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mogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
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Re: Liquid culture parameters [Re: mogur]
#5347224 - 02/27/06 09:18 PM (17 years, 10 months ago) |
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The final result-
Below is a photo of all surviving liquid cultures.

I have learned that nutritional additives need to be watered down to about 20% of the total liquid volume. The potato/dextrose teks I read before this experiment led me astray. Most of them simply said to boil potatoes in a liter of water, filter, and add water back to 1 liter. This is wrong. More detailed broth teks and my own experience dictate a much more dilute solution. Also, short of vacuum filtering through extremely fine meshes, a settling period is required, and the clear supernatant extracted. Otherwise, the solution is so turbid (cloudy) that bacterial contams are almost impossible to identify.
Mycelium growth was observed in almost all cultures tested, but were so obscured by turbidity that hardly any useful data could be gained. The lack of complete sterility in this experiment also contributed to bacterial and/or yeast contaminations that increased the original turbidity. The lowest concentration of karo only culture finally turned turbid just yesterday, indicating that it had finally succumbed to contamination. The higher sugar concentrations were able to fend off contams and remain relatively clear. When I poured out the cultures in my backyard grave site, I mostly smelled whatever nutrient was used (V8, rye, soil, etc.), although two or three smelled yeasty, so I suspect yeast contam in those. None had a sour or acrid smell. (By the way, don't go sniffing nasty cultures, the bacteria can take hold in your lungs and even kill you.)
I look forward to a much improved experiment with better sterility, varied nutrient concentrations, and higher clarity of the broth. I was surprised in this experiment how fast the apple/potato broth took off (although it contam'd very fast, also). I was also surprised that the highest and lowest karo concentration showed good growth, I expected only the concentrations near 4% to do well.
That's it for this experiment, I nocced four quarts of rye with the 24% karo culture, and one quart of rye with the karo/rye culture. If anything unusual about the spawn should happen, I will report back.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Liquid culture parameters [Re: mogur]
#5347256 - 02/27/06 09:29 PM (17 years, 10 months ago) |
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20% canned/bottled apple juice to 80% water, has worked well, for me. Numerous times.
 Above is 10% flat beer, 10% apple juice & 80% water. That is one thick mamma jamma LC.
Suggest next time, you use jars, with tubing filter lids.
  
 Beer, apple & cranberry juice LC
Yup, TURBIDITY is a bitch, with any starchy nutrient.
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: Liquid culture parameters [Re: agar]
#5347289 - 02/27/06 09:41 PM (17 years, 10 months ago) |
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Yeah it looks like the 24% Karo kick ass in this one.
Have you came across this Vanilla Extract and Vented lid tek for liquid cultures!
Good luck on the rye, what are you spawning to if you haven't said yet.
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Atheist Chat
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mogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
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Re: Liquid culture parameters [Re: agar]
#5347309 - 02/27/06 09:49 PM (17 years, 10 months ago) |
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Thanks for the input, agar, much appreciated. Yes, being a cheapskate, I've been keeping an eye out for smallish jars without the 'jelly decorations' imprinted in the glass, without having to order from a scientific supply company at 4 bucks a container. And really looking forward to trying your beer and apple juice combo. Also want to try synthetic nutes, and other peroxide compatible ingredients.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Liquid culture parameters [Re: mogur]
#5347358 - 02/27/06 10:01 PM (17 years, 10 months ago) |
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Glass Miricle whip & Mayo quart jars don't have any embossing on them. (frugal agar, LOL)
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mogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
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Re: Liquid culture parameters [Re: Snaggletooth]
#5347542 - 02/27/06 10:38 PM (17 years, 10 months ago) |
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The vanilla tek, yes, saw that. Agar's lids strike again! Bet the corn syrup in the extract didn't hurt.
Don't know yet what I'll spawn the rye to. Probably poo/verm.
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