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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Prints in squat wide-mouth glass jars. 6
#5276177 - 02/08/06 02:36 AM (17 years, 11 months ago) |
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Nothing NEW here. Just advise for new hands, who don't know.
Ball has a 250 ml SQUAT wide mouth canning jar out.
  
  
 
 
Simply drill a hole in the lid & install an injection port.
If you don't have an injection port. Drill an 1/8th inch hole in the lid. Scuff the surface, around the hole. (both sides, so sealant sticks better)
Dab on some RTV silicone high temp gasket sealer over the hole. (both sides). Allow to cure at least overnight.
PC jar 20 minutes @ 15 psi, with lid LOOSE. (sterilizes it) Remove from PC hot & tighten lid.
In a glove box, or under a flow hood. Cleanly severe a a mushroom cap, place in print jar. Leave lid on 3/4 turn LOOSE (gas exchange - so you don't get condensation from a wet cap).
Allow print to set 24 (+/-)hours. In glove box, carefully remove cap & replace lid. Leave lid 3/4 turn LOOSE for another 24 hours (place in mild indirect sunlight, so the print dries).
Tighten lid TIGHT.
Knock/rap jar on semi/hard surface to dislodge spores.
With a large capacity syringe. (or whatever syringe size you have)
 Inject 125 / 200 ml of STERILE water. Shake jar violently.
Hint - put a few drops of Jet Dry in the STERILE water.
Hint - to make STERILE WATER. simply PC another jar (as above). With STERILE water & Jet Dry solution in it. (Jet Dry serves as an anti-clumping agent) (hint - distilled water works best)
There you have it. Enough spore solution to fill a dozen + 10 ml syringes.
For those with an ultrasonic cleaner.
 Before making new spore syringes. Place sealed jar in it, for an hour. (disperses spores PERFECTLY, so all syringes are fairly equal)
Hint, once print jar is almost EMPTY. Inject 150 ml of sterilized nutrient solution, to make LC.  ~~~~~~~~~~~~~~~~ EDIT TO ADD A VARIANT GAS EXCHANGE METHOD
 
For better gas exchange. Cut a small piece of tyvek to cover the lid. Place tyvek over the jar mouth. Screw lid on TIGHT, to form the tyvek to lid shape. Use a razor, or Exacto knife to trim off excess tyvek.
PC the jar, with the tyvek lid in place. In glove box, cleanly sever mushroom cap. Remove jar ring & tyvek. Place mushroom cap in jar. Replace tyvek & jar ring. Screw ring on TIGHT.
Allow 24-36 hours for print to set thickly. Remove cap, replace tyvek & jar ring, screw ring on tightly. Allow 24 hours for print to DRY.
Remove tyvek, replace with sanitized injection port lid.

The object of this variant is: 1 > to allow for optimal gas exchange. 2 > So, the cap doesn't begin to rot. 3 > so the print dries quickly.
EDIT (again)
Do not try to pound big caps into little jars.

Edited by agar (02/13/06 07:18 AM)
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
#5276191 - 02/08/06 02:53 AM (17 years, 11 months ago) |
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Another wonderful little write-up. That's a great idea to turn it into an LC jar once it's nearly cashed too. Good job 
Where do you get the injection ports from?
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
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Re: Prints in squat wide-mouth glass jars. [Re: tiny_rabid_birds]
#5276198 - 02/08/06 02:58 AM (17 years, 11 months ago) |
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Good write up. I use those shallow glass salsa dip jars like that.
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kiss
numbnuts


Registered: 12/14/04
Posts: 486
Loc: under your bed
Last seen: 9 years, 1 month
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Re: Prints in squat wide-mouth glass jars. [Re: blackout]
#5276326 - 02/08/06 05:16 AM (17 years, 11 months ago) |
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Thats the jars I was talking about Agar. Mine are abit smaller though. Almost the size of baby food jars.
-------------------- see dick drink see dick drive see dick die don't be a dick!
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CaptainKirk

Registered: 07/15/05
Posts: 2,478
Loc: gone
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Re: Prints in squat wide-mouth glass jars. [Re: kiss]
#5276404 - 02/08/06 07:09 AM (17 years, 11 months ago) |
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great post as usual thanks for the tip
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 26 days
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Re: Prints in squat wide-mouth glass jars. [Re: CaptainKirk]
#5276933 - 02/08/06 10:54 AM (17 years, 11 months ago) |
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Put another hole in the lid, and silicone on some tyvek or cover with micro pore tape, and youve got some air flow in there while the cap prints, to get darker prints.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Prints in squat wide-mouth glass jars. [Re: scatmanrav]
#5282180 - 02/09/06 03:05 PM (17 years, 11 months ago) |
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Yo, Scat, I added a variant method for better gas exchange & pictures in the lead off post.
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shroomsynergy
Fungi Freak


Registered: 01/17/06
Posts: 861
Loc: Andromeda
Last seen: 4 years, 4 months
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
#5282239 - 02/09/06 03:26 PM (17 years, 11 months ago) |
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nice tek man. were do YOU get your injector sites?
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Prints in squat wide-mouth glass jars. [Re: shroomsynergy]
#5282247 - 02/09/06 03:29 PM (17 years, 11 months ago) |
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I use ports off vacutainers.
I had a link to a site that sold just ports. But, it's dead now
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
#5282259 - 02/09/06 03:34 PM (17 years, 11 months ago) |
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I've been doing the Tyvek lid version minus the cap switch at the end. To extract spores, I just scrape them with the syringe tip, squirt some sterilized water onto them and suck them back up. This is done in a glove box, of course.
This version is almost exactly what I was planning on doing for future spore syringes, but I was going to use plastic lids with Tyvek/micropore covering one hole and RTV sealant for an injection port.
My ultrasonic cleaner will be here in a day or two and I've never used one before. My question is: Will the ultrasonic splash water onto the Tyvek/micropore? (contam concerns)
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Quick WBS Prep
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onetime
onetime


Registered: 11/13/03
Posts: 3,609
Last seen: 13 years, 1 month
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
#5282287 - 02/09/06 03:42 PM (17 years, 11 months ago) |
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http://vwrlabshop.com/product.asp_Q_pn_E_0012621
these tubes could also be used for great lc's
The ones with out the serium seperators. Just inject some lc solution and some spores and your good to go/ 20$ for a 100 of them.
--------------------
See? Yes, with my own three eyes. Depression, Misspells , wanting everying thing i cant have haveing nothing i want
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Prints in squat wide-mouth glass jars. [Re: FooMan]
#5282355 - 02/09/06 03:59 PM (17 years, 11 months ago) |
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My ultrasonic cleaner doesn't splash water around.
 Don't submerge the jar. Just fill the botton with a half inch of water. Set the jar in it & turn the puppy on. (ADVISE a TEST RUN ....with a blank jar..first). As YOURS may DIFFER
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shroomsynergy
Fungi Freak


Registered: 01/17/06
Posts: 861
Loc: Andromeda
Last seen: 4 years, 4 months
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
#5283112 - 02/09/06 07:29 PM (17 years, 11 months ago) |
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BTW, do you have a good constant and cheep scource for these jars that you could share??? that would be fabultastic!!  
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Prints in squat wide-mouth glass jars. [Re: shroomsynergy]
#5283289 - 02/09/06 08:17 PM (17 years, 11 months ago) |
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Most grocery, chain Marts & craft stores carry them.
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Edited by agar (02/09/06 09:05 PM)
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MrMaddHatter
Dementia praecox


Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
#5283395 - 02/09/06 08:42 PM (17 years, 11 months ago) |
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Quote:
I use ports off vacutainers. I had a link to a site that sold just ports. But, it's dead now
Is this the site ( 3rd product down} HERE Cause those are wonderful.
or, Were you getting those vacutainer plugs from somewhere singly?
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shroomsynergy
Fungi Freak


Registered: 01/17/06
Posts: 861
Loc: Andromeda
Last seen: 4 years, 4 months
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Re: Prints in squat wide-mouth glass jars. [Re: MrMaddHatter]
#5283413 - 02/09/06 08:46 PM (17 years, 11 months ago) |
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wow, thats the cheepest ive ever seen them. thanx a million for that !!!
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
#5283434 - 02/09/06 08:49 PM (17 years, 11 months ago) |
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<<<<click file save as>>>>
Excellent, now I have another reason to try out my new gun 
can you use a stirrer....I know that you also use ultrasonic cleaner.
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Atheist Chat
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Jaeger
Dreamer
Registered: 10/01/05
Posts: 960
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Re: Prints in squat wide-mouth glass jars. [Re: Snaggletooth]
#5283593 - 02/09/06 09:17 PM (17 years, 11 months ago) |
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I have used the blue plugs. They do work well. Nice and cheap too!
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Mycophreek423
Late For TheFuture!!


Registered: 01/07/06
Posts: 109
Loc: The Tenth Kingdom
Last seen: 4 years, 4 months
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Re: Prints in squat wide-mouth glass jars. [Re: Jaeger]
#5284074 - 02/09/06 11:11 PM (17 years, 11 months ago) |
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where could i find the blue plugs?
-------------------- I Thought You Where There , But you Where not. It Mushed Have Been Somthing I Ate.
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Jaeger
Dreamer
Registered: 10/01/05
Posts: 960
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Re: Prints in squat wide-mouth glass jars. [Re: MrMaddHatter]
#5284090 - 02/09/06 11:14 PM (17 years, 11 months ago) |
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Quote:
MrMaddHatter said:
Quote:
I use ports off vacutainers. I had a link to a site that sold just ports. But, it's dead now
Is this the site ( 3rd product down} HERE Cause those are wonderful.
or, Were you getting those vacutainer plugs from somewhere singly?
Here bud
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Re: Prints in squat wide-mouth glass jars. [Re: Mycophreek423]
#5284092 - 02/09/06 11:14 PM (17 years, 11 months ago) |
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In the link MrMaddHatter posted.
::EDIT:: gah.. slow again.
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Edited by tiny_rabid_birds (02/09/06 11:15 PM)
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
#5287215 - 02/10/06 08:31 PM (17 years, 11 months ago) |
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OK, I got the ultrasonic and tested this out. The spores aren't breaking up and dispersing very well. Is this because I didn't add Jet Dry to the water or because I didn't buy a really expensive ultrasonic? This is a small Sonic Wave ultrasonic with a "42,000 cycle".
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Quick WBS Prep
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 26 days
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Re: Prints in squat wide-mouth glass jars. [Re: MrMaddHatter]
#5287626 - 02/10/06 11:26 PM (17 years, 11 months ago) |
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Quote:
MrMaddHatter said:
Quote:
I use ports off vacutainers. I had a link to a site that sold just ports. But, it's dead now
Is this the site ( 3rd product down} HERE Cause those are wonderful.
or, Were you getting those vacutainer plugs from somewhere singly?
Thats where I get mine...
Yep, that works great for the air exchange too Mine were also meant for LC's (print on the bottom fo the jar, then inject LC solution, then just let it grow)
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dzagama
Stranger

Registered: 08/05/07
Posts: 20
Last seen: 14 years, 9 months
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
#7264914 - 08/06/07 01:26 AM (16 years, 5 months ago) |
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Thanks for this truly fantastic tek. Maybe this is a newb question, but how can one reduce the chance of introducing contaminants from the mushroom cap, itself? Dunking in peroxide?
- dzagama
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steluta
Stranger
Registered: 08/06/07
Posts: 1
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Re: Prints in squat wide-mouth glass jars. *DELETED* [Re: dzagama]
#7265441 - 08/06/07 08:23 AM (16 years, 5 months ago) |
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Post deleted by WronguyReason for deletion: Spamming
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Re: Prints in squat wide-mouth glass jars. [Re: steluta]
#7265472 - 08/06/07 08:32 AM (16 years, 5 months ago) |
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Oh god..
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: Prints in squat wide-mouth glass jars. [Re: steluta]
#7265508 - 08/06/07 08:50 AM (16 years, 5 months ago) |
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.
--------------------
AMU Q&A thread.
Edited by RogerRabbit (05/12/14 10:36 PM)
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deucedbi9
Stranger

Registered: 10/24/06
Posts: 4,594
Loc: UK
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Re: Prints in squat wide-mouth glass jars. [Re: The shroomy 1]
#7265573 - 08/06/07 09:21 AM (16 years, 5 months ago) |
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Quote:
dzagama said: Thanks for this truly fantastic tek. Maybe this is a newb question, but how can one reduce the chance of introducing contaminants from the mushroom cap, itself? Dunking in peroxide?
- dzagama
by not allowing the cap to touch the glass.
i do this(from a similar thread-> http://www.shroomery.org/forums/showflat.php/Number/6748859#Post6748859)->
you can fold a piece of tyvek in half,cut out a semi-circle and flatten back out. place the cap over the hole,and place on top of the jar. cover for 12-24 hours. cover with a new piece of sterilized tyvek (without hole)secure with a rubber band. the tyvek will allow the spore to dry untill ready to use.
if you want to keep the spore for a later date,secure some tin foil over the top with another rubber band.
-------------------- whether low pressure sucks or high pressure blows... it's a bugger to cycle in. even though I'm feeling good Something tells me I'd better activate my prayer capsule
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: Prints in squat wide-mouth glass jars. [Re: deucedbi9]
#7266060 - 08/06/07 11:47 AM (16 years, 5 months ago) |
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Ya know with smokes being nearly 8 bucks a pack in the county I live in I would have welcomed this spam...Except as far as I can see no-one sells Kools(The only cigarettes menthol enough for me since Newport quit making "Newport ice")
Guess its back to trying to figure out how to freebase vaporub for me
O and in the meantime >>>>
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deucedbi9
Stranger

Registered: 10/24/06
Posts: 4,594
Loc: UK
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Re: Prints in squat wide-mouth glass jars. [Re: Blutjager]
#7266207 - 08/06/07 12:26 PM (16 years, 5 months ago) |
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you want to ban me blut  i'd never try sell you an addictive drug!  shroomy 1 maybe you need to edit that quote,gotta protect the kids ya know
-------------------- whether low pressure sucks or high pressure blows... it's a bugger to cycle in. even though I'm feeling good Something tells me I'd better activate my prayer capsule
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: Prints in squat wide-mouth glass jars. [Re: deucedbi9]
#7266359 - 08/06/07 01:07 PM (16 years, 5 months ago) |
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Quote:
deucedbi9 said: you want to ban me blut  i'd never try sell you an addictive drug!  shroomy 1 maybe you need to edit that quote,gotta protect the kids ya know
No,No,not your,the guy who cave the cheap smokes links that dont sell Kools
Edited by Blutjager (08/06/07 01:08 PM)
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deucedbi9
Stranger

Registered: 10/24/06
Posts: 4,594
Loc: UK
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Re: Prints in squat wide-mouth glass jars. [Re: dzagama]
#7266536 - 08/06/07 02:00 PM (16 years, 5 months ago) |
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>No,No,not your,the guy who cave the cheap smokes links that dont sell Kools<
what  getting back on track... the reason for posting a reply to "dzagama's" reawakening of an old thread was to point out that... putting an undoubtedly contaminated cap into a sterilized jar is counterproductive. why bother sterilizing the jar, or the water your going to make the syringe with, if your going to put an obviously contaminated cap in the jar?
i'm not suggesting that my method will eliminate contaminants,they are still from an un-sterile fc after all,but that there will be fewer.
-------------------- whether low pressure sucks or high pressure blows... it's a bugger to cycle in. even though I'm feeling good Something tells me I'd better activate my prayer capsule
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dzagama
Stranger

Registered: 08/05/07
Posts: 20
Last seen: 14 years, 9 months
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Re: Prints in squat wide-mouth glass jars. [Re: deucedbi9]
#7268942 - 08/07/07 01:30 AM (16 years, 5 months ago) |
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Yes, I was mainly wondering if there was some kind of disinfecting procedure you could put the cap through before letting the spores drop.
Regardless, if people are having success with this tek, which I'm assuming the dormancy of the thread implies, then perhaps I'm just being an over-worrying newb. 
----
Ah, with the spam, I totally missed deucedbi9's informative reply. I'll give that a shot!
Thanks!
Edited by dzagama (08/07/07 01:20 PM)
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Testertips
all good


Registered: 03/09/14
Posts: 679
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Re: Prints in squat wide-mouth glass jars. [Re: dzagama]
#19978535 - 05/12/14 09:00 PM (9 years, 8 months ago) |
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So I'm having issues getting the dry spores to break off... what's the solution?
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Testertips
all good


Registered: 03/09/14
Posts: 679
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Re: Prints in squat wide-mouth glass jars. [Re: Testertips]
#19978537 - 05/12/14 09:01 PM (9 years, 8 months ago) |
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Should I have not extracted before they were fully dry and cemented to the glass?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Prints in squat wide-mouth glass jars. [Re: Testertips]
#19978590 - 05/12/14 09:12 PM (9 years, 8 months ago) |
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Well for starters ya don't bump an 8 year old thread
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Testertips
all good


Registered: 03/09/14
Posts: 679
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Re: Prints in squat wide-mouth glass jars. [Re: Pastywhyte]
#19978608 - 05/12/14 09:15 PM (9 years, 8 months ago) |
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holy... hahah didn't realize how old this thread was.
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Sl8t3
Skeptical


Registered: 06/22/13
Posts: 76
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Re: Prints in squat wide-mouth glass jars. [Re: Pastywhyte]
#19978610 - 05/12/14 09:16 PM (9 years, 8 months ago) |
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It was worth seeing the sig above (Jurassic park -- bum to window smashing)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Prints in squat wide-mouth glass jars. [Re: Blutjager]
#19978721 - 05/12/14 09:36 PM (9 years, 8 months ago) |
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Quote:
Blutjager said: Ya know with smokes being nearly 8 bucks a pack in the county I live in I would have welcomed this spam...
this guy has gottqa hate the prices today i just shoot the syringe at the print and what comes off comes off, for agar work a little water will help lift the print
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Testertips
all good


Registered: 03/09/14
Posts: 679
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Re: Prints in squat wide-mouth glass jars. [Re: cronicr]
#19978854 - 05/12/14 10:02 PM (9 years, 8 months ago) |
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Quote:
cronicr said:
Quote:
Blutjager said: Ya know with smokes being nearly 8 bucks a pack in the county I live in I would have welcomed this spam...
i just shoot the syringe at the print and what comes off comes off
not for me... shit is cemented on
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Prints in squat wide-mouth glass jars. [Re: Testertips]
#19978860 - 05/12/14 10:03 PM (9 years, 8 months ago) |
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Quote:
Testertips said:
Quote:
cronicr said:
Quote:
Blutjager said: Ya know with smokes being nearly 8 bucks a pack in the county I live in I would have welcomed this spam...
i just shoot the syringe at the print and what comes off comes off
not for me... shit is cemented on
just leave the water there for a bit theywill lift as they hydrate, you don't need the whole print to come up either
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Testertips
all good


Registered: 03/09/14
Posts: 679
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Re: Prints in squat wide-mouth glass jars. [Re: cronicr]
#19978864 - 05/12/14 10:04 PM (9 years, 8 months ago) |
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Quote:
cronicr said:
Quote:
Testertips said:
Quote:
cronicr said:
Quote:
Blutjager said: Ya know with smokes being nearly 8 bucks a pack in the county I live in I would have welcomed this spam...
i just shoot the syringe at the print and what comes off comes off
not for me... shit is cemented on
just leave the water there for a bit theywill lift as they hydrate, you don't need the whole print to come up either
ahh... i'll check that out.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Prints in squat wide-mouth glass jars. [Re: Testertips]
#19979029 - 05/12/14 10:39 PM (9 years, 8 months ago) |
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I think that's the first time I ever edited a six year old thread. 
Print on wax paper and then wrap it up in foil if you want a method that's easy to get the spores off. Be careful though because if you tilt a wax paper print on its side, all the spores will slide right off. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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spore baby



Registered: 07/30/13
Posts: 4,918
Last seen: 8 years, 7 months
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
#19979721 - 05/13/14 01:57 AM (9 years, 8 months ago) |
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Nice little jars.
Oops, Roger Rabbit, I didn't pay attention how old this thread was.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Prints in squat wide-mouth glass jars. [Re: RogerRabbit]
#19979724 - 05/13/14 01:59 AM (9 years, 8 months ago) |
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Quote:
RogerRabbit said: I think that's the first time I ever edited a six year old thread. 
RR
made me look just to see you just wiped the whole post out that bad huh
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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thelanzii

Registered: 11/13/12
Posts: 5,434
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Re: Prints in squat wide-mouth glass jars. [Re: Jaeger]
#25039013 - 03/04/18 07:21 PM (5 years, 10 months ago) |
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dope tek
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