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Invisibleagar
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Prints in squat wide-mouth glass jars. * 6
    #5276177 - 02/08/06 02:36 AM (17 years, 11 months ago)

Nothing NEW here.
Just advise for new hands, who don't know.

Ball has a 250 ml SQUAT wide mouth canning jar out.










Simply drill a hole in the lid & install an injection port.

If you don't have an injection port.
Drill an 1/8th inch hole in the lid.
Scuff the surface, around the hole.
(both sides, so sealant sticks better)

Dab on some RTV silicone high temp gasket sealer over the hole. (both sides).
Allow to cure at least overnight.

PC jar 20 minutes @ 15 psi, with lid LOOSE.
(sterilizes it)
Remove from PC hot & tighten lid.

In a glove box, or under a flow hood.
Cleanly severe a a mushroom cap, place in print jar.
Leave lid on 3/4 turn LOOSE
(gas exchange - so you don't get condensation from a wet cap).

Allow print to set 24 (+/-)hours.
In glove box, carefully remove cap & replace lid.
Leave lid 3/4 turn LOOSE for another 24 hours
(place in mild indirect sunlight, so the print dries).

Tighten lid TIGHT.

Knock/rap jar on semi/hard surface to dislodge spores.

With a large capacity syringe.
(or whatever syringe size you have)

Inject 125 / 200 ml of STERILE water.
Shake jar violently.

Hint - put a few drops of Jet Dry in the STERILE water.

Hint - to make STERILE WATER.
simply PC another jar (as above).
With STERILE water & Jet Dry solution in it.
(Jet Dry serves as an anti-clumping agent)
(hint - distilled water works best)

There you have it.
Enough spore solution to fill a dozen + 10 ml syringes.

For those with an ultrasonic cleaner.

Before making new spore syringes.
Place sealed jar in it, for an hour.
(disperses spores PERFECTLY, so all syringes are fairly equal)

Hint, once print jar is almost EMPTY.
Inject 150 ml of sterilized nutrient solution, to make LC. :grin:
~~~~~~~~~~~~~~~~
EDIT TO ADD A VARIANT GAS EXCHANGE METHOD



For better gas exchange.
Cut a small piece of tyvek to cover the lid.
Place tyvek over the jar mouth.
Screw lid on TIGHT, to form the tyvek to lid shape.
Use a razor, or Exacto knife to trim off excess tyvek.

PC the jar, with the tyvek lid in place.
In glove box, cleanly sever mushroom cap.
Remove jar ring & tyvek.
Place mushroom cap in jar.
Replace tyvek & jar ring.
Screw ring on TIGHT.

Allow 24-36 hours for print to set thickly.
Remove cap, replace tyvek & jar ring, screw ring on tightly.
Allow 24 hours for print to DRY.

Remove tyvek, replace with sanitized injection port lid.


The object of this variant is:
1 > to allow for optimal gas exchange.
2 > So, the cap doesn't begin to rot.
3 > so the print dries quickly.

EDIT (again)

Do not try to pound big caps into little jars.
:grin: :rolleyes:


Edited by agar (02/13/06 07:18 AM)


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Invisibletiny_rabid_birds
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5276191 - 02/08/06 02:53 AM (17 years, 11 months ago)

Another wonderful little write-up.  That's a great idea to turn it into an LC jar once it's nearly cashed too.  Good job :thumbup:

Where do you get the injection ports from?


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Offlineblackout
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Re: Prints in squat wide-mouth glass jars. [Re: tiny_rabid_birds]
    #5276198 - 02/08/06 02:58 AM (17 years, 11 months ago)

Good write up. I use those shallow glass salsa dip jars like that.


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Offlinekiss
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Re: Prints in squat wide-mouth glass jars. [Re: blackout]
    #5276326 - 02/08/06 05:16 AM (17 years, 11 months ago)

Thats the jars I was talking about Agar. Mine are abit smaller though. Almost the size of baby food jars.


--------------------
see dick drink
see dick drive
see dick die
don't be a dick!


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InvisibleCaptainKirk

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Re: Prints in squat wide-mouth glass jars. [Re: kiss]
    #5276404 - 02/08/06 07:09 AM (17 years, 11 months ago)

great post as usual :wink: :thumbup: thanks for the tip :stoned:


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Offlinescatmanrav
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Re: Prints in squat wide-mouth glass jars. [Re: CaptainKirk]
    #5276933 - 02/08/06 10:54 AM (17 years, 11 months ago)



Put another hole in the lid, and silicone on some tyvek or cover with micro pore tape, and youve got some air flow in there while the cap prints, to get darker prints.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Invisibleagar
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Re: Prints in squat wide-mouth glass jars. [Re: scatmanrav]
    #5282180 - 02/09/06 03:05 PM (17 years, 11 months ago)

Yo, Scat,
I added a variant method for better gas exchange & pictures in the lead off post. :wink:


--------------------


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Offlineshroomsynergy
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5282239 - 02/09/06 03:26 PM (17 years, 11 months ago)

nice tek man. were do YOU get your injector sites?
:getstoned: :stinkyshroom: :guinness: :stinkyshroom: :getstoned:


--------------------


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Invisibleagar
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Re: Prints in squat wide-mouth glass jars. [Re: shroomsynergy]
    #5282247 - 02/09/06 03:29 PM (17 years, 11 months ago)

I use ports off vacutainers.

I had a link to a site that sold just ports.
But, it's dead now :rolleyes:


--------------------


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InvisibleFooMan
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5282259 - 02/09/06 03:34 PM (17 years, 11 months ago)

I've been doing the Tyvek lid version minus the cap switch at the end. To extract spores, I just scrape them with the syringe tip, squirt some sterilized water onto them and suck them back up. This is done in a glove box, of course.

This version is almost exactly what I was planning on doing for future spore syringes, but I was going to use plastic lids with Tyvek/micropore covering one hole and RTV sealant for an injection port.

My ultrasonic cleaner will be here in a day or two and I've never used one before. My question is: Will the ultrasonic splash water onto the Tyvek/micropore? (contam concerns)


--------------------

Quick WBS Prep


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Offlineonetime
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5282287 - 02/09/06 03:42 PM (17 years, 11 months ago)

http://vwrlabshop.com/product.asp_Q_pn_E_0012621

these tubes could also be used for great lc's





The ones with out the serium seperators. Just inject some lc solution and some spores and your good to go/ 20$ for a 100 of them.


--------------------

See?
Yes, with my own three eyes.
Depression, Misspells , wanting everying thing i cant have haveing nothing i want


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Invisibleagar
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Re: Prints in squat wide-mouth glass jars. [Re: FooMan]
    #5282355 - 02/09/06 03:59 PM (17 years, 11 months ago)

My ultrasonic cleaner doesn't splash water around.

Don't submerge the jar.
Just fill the botton with a half inch of water.
Set the jar in it & turn the puppy on.
(ADVISE a TEST RUN ....with a blank jar..first).
As YOURS may DIFFER


--------------------


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Offlineshroomsynergy
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5283112 - 02/09/06 07:29 PM (17 years, 11 months ago)

BTW, do you have a good constant and cheep scource for these jars that you could share??? that would be fabultastic!!
:bowdown: :bowdown: :bowdown:
      :bowdown: :bowdown: :bowdown:
              :bowdown: :bowdown: :bowdown:


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Invisibleagar
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Re: Prints in squat wide-mouth glass jars. [Re: shroomsynergy]
    #5283289 - 02/09/06 08:17 PM (17 years, 11 months ago)

Most grocery, chain Marts & craft stores carry them.


--------------------


Edited by agar (02/09/06 09:05 PM)


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InvisibleMrMaddHatter
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5283395 - 02/09/06 08:42 PM (17 years, 11 months ago)

Quote:

I use ports off vacutainers.
I had a link to a site that sold just ports.
But, it's dead now




Is this the site ( 3rd product down} HERE
Cause those are wonderful.

or, Were you getting those vacutainer plugs from somewhere singly?


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Offlineshroomsynergy
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Re: Prints in squat wide-mouth glass jars. [Re: MrMaddHatter]
    #5283413 - 02/09/06 08:46 PM (17 years, 11 months ago)

wow, thats the cheepest ive ever seen them. thanx a million for that !!! :thumbup: :thumbup: :thumbup: :thumbup: :thumbup:


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OfflineSnaggletooth
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5283434 - 02/09/06 08:49 PM (17 years, 11 months ago)

<<<<click file save as>>>>


Excellent, now I have another reason to try out my new gun

can you use a stirrer....I know that you also use ultrasonic cleaner.


--------------------


Atheist Chat


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InvisibleJaeger
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Re: Prints in squat wide-mouth glass jars. [Re: Snaggletooth]
    #5283593 - 02/09/06 09:17 PM (17 years, 11 months ago)

I have used the blue plugs. They do work well. Nice and cheap too!


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OfflineMycophreek423
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Re: Prints in squat wide-mouth glass jars. [Re: Jaeger]
    #5284074 - 02/09/06 11:11 PM (17 years, 11 months ago)

where could i find the blue plugs?


--------------------
I Thought You Where There , But you Where not. It Mushed Have Been Somthing I Ate.


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InvisibleJaeger
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Re: Prints in squat wide-mouth glass jars. [Re: MrMaddHatter]
    #5284090 - 02/09/06 11:14 PM (17 years, 11 months ago)

Quote:

MrMaddHatter said:
Quote:

I use ports off vacutainers.
I had a link to a site that sold just ports.
But, it's dead now




Is this the site ( 3rd product down} HERE
Cause those are wonderful.

or, Were you getting those vacutainer plugs from somewhere singly?




Here bud :smile:


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Invisibletiny_rabid_birds
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Re: Prints in squat wide-mouth glass jars. [Re: Mycophreek423]
    #5284092 - 02/09/06 11:14 PM (17 years, 11 months ago)

In the link MrMaddHatter posted.

::EDIT:: gah.. slow again.


--------------------


Edited by tiny_rabid_birds (02/09/06 11:15 PM)


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InvisibleFooMan
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5287215 - 02/10/06 08:31 PM (17 years, 11 months ago)

OK, I got the ultrasonic and tested this out. The spores aren't breaking up and dispersing very well. Is this because I didn't add Jet Dry to the water or because I didn't buy a really expensive ultrasonic? This is a small Sonic Wave ultrasonic with a "42,000 cycle".


--------------------

Quick WBS Prep


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Offlinescatmanrav
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Re: Prints in squat wide-mouth glass jars. [Re: MrMaddHatter]
    #5287626 - 02/10/06 11:26 PM (17 years, 11 months ago)

Quote:

MrMaddHatter said:
Quote:

I use ports off vacutainers.
I had a link to a site that sold just ports.
But, it's dead now




Is this the site ( 3rd product down} HERE
Cause those are wonderful.

or, Were you getting those vacutainer plugs from somewhere singly?




Thats where I get mine...

Yep, that works great for the air exchange too :smile: Mine were also meant for LC's (print on the bottom fo the jar, then inject LC solution, then just let it grow)


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinedzagama
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #7264914 - 08/06/07 01:26 AM (16 years, 5 months ago)

Thanks for this truly fantastic tek. Maybe this is a newb question, but how can one reduce the chance of introducing contaminants from the mushroom cap, itself? Dunking in peroxide?

- dzagama


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Offlinesteluta
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Re: Prints in squat wide-mouth glass jars. *DELETED* [Re: dzagama]
    #7265441 - 08/06/07 08:23 AM (16 years, 5 months ago)

Post deleted by Wronguy

Reason for deletion: Spamming



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OfflineNeobean
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Re: Prints in squat wide-mouth glass jars. [Re: steluta]
    #7265472 - 08/06/07 08:32 AM (16 years, 5 months ago)

Oh god..


--------------------
If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!


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OfflineThe shroomy 1
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Re: Prints in squat wide-mouth glass jars. [Re: steluta]
    #7265508 - 08/06/07 08:50 AM (16 years, 5 months ago)


.


--------------------


AMU Q&A thread.


Edited by RogerRabbit (05/12/14 10:36 PM)


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Invisibledeucedbi9
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Re: Prints in squat wide-mouth glass jars. [Re: The shroomy 1]
    #7265573 - 08/06/07 09:21 AM (16 years, 5 months ago)

Quote:

dzagama said:
Thanks for this truly fantastic tek. Maybe this is a newb question, but how can one reduce the chance of introducing contaminants from the mushroom cap, itself? Dunking in peroxide?

- dzagama



by not allowing the cap to touch the glass.

i do this(from a similar thread->
http://www.shroomery.org/forums/showflat.php/Number/6748859#Post6748859)->


you can fold a piece of tyvek in half,cut out a semi-circle and flatten back out.
place the cap over the hole,and place on top of the jar.
cover for 12-24 hours.
cover with a new piece of sterilized tyvek (without hole)secure with a rubber band.
the tyvek will allow the spore to dry untill ready to use.

if you want to keep the spore for a later date,secure some tin foil
over the top with another rubber band.


--------------------
whether low pressure sucks or high pressure blows...
it's a bugger to cycle in.

even though I'm feeling good
Something tells me I'd better activate my prayer capsule


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InvisibleBlutjager
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Re: Prints in squat wide-mouth glass jars. [Re: deucedbi9]
    #7266060 - 08/06/07 11:47 AM (16 years, 5 months ago)

Ya know with smokes being nearly 8 bucks a pack in the county I live in I would have welcomed this spam...Except as far as I can see no-one sells Kools(The only cigarettes menthol enough for me since Newport quit making "Newport ice")

Guess its back to trying to figure out how to freebase vaporub for me:hehehe:

O and in the meantime >>>>  :banbanban:  :rules:  :banned:  :ban:


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Invisibledeucedbi9
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Re: Prints in squat wide-mouth glass jars. [Re: Blutjager]
    #7266207 - 08/06/07 12:26 PM (16 years, 5 months ago)

you want to ban me blut :sad:
i'd never try sell you an addictive drug! :wink:
shroomy 1
maybe you need to edit that quote,gotta protect the kids ya know :grin:


--------------------
whether low pressure sucks or high pressure blows...
it's a bugger to cycle in.

even though I'm feeling good
Something tells me I'd better activate my prayer capsule


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InvisibleBlutjager
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Re: Prints in squat wide-mouth glass jars. [Re: deucedbi9]
    #7266359 - 08/06/07 01:07 PM (16 years, 5 months ago)

Quote:

deucedbi9 said:
you want to ban me blut :sad:
i'd never try sell you an addictive drug! :wink:
shroomy 1
maybe you need to edit that quote,gotta protect the kids ya know :grin:




No,No,not your,the guy who cave the cheap smokes links that dont sell Kools:yesnod:


Edited by Blutjager (08/06/07 01:08 PM)


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Invisibledeucedbi9
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Re: Prints in squat wide-mouth glass jars. [Re: dzagama]
    #7266536 - 08/06/07 02:00 PM (16 years, 5 months ago)

>No,No,not your,the guy who cave the cheap smokes links that dont sell Kools<

what :confused:
getting back on track...
the reason for posting a reply to "dzagama's" reawakening of an old thread was to point out that...
putting an undoubtedly contaminated cap into a sterilized jar is counterproductive.
why bother sterilizing the jar, or the water your going to make the syringe with, if your going to put an obviously contaminated cap in the jar?

i'm not suggesting that my method will eliminate contaminants,they are still from an un-sterile fc after all,but that there will be fewer.


--------------------
whether low pressure sucks or high pressure blows...
it's a bugger to cycle in.

even though I'm feeling good
Something tells me I'd better activate my prayer capsule


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Offlinedzagama
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Re: Prints in squat wide-mouth glass jars. [Re: deucedbi9]
    #7268942 - 08/07/07 01:30 AM (16 years, 5 months ago)

Yes, I was mainly wondering if there was some kind of disinfecting procedure you could put the cap through before letting the spores drop.

Regardless, if people are having success with this tek, which I'm assuming the dormancy of the thread implies, then perhaps I'm just being an over-worrying newb. :wink:

----

Ah, with the spam, I totally missed deucedbi9's informative reply.  I'll give that a shot!


Thanks!


Edited by dzagama (08/07/07 01:20 PM)


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InvisibleTestertips
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Re: Prints in squat wide-mouth glass jars. [Re: dzagama]
    #19978535 - 05/12/14 09:00 PM (9 years, 8 months ago)

So I'm having issues getting the dry spores to break off... what's the solution?


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InvisibleTestertips
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Re: Prints in squat wide-mouth glass jars. [Re: Testertips]
    #19978537 - 05/12/14 09:01 PM (9 years, 8 months ago)

Should I have not extracted before they were fully dry and cemented to the glass?


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InvisiblePastywhyteMDiscord
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Re: Prints in squat wide-mouth glass jars. [Re: Testertips]
    #19978590 - 05/12/14 09:12 PM (9 years, 8 months ago)

Well for starters ya don't bump an 8 year old thread :lmafo:


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InvisibleTestertips
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Re: Prints in squat wide-mouth glass jars. [Re: Pastywhyte]
    #19978608 - 05/12/14 09:15 PM (9 years, 8 months ago)

holy... hahah didn't realize how old this thread was.


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InvisibleSl8t3
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Re: Prints in squat wide-mouth glass jars. [Re: Pastywhyte]
    #19978610 - 05/12/14 09:16 PM (9 years, 8 months ago)

It was worth seeing the sig above (Jurassic park -- bum to window smashing)


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OfflinecronicrFacebook
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Re: Prints in squat wide-mouth glass jars. [Re: Blutjager]
    #19978721 - 05/12/14 09:36 PM (9 years, 8 months ago)

Quote:

Blutjager said:
Ya know with smokes being nearly 8 bucks a pack in the county I live in I would have welcomed this spam...



this guy has gottqa hate the prices today:crazy: i just shoot the syringe at the print and what comes off comes off, for agar work a little water will help lift the print


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleTestertips
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Re: Prints in squat wide-mouth glass jars. [Re: cronicr]
    #19978854 - 05/12/14 10:02 PM (9 years, 8 months ago)

Quote:

cronicr said:
Quote:

Blutjager said:
Ya know with smokes being nearly 8 bucks a pack in the county I live in I would have welcomed this spam...



i just shoot the syringe at the print and what comes off comes off




not for me... shit is cemented on


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OfflinecronicrFacebook
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Re: Prints in squat wide-mouth glass jars. [Re: Testertips]
    #19978860 - 05/12/14 10:03 PM (9 years, 8 months ago)

Quote:

Testertips said:
Quote:

cronicr said:
Quote:

Blutjager said:
Ya know with smokes being nearly 8 bucks a pack in the county I live in I would have welcomed this spam...



i just shoot the syringe at the print and what comes off comes off




not for me... shit is cemented on



just leave the water there for a bit theywill lift as they hydrate, you don't need the whole print to come up either


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleTestertips
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Posts: 679
Re: Prints in squat wide-mouth glass jars. [Re: cronicr]
    #19978864 - 05/12/14 10:04 PM (9 years, 8 months ago)

Quote:

cronicr said:
Quote:

Testertips said:
Quote:

cronicr said:
Quote:

Blutjager said:
Ya know with smokes being nearly 8 bucks a pack in the county I live in I would have welcomed this spam...



i just shoot the syringe at the print and what comes off comes off




not for me... shit is cemented on



just leave the water there for a bit theywill lift as they hydrate, you don't need the whole print to come up either




ahh... i'll check that out.


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OfflineRogerRabbitM
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Re: Prints in squat wide-mouth glass jars. [Re: Testertips]
    #19979029 - 05/12/14 10:39 PM (9 years, 8 months ago)

I think that's the first time I ever edited a six year old thread. :tongue:

Print on wax paper and then wrap it up in foil if you want a method that's easy to get the spores off.  Be careful though because if you tilt a wax paper print on its side, all the spores will slide right off.
RR


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Offlinespore baby
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #19979721 - 05/13/14 01:57 AM (9 years, 8 months ago)

Nice little jars.

Oops, :thanx: Roger Rabbit, I didn't pay attention how old this thread was.

:aweohyou:


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OfflinecronicrFacebook
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Re: Prints in squat wide-mouth glass jars. [Re: RogerRabbit]
    #19979724 - 05/13/14 01:59 AM (9 years, 8 months ago)

Quote:

RogerRabbit said:
I think that's the first time I ever edited a six year old thread. :tongue:

RR



made me look just to see you just wiped the whole post out:lol: that bad huh


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I'm tired do me a favor


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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,434
Re: Prints in squat wide-mouth glass jars. [Re: Jaeger]
    #25039013 - 03/04/18 07:21 PM (5 years, 10 months ago)

dope tek


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