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Offlineshazoom
Al G. ain't no fun guy
Male

Registered: 01/08/06
Posts: 82
Loc: California Flag
Last seen: 6 years, 7 months
24+hrs. @ 100 are they lost?
    #5271576 - 02/06/06 10:02 PM (17 years, 11 months ago)

I just checked my incubator and the temp was at least 100
They are B+ 48 hrs old, no sign of spawn - are they ruined? :blush:


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Offlinegourmetgrower
I'ze the milkmanwhat knocked upyer maw

Registered: 01/29/06 Happy 18th Shroomiversary!
Posts: 170
Loc: The Old West
Last seen: 17 years, 10 months
Re: 24+hrs. @ 100 are they lost? [Re: shazoom]
    #5271614 - 02/06/06 10:08 PM (17 years, 11 months ago)

Make sure that you remove what you are growing from the heat! Check to see if it is dried out. If so, you can inject some sterile water to rehydrate.

If you have any more of what you used to inoculate, you could re-inoculate, just to be sure.

I would say that there is a good chance that your inoculate was killed, but this depends on the volume of substrate.
Good luck.


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Howdy, boys! Let's get down to business. I got my colt and my schofield, and they's jist itchin to be broken in.


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OfflineDribblesmeer
explorer
Male
Registered: 07/17/05
Posts: 88
Loc: Uranus
Last seen: 15 years, 5 months
Re: 24+hrs. @ 100 are they lost? [Re: gourmetgrower]
    #5271622 - 02/06/06 10:09 PM (17 years, 11 months ago)

Thermal death happens around 106 so u might b ok. Just try to get your temp back down to about 82


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Shroomites often have to refrain from the temptation to dose their straight friends, since dosing anyone without their permission is just not considered ethical behavior among psychonauts.


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Offlinegourmetgrower
I'ze the milkmanwhat knocked upyer maw

Registered: 01/29/06 Happy 18th Shroomiversary!
Posts: 170
Loc: The Old West
Last seen: 17 years, 10 months
Re: 24+hrs. @ 100 are they lost? [Re: gourmetgrower]
    #5271632 - 02/06/06 10:11 PM (17 years, 11 months ago)

Oh yeah, Paul Stamets establishes a thermal death limit of 106F. If the center of the substrate (not necessarily the incubator) reached 106, then your inoculate has died.

Edit: Sorry for the duplicate post about thermal death.


Edited by gourmetgrower (02/06/06 10:12 PM)


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OfflineDribblesmeer
explorer
Male
Registered: 07/17/05
Posts: 88
Loc: Uranus
Last seen: 15 years, 5 months
Re: 24+hrs. @ 100 are they lost? [Re: Dribblesmeer]
    #5271639 - 02/06/06 10:12 PM (17 years, 11 months ago)

also don't jump to any conclutions my first grow didn't show any signs of growth till about 9 days went by.  Remember patience is the key! :gethigh:


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Shroomites often have to refrain from the temptation to dose their straight friends, since dosing anyone without their permission is just not considered ethical behavior among psychonauts.


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InvisibleRahz
Alive Again
Male

Registered: 11/10/05
Posts: 9,230
Re: 24+hrs. @ 100 are they lost? [Re: Dribblesmeer]
    #5271794 - 02/06/06 10:47 PM (17 years, 11 months ago)

106 is for mycelium right? What is thermal death for spores? I'm not even sure if there would be un-germinated spores after 48 hours, or what temps would affect hydrated spores as opposed to dry spores.

Rahz


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rahz

comfort pleasure power love truth awareness peace


"You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi


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Offlinecoda
Banjo Goiter
Male

Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
Trusted Cultivator
Re: 24+hrs. @ 100 are they lost? [Re: gourmetgrower]
    #5271810 - 02/06/06 10:52 PM (17 years, 11 months ago)

never rehydrate your cakes, more often then not you will end up with contams because of it.


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To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


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OfflineHippieChick
Chicks can do it too!
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Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 13 days
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Re: 24+hrs. @ 100 are they lost? [Re: shazoom]
    #5271861 - 02/06/06 11:11 PM (17 years, 11 months ago)

If it was multi-spore inoculant , you're fine .

I believe a spore syringe is OK to about 122 degrees F .

Think about it . How hot does it get in the summer when syringes are shipped ? You should be fine . :grin:

Peace,Love,Happiness and Harmony
:heart: Hippie Chick  :mushroom2:


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Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
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Offlinelardnar
Pu Pu Platter
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Registered: 06/15/05
Posts: 703
Loc: Behind what appears
Last seen: 16 years, 11 months
Re: 24+hrs. @ 100 are they lost? [Re: HippieChick]
    #5272098 - 02/07/06 12:42 AM (17 years, 11 months ago)

think about the heat in thailand etc where the spores are natural


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If your soul is sence this life is lost ...


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: 24+hrs. @ 100 are they lost? [Re: HippieChick]
    #5272347 - 02/07/06 05:41 AM (17 years, 11 months ago)

Yep 122F. (50C) is where thermodeath limits are for spores, 106F. for myc. Now remember this is where death begins it's the duration at these temps that makes the difference. :wink:


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinegourmetgrower
I'ze the milkmanwhat knocked upyer maw

Registered: 01/29/06 Happy 18th Shroomiversary!
Posts: 170
Loc: The Old West
Last seen: 17 years, 10 months
Re: 24+hrs. @ 100 are they lost? [Re: hyphae]
    #5274157 - 02/07/06 04:59 PM (17 years, 11 months ago)

I did not know there was a difference for spores and mycelium, but it should have been obvious to me. :banghead:
Sorry.


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Howdy, boys! Let's get down to business. I got my colt and my schofield, and they's jist itchin to be broken in.


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Offlineshazoom
Al G. ain't no fun guy
Male

Registered: 01/08/06
Posts: 82
Loc: California Flag
Last seen: 6 years, 7 months
Re: 24+hrs. @ 100 are they lost? [Re: gourmetgrower]
    #5274719 - 02/07/06 07:05 PM (17 years, 11 months ago)

I see spawn grouth today so they made it.
Thanks for all the input.


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" This hobby teaches us patience, and this community teaches us to share."
~Shazoom


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