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OfflineSyd_barret
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Registered: 10/27/01
Posts: 306
Last seen: 12 years, 11 months
Air exchange for grain using quart jars..
    #527531 - 01/21/02 05:28 PM (21 years, 10 months ago)

Ok, tomorrow I plan on sterilizing a quart jar of rye grain, my question is do I have to use a filter disc or polyfill or something like that? Or can I just tape it and leave?
I've heard that you can but colonization is extremely slow...

If it is required (or highly recommended) where can I get some polyfill? (only tell me stores in real life, no online stuff please and don't guess only places you know that have it, I don't want to try a hundred different stores tomorrow)


Thank you.


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OfflineSyd_barret
The Greatest
Registered: 10/27/01
Posts: 306
Last seen: 12 years, 11 months
Re: Air exchange for grain using quart jars.. [Re: Syd_barret]
    #527590 - 01/21/02 06:37 PM (21 years, 10 months ago)

rabdargab bump


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Invisibleluvdemshrooms
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Registered: 11/29/01
Posts: 34,247
Loc: Lost In Space
Re: Air exchange for grain using quart jars.. [Re: Syd_barret]
    #527607 - 01/21/02 07:05 PM (21 years, 10 months ago)

I've been using quart jars. The first two batches I just used foil for the lid. I then put four jars each into a clear plastic garbage bag which I kept in a closet. (12 jars total) Lost two to contams, so did a friend who shared his print with me. Temps around 72f. After a week I loosened the foil enough to let air into the jars without opening the bag. I got what I feel were excellent results. This last time I bought the filters you see advertised on this site. (Micron Magic) I had a couple of jars lost to contams and a couple got waterlogged. Instead of a closet I made an incubator. The contams may have been because I lent my print to a friend who cut it in half with scissors. (Same friend as before) I believe he knows enough to sterilize the scissors but who knows. The water logged jars were from an idea I had. I laid them on there side thinking that I wouldn't have to put turn them upside down. Seems as if this was a mistake because the jars closet to the bubbler got much water in them. Yuk, what a mess. Having said all that, the filtered jars colonized in less than two weeks where as the bagged jars took almost a full month. Of course there is the temp difference to factor in. All said, I like the filters very much as they have sped up the process quite a bit. I do not feel the contams were from the filters since I did four strains and only one strain fell to contams.


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You cannot legislate the poor into prosperity by legislating the wealthy out of prosperity. What one person receives without working for another person must work for without receiving. The government cannot give to anybody anything that the government does not first take from somebody else. When half of the people get the idea that they do not have to work because the other half is going to take care of them and when the other half gets the idea that it does no good to work because somebody else is going to get what they work for that my dear friend is the beginning of the end of any nation. You cannot multiply wealth by dividing it. ~ Adrian Rogers


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InvisibleNighted
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Registered: 11/09/01
Posts: 698
Loc: Funkytown
Re: Air exchange for grain using quart jars.. [Re: Syd_barret]
    #527619 - 01/21/02 07:20 PM (21 years, 10 months ago)

If using canning jars and liquid inoculation using a syringe, drill a hole in the center of the lid using a 1/16" drill bit. Put the lid on the jar upside down. Now put a square of tin-foil over the lid and form the overlapping edge over the mouth of the jar so it doesn't rip when you put the band back on. Now put the screw band on over the tin-foil and the lid. Put a piece of tape over the hole you made.

When the jar is cooled and you're ready to inoculate, pull back the tape and poke your syringe through the hole. Put the tape back. Done.

After the first time you shake the jar to re-distribute the mycelium (~3 days) you can loosen the lid. I've seen jars colonize in under 10 days this way time and time again (even from multi-spore inoculations, the key is to shake the jar and loosen the lid at the proper time).


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OfflineSyd_barret
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Registered: 10/27/01
Posts: 306
Last seen: 12 years, 11 months
Re: Air exchange for grain using quart jars.. [Re: Nighted]
    #527675 - 01/21/02 08:49 PM (21 years, 10 months ago)

Can anyone give me a tek that explains all this?

I might be getting some polyfill so a tek using something like that would be nice..
Or just a tek that doesnt use filters at all...

thanks again.


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Anonymous

Re: Air exchange for grain using quart jars.. [Re: Syd_barret]
    #527956 - 01/22/02 04:52 AM (21 years, 10 months ago)



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