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Offlinerygo796
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Registered: 04/17/05
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Used syringe Q.
    #5278016 - 02/08/06 03:25 PM (17 years, 11 months ago)

I got some syringes i wanna use for my LC...all they were previously used for was depositing spores into jars. Can I just suck some boiling water into em of do they need .to be PC'd?


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OfflineLicense_to_Chill
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Re: Used syringe Q. [Re: rygo796]
    #5278030 - 02/08/06 03:26 PM (17 years, 11 months ago)

I heard you can suck up some boiling water and spray it out into the sink.  Do this about 4 or 5 times and them flame sterilize the needle.  Should be sterile then.

Now, I've never tried this, but heard it works.  Good luck. :sun:


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"I sat on the bus next to God once,
he told me about the true meaning of life...
then he gave me a pretzel..."


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Invisibleagar
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Re: Used syringe Q. [Re: rygo796]
    #5278051 - 02/08/06 03:31 PM (17 years, 11 months ago)

PC, to be positive.

Other methods works sometimes.

Some even work most of the time.

PC'ing works EVERY TIME.


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OfflineLicense_to_Chill
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Re: Used syringe Q. [Re: agar]
    #5278098 - 02/08/06 03:40 PM (17 years, 11 months ago)

Be sure to wrap it in aluminum foil, first. :smile:


--------------------
"I sat on the bus next to God once,
he told me about the true meaning of life...
then he gave me a pretzel..."


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OfflineWronguy
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Re: Used syringe Q. [Re: License_to_Chill]
    #5278377 - 02/08/06 04:47 PM (17 years, 11 months ago)

I routinely use boiling water just before I suck up liquid culture and in between cultures. I have done it without any problems. Agar is right however; pressure cooking is the only sure method.


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InvisibleRoadkillM
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Re: Used syringe Q. [Re: rygo796]
    #5278508 - 02/08/06 05:33 PM (17 years, 11 months ago)

just one caution...

some syringes can't withstand being pressure cooked.


the boiling water method talked about above...has never let me down.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinejoker_66599
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Re: Used syringe Q. [Re: Roadkill]
    #5278555 - 02/08/06 05:52 PM (17 years, 11 months ago)

ooo shat lol just pc my 2 syringes i have for my LC hope they lived that thats true


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?¿
¿?
I don't really grow mushrooms I just act like it to be coo.
?¿
¿?


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Offlinejoker_66599
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Re: Used syringe Q. [Re: joker_66599]
    #5278568 - 02/08/06 05:58 PM (17 years, 11 months ago)

sweet still alive only oc on high for 16 mins shouls i have done it longer?


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?¿
¿?
I don't really grow mushrooms I just act like it to be coo.
?¿
¿?


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Offlinerawtoxic
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Re: Used syringe Q. [Re: joker_66599]
    #5279557 - 02/08/06 09:51 PM (17 years, 11 months ago)

that should work fine.

i usually PC my next batch of LCs and empty syringes together for 15-20 mins.

since you have to PC the LC 15 mins this is a good time to go ahead and sterlize syringes.


Edited by rawtoxic (02/08/06 09:52 PM)


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Offlinemky
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Re: Used syringe Q. [Re: rawtoxic]
    #5281121 - 02/09/06 09:45 AM (17 years, 11 months ago)

I just suck up some boiling water and squirt it out about 3 times and then go straight to the LC and suck it up. No flaming needle so far so good, no contams.


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Offlinethenewguy05
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Re: Used syringe Q. [Re: mky]
    #5281145 - 02/09/06 09:53 AM (17 years, 11 months ago)

I've used this method of sucking up boiling water i usually squirt it out a few times and then leave it in there for about 5-10 minutes to keep the heat in and the spray it out as usuall. then for the last time suck it up and leave it in there again. heat is a spores worst enemy so i try to expose it to the heat as long as possible.

agar is right. pressure cooking works everytime!


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OfflineGopal
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Re: Used syringe Q. [Re: thenewguy05]
    #5303210 - 02/15/06 01:37 PM (17 years, 11 months ago)

Do you think that hydrogen peroxide could be used in the same way as the boiling water to sterilize the syringes?


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InvisibleliloldmeFacebook
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Re: Used syringe Q. [Re: Gopal]
    #5303254 - 02/15/06 01:47 PM (17 years, 11 months ago)

Ok this is what I do everytime, it has worked everytime, except once, because i pc'ed them for more than 15-20 min, and they squirted out half the water in them. Just put freash water in them, wrap each one w/ enough tin foil to keep them from blowing open, ie piston blowing outta the cylinder, and pc them wraped in a towel for like 15 min, let them depressurize as normal(slowly). Then they are good to go! I have reused the same 10 syringes doing it this way like ten times, they dont look new but they work great.


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Offlinefungiamongi2
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Re: Used syringe Q. [Re: Gopal]
    #5303260 - 02/15/06 01:50 PM (17 years, 11 months ago)

not sure, but i know ive always been good with sucking them full of alcohol, then water


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gonna bring her a kiss, make those blues run


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OfflineOatman2000
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Re: Used syringe Q. [Re: fungiamongi2]
    #5303339 - 02/15/06 02:15 PM (17 years, 11 months ago)

i have use'd the boiling water method....


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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