once, he told me about the true meaning of life... then he gave me a pretzel..."
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Used syringe Q. [Re: rygo796]
#5278051 - 02/08/06 03:31 PM (17 years, 11 months ago) |
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PC, to be positive.
Other methods works sometimes.
Some even work most of the time.
PC'ing works EVERY TIME.
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License_to_Chill
Stranger


Registered: 11/12/04
Posts: 4,979
Last seen: 3 years, 2 months
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Re: Used syringe Q. [Re: agar]
#5278098 - 02/08/06 03:40 PM (17 years, 11 months ago) |
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Be sure to wrap it in aluminum foil, first.
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"I sat on the bus next to God once, he told me about the true meaning of life... then he gave me a pretzel..."
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Wronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
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I routinely use boiling water just before I suck up liquid culture and in between cultures. I have done it without any problems. Agar is right however; pressure cooking is the only sure method.
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Used syringe Q. [Re: rygo796]
#5278508 - 02/08/06 05:33 PM (17 years, 11 months ago) |
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just one caution...
some syringes can't withstand being pressure cooked.
the boiling water method talked about above...has never let me down.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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joker_66599
NOOB / SMALLTIME GROWER


Registered: 07/18/05
Posts: 758
Last seen: 3 years, 11 months
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Re: Used syringe Q. [Re: Roadkill]
#5278555 - 02/08/06 05:52 PM (17 years, 11 months ago) |
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ooo shat lol just pc my 2 syringes i have for my LC hope they lived that thats true
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?¿ ¿? I don't really grow mushrooms I just act like it to be coo. ?¿ ¿?
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joker_66599
NOOB / SMALLTIME GROWER


Registered: 07/18/05
Posts: 758
Last seen: 3 years, 11 months
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sweet still alive only oc on high for 16 mins shouls i have done it longer?
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?¿ ¿? I don't really grow mushrooms I just act like it to be coo. ?¿ ¿?
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rawtoxic
Stranger
Registered: 10/06/02
Posts: 2,097
Loc: smokey mountains
Last seen: 13 years, 5 months
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that should work fine.
i usually PC my next batch of LCs and empty syringes together for 15-20 mins.
since you have to PC the LC 15 mins this is a good time to go ahead and sterlize syringes.
Edited by rawtoxic (02/08/06 09:52 PM)
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mky
MadMan

Registered: 07/10/05
Posts: 51
Loc: If you know tell me
Last seen: 15 years, 3 months
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Re: Used syringe Q. [Re: rawtoxic]
#5281121 - 02/09/06 09:45 AM (17 years, 11 months ago) |
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I just suck up some boiling water and squirt it out about 3 times and then go straight to the LC and suck it up. No flaming needle so far so good, no contams.
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thenewguy05
The Mushroom Man


Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
Last seen: 2 years, 5 months
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Re: Used syringe Q. [Re: mky]
#5281145 - 02/09/06 09:53 AM (17 years, 11 months ago) |
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I've used this method of sucking up boiling water i usually squirt it out a few times and then leave it in there for about 5-10 minutes to keep the heat in and the spray it out as usuall. then for the last time suck it up and leave it in there again. heat is a spores worst enemy so i try to expose it to the heat as long as possible.
agar is right. pressure cooking works everytime!
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Gopal
Strange

Registered: 07/09/04
Posts: 65
Last seen: 8 years, 3 months
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Do you think that hydrogen peroxide could be used in the same way as the boiling water to sterilize the syringes?
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liloldme
( ͝° ͜ʖ͡°)つ=D


Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Used syringe Q. [Re: Gopal]
#5303254 - 02/15/06 01:47 PM (17 years, 11 months ago) |
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Ok this is what I do everytime, it has worked everytime, except once, because i pc'ed them for more than 15-20 min, and they squirted out half the water in them. Just put freash water in them, wrap each one w/ enough tin foil to keep them from blowing open, ie piston blowing outta the cylinder, and pc them wraped in a towel for like 15 min, let them depressurize as normal(slowly). Then they are good to go! I have reused the same 10 syringes doing it this way like ten times, they dont look new but they work great.
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fungiamongi2
pharmer



Registered: 03/10/05
Posts: 1,042
Last seen: 10 years, 9 months
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Re: Used syringe Q. [Re: Gopal]
#5303260 - 02/15/06 01:50 PM (17 years, 11 months ago) |
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not sure, but i know ive always been good with sucking them full of alcohol, then water
-------------------- gonna bring her a kiss, make those blues run
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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i have use'd the boiling water method....
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Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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