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Invisibleagar
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Prints in squat wide-mouth glass jars. * 4
    #5276177 - 02/08/06 03:36 AM (15 years, 23 days ago)

Nothing NEW here.
Just advise for new hands, who don't know.

Ball has a 250 ml SQUAT wide mouth canning jar out.










Simply drill a hole in the lid & install an injection port.

If you don't have an injection port.
Drill an 1/8th inch hole in the lid.
Scuff the surface, around the hole.
(both sides, so sealant sticks better)

Dab on some RTV silicone high temp gasket sealer over the hole. (both sides).
Allow to cure at least overnight.

PC jar 20 minutes @ 15 psi, with lid LOOSE.
(sterilizes it)
Remove from PC hot & tighten lid.

In a glove box, or under a flow hood.
Cleanly severe a a mushroom cap, place in print jar.
Leave lid on 3/4 turn LOOSE
(gas exchange - so you don't get condensation from a wet cap).

Allow print to set 24 (+/-)hours.
In glove box, carefully remove cap & replace lid.
Leave lid 3/4 turn LOOSE for another 24 hours
(place in mild indirect sunlight, so the print dries).

Tighten lid TIGHT.

Knock/rap jar on semi/hard surface to dislodge spores.

With a large capacity syringe.
(or whatever syringe size you have)

Inject 125 / 200 ml of STERILE water.
Shake jar violently.

Hint - put a few drops of Jet Dry in the STERILE water.

Hint - to make STERILE WATER.
simply PC another jar (as above).
With STERILE water & Jet Dry solution in it.
(Jet Dry serves as an anti-clumping agent)
(hint - distilled water works best)

There you have it.
Enough spore solution to fill a dozen + 10 ml syringes.

For those with an ultrasonic cleaner.

Before making new spore syringes.
Place sealed jar in it, for an hour.
(disperses spores PERFECTLY, so all syringes are fairly equal)

Hint, once print jar is almost EMPTY.
Inject 150 ml of sterilized nutrient solution, to make LC. :grin:
~~~~~~~~~~~~~~~~
EDIT TO ADD A VARIANT GAS EXCHANGE METHOD



For better gas exchange.
Cut a small piece of tyvek to cover the lid.
Place tyvek over the jar mouth.
Screw lid on TIGHT, to form the tyvek to lid shape.
Use a razor, or Exacto knife to trim off excess tyvek.

PC the jar, with the tyvek lid in place.
In glove box, cleanly sever mushroom cap.
Remove jar ring & tyvek.
Place mushroom cap in jar.
Replace tyvek & jar ring.
Screw ring on TIGHT.

Allow 24-36 hours for print to set thickly.
Remove cap, replace tyvek & jar ring, screw ring on tightly.
Allow 24 hours for print to DRY.

Remove tyvek, replace with sanitized injection port lid.


The object of this variant is:
1 > to allow for optimal gas exchange.
2 > So, the cap doesn't begin to rot.
3 > so the print dries quickly.

EDIT (again)

Do not try to pound big caps into little jars.
:grin: :rolleyes:


Edited by agar (02/13/06 08:18 AM)


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Invisibletiny_rabid_birds
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5276191 - 02/08/06 03:53 AM (15 years, 23 days ago)

Another wonderful little write-up.  That's a great idea to turn it into an LC jar once it's nearly cashed too.  Good job :thumbup:

Where do you get the injection ports from?


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Offlineblackout
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Re: Prints in squat wide-mouth glass jars. [Re: tiny_rabid_birds]
    #5276198 - 02/08/06 03:58 AM (15 years, 23 days ago)

Good write up. I use those shallow glass salsa dip jars like that.


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Offlinekiss
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Re: Prints in squat wide-mouth glass jars. [Re: blackout]
    #5276326 - 02/08/06 06:16 AM (15 years, 23 days ago)

Thats the jars I was talking about Agar. Mine are abit smaller though. Almost the size of baby food jars.


--------------------
see dick drink
see dick drive
see dick die
don't be a dick!


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InvisibleCaptainKirk

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Re: Prints in squat wide-mouth glass jars. [Re: kiss]
    #5276404 - 02/08/06 08:09 AM (15 years, 23 days ago)

great post as usual :wink: :thumbup: thanks for the tip :stoned:


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Offlinescatmanrav
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Re: Prints in squat wide-mouth glass jars. [Re: CaptainKirk]
    #5276933 - 02/08/06 11:54 AM (15 years, 23 days ago)



Put another hole in the lid, and silicone on some tyvek or cover with micro pore tape, and youve got some air flow in there while the cap prints, to get darker prints.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Invisibleagar
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Re: Prints in squat wide-mouth glass jars. [Re: scatmanrav]
    #5282180 - 02/09/06 04:05 PM (15 years, 22 days ago)

Yo, Scat,
I added a variant method for better gas exchange & pictures in the lead off post. :wink:


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Offlineshroomsynergy
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5282239 - 02/09/06 04:26 PM (15 years, 22 days ago)

nice tek man. were do YOU get your injector sites?
:getstoned: :stinkyshroom: :guinness: :stinkyshroom: :getstoned:


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Invisibleagar
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Re: Prints in squat wide-mouth glass jars. [Re: shroomsynergy]
    #5282247 - 02/09/06 04:29 PM (15 years, 22 days ago)

I use ports off vacutainers.

I had a link to a site that sold just ports.
But, it's dead now :rolleyes:


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InvisibleFooManM
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5282259 - 02/09/06 04:34 PM (15 years, 22 days ago)

I've been doing the Tyvek lid version minus the cap switch at the end. To extract spores, I just scrape them with the syringe tip, squirt some sterilized water onto them and suck them back up. This is done in a glove box, of course.

This version is almost exactly what I was planning on doing for future spore syringes, but I was going to use plastic lids with Tyvek/micropore covering one hole and RTV sealant for an injection port.

My ultrasonic cleaner will be here in a day or two and I've never used one before. My question is: Will the ultrasonic splash water onto the Tyvek/micropore? (contam concerns)


--------------------

Quick WBS Prep


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Offlineonetime
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5282287 - 02/09/06 04:42 PM (15 years, 22 days ago)

http://vwrlabshop.com/product.asp_Q_pn_E_0012621

these tubes could also be used for great lc's





The ones with out the serium seperators. Just inject some lc solution and some spores and your good to go/ 20$ for a 100 of them.


--------------------

See?
Yes, with my own three eyes.
Depression, Misspells , wanting everying thing i cant have haveing nothing i want


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Invisibleagar
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Re: Prints in squat wide-mouth glass jars. [Re: FooMan]
    #5282355 - 02/09/06 04:59 PM (15 years, 22 days ago)

My ultrasonic cleaner doesn't splash water around.

Don't submerge the jar.
Just fill the botton with a half inch of water.
Set the jar in it & turn the puppy on.
(ADVISE a TEST RUN ....with a blank jar..first).
As YOURS may DIFFER


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Offlineshroomsynergy
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5283112 - 02/09/06 08:29 PM (15 years, 22 days ago)

BTW, do you have a good constant and cheep scource for these jars that you could share??? that would be fabultastic!!
:bowdown: :bowdown: :bowdown:
      :bowdown: :bowdown: :bowdown:
              :bowdown: :bowdown: :bowdown:


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Invisibleagar
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Re: Prints in squat wide-mouth glass jars. [Re: shroomsynergy]
    #5283289 - 02/09/06 09:17 PM (15 years, 22 days ago)

Most grocery, chain Marts & craft stores carry them.


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Edited by agar (02/09/06 10:05 PM)


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InvisibleMrMaddHatter
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5283395 - 02/09/06 09:42 PM (15 years, 22 days ago)

Quote:

I use ports off vacutainers.
I had a link to a site that sold just ports.
But, it's dead now




Is this the site ( 3rd product down} HERE
Cause those are wonderful.

or, Were you getting those vacutainer plugs from somewhere singly?


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Offlineshroomsynergy
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Re: Prints in squat wide-mouth glass jars. [Re: MrMaddHatter]
    #5283413 - 02/09/06 09:46 PM (15 years, 22 days ago)

wow, thats the cheepest ive ever seen them. thanx a million for that !!! :thumbup: :thumbup: :thumbup: :thumbup: :thumbup:


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OfflineSnaggletooth
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Re: Prints in squat wide-mouth glass jars. [Re: agar]
    #5283434 - 02/09/06 09:49 PM (15 years, 22 days ago)

<<<<click file save as>>>>


Excellent, now I have another reason to try out my new gun

can you use a stirrer....I know that you also use ultrasonic cleaner.


--------------------


Atheist Chat


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InvisibleJaeger
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Registered: 10/01/05
Posts: 960
Re: Prints in squat wide-mouth glass jars. [Re: Snaggletooth]
    #5283593 - 02/09/06 10:17 PM (15 years, 22 days ago)

I have used the blue plugs. They do work well. Nice and cheap too!


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OfflineMycophreek423
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Re: Prints in squat wide-mouth glass jars. [Re: Jaeger]
    #5284074 - 02/10/06 12:11 AM (15 years, 22 days ago)

where could i find the blue plugs?


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I Thought You Where There , But you Where not. It Mushed Have Been Somthing I Ate.


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InvisibleJaeger
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Re: Prints in squat wide-mouth glass jars. [Re: MrMaddHatter]
    #5284090 - 02/10/06 12:14 AM (15 years, 22 days ago)

Quote:

MrMaddHatter said:
Quote:

I use ports off vacutainers.
I had a link to a site that sold just ports.
But, it's dead now




Is this the site ( 3rd product down} HERE
Cause those are wonderful.

or, Were you getting those vacutainer plugs from somewhere singly?




Here bud :smile:


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