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shazoom
Al G. ain't no fun guy


Registered: 01/08/06
Posts: 82
Loc: California
Last seen: 6 years, 7 months
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24+hrs. @ 100 are they lost?
#5271576 - 02/06/06 10:02 PM (17 years, 11 months ago) |
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I just checked my incubator and the temp was at least 100 They are B+ 48 hrs old, no sign of spawn - are they ruined?
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gourmetgrower
I'ze the milkmanwhat knocked upyer maw

Registered: 01/29/06 
Posts: 170
Loc: The Old West
Last seen: 17 years, 10 months
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Re: 24+hrs. @ 100 are they lost? [Re: shazoom]
#5271614 - 02/06/06 10:08 PM (17 years, 11 months ago) |
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Make sure that you remove what you are growing from the heat! Check to see if it is dried out. If so, you can inject some sterile water to rehydrate.
If you have any more of what you used to inoculate, you could re-inoculate, just to be sure.
I would say that there is a good chance that your inoculate was killed, but this depends on the volume of substrate. Good luck.
-------------------- Howdy, boys! Let's get down to business. I got my colt and my schofield, and they's jist itchin to be broken in.
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Dribblesmeer
explorer

Registered: 07/17/05
Posts: 88
Loc: Uranus
Last seen: 15 years, 5 months
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Re: 24+hrs. @ 100 are they lost? [Re: gourmetgrower]
#5271622 - 02/06/06 10:09 PM (17 years, 11 months ago) |
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Thermal death happens around 106 so u might b ok. Just try to get your temp back down to about 82
-------------------- Shroomites often have to refrain from the temptation to dose their straight friends, since dosing anyone without their permission is just not considered ethical behavior among psychonauts.
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gourmetgrower
I'ze the milkmanwhat knocked upyer maw

Registered: 01/29/06 
Posts: 170
Loc: The Old West
Last seen: 17 years, 10 months
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Re: 24+hrs. @ 100 are they lost? [Re: gourmetgrower]
#5271632 - 02/06/06 10:11 PM (17 years, 11 months ago) |
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Oh yeah, Paul Stamets establishes a thermal death limit of 106F. If the center of the substrate (not necessarily the incubator) reached 106, then your inoculate has died.
Edit: Sorry for the duplicate post about thermal death.
Edited by gourmetgrower (02/06/06 10:12 PM)
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Dribblesmeer
explorer

Registered: 07/17/05
Posts: 88
Loc: Uranus
Last seen: 15 years, 5 months
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Re: 24+hrs. @ 100 are they lost? [Re: Dribblesmeer]
#5271639 - 02/06/06 10:12 PM (17 years, 11 months ago) |
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also don't jump to any conclutions my first grow didn't show any signs of growth till about 9 days went by. Remember patience is the key!
-------------------- Shroomites often have to refrain from the temptation to dose their straight friends, since dosing anyone without their permission is just not considered ethical behavior among psychonauts.
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Rahz
Alive Again


Registered: 11/10/05
Posts: 9,230
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Re: 24+hrs. @ 100 are they lost? [Re: Dribblesmeer]
#5271794 - 02/06/06 10:47 PM (17 years, 11 months ago) |
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106 is for mycelium right? What is thermal death for spores? I'm not even sure if there would be un-germinated spores after 48 hours, or what temps would affect hydrated spores as opposed to dry spores.
Rahz
-------------------- rahz comfort pleasure power love truth awareness peace "You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi
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coda
Banjo Goiter


Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
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Re: 24+hrs. @ 100 are they lost? [Re: gourmetgrower]
#5271810 - 02/06/06 10:52 PM (17 years, 11 months ago) |
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never rehydrate your cakes, more often then not you will end up with contams because of it.
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
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HippieChick
Chicks can do it too!


Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 13 days
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Re: 24+hrs. @ 100 are they lost? [Re: shazoom]
#5271861 - 02/06/06 11:11 PM (17 years, 11 months ago) |
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If it was multi-spore inoculant , you're fine .
I believe a spore syringe is OK to about 122 degrees F .
Think about it . How hot does it get in the summer when syringes are shipped ? You should be fine . 
Peace,Love,Happiness and Harmony Hippie Chick
-------------------- Peace,Love and Happiness HC Freedoms just another word for nothing left to lose.............. I LUV My Greenhouse http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848 My First Pans http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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lardnar
Pu Pu Platter


Registered: 06/15/05
Posts: 703
Loc: Behind what appears
Last seen: 16 years, 11 months
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Re: 24+hrs. @ 100 are they lost? [Re: HippieChick]
#5272098 - 02/07/06 12:42 AM (17 years, 11 months ago) |
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think about the heat in thailand etc where the spores are natural
-------------------- If your soul is sence this life is lost ...
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: 24+hrs. @ 100 are they lost? [Re: HippieChick]
#5272347 - 02/07/06 05:41 AM (17 years, 11 months ago) |
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Yep 122F. (50C) is where thermodeath limits are for spores, 106F. for myc. Now remember this is where death begins it's the duration at these temps that makes the difference.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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gourmetgrower
I'ze the milkmanwhat knocked upyer maw

Registered: 01/29/06 
Posts: 170
Loc: The Old West
Last seen: 17 years, 10 months
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Re: 24+hrs. @ 100 are they lost? [Re: hyphae]
#5274157 - 02/07/06 04:59 PM (17 years, 11 months ago) |
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I did not know there was a difference for spores and mycelium, but it should have been obvious to me.  Sorry.
-------------------- Howdy, boys! Let's get down to business. I got my colt and my schofield, and they's jist itchin to be broken in.
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shazoom
Al G. ain't no fun guy


Registered: 01/08/06
Posts: 82
Loc: California
Last seen: 6 years, 7 months
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Re: 24+hrs. @ 100 are they lost? [Re: gourmetgrower]
#5274719 - 02/07/06 07:05 PM (17 years, 11 months ago) |
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I see spawn grouth today so they made it. Thanks for all the input.
-------------------- " This hobby teaches us patience, and this community teaches us to share." ~Shazoom
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