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Offlinefungiamongi2
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Trouble w/ S. Africa Transkei (broken glass in water jar)
    #5265523 - 02/05/06 08:54 AM (17 years, 11 months ago)

okay, so, usually I use colonized Thailand Koh Samui Super Strain on WBS, take a chunk of it with a spoon, put it in a half pint w/ broken glass and water, shake to knock mycelium into the water, suck up syringes and use this to inoculate my wbs jars. This has always worked for me. Now, i used south africa transkei for my last batch, wbs prepared the same as always, and I have no growth at all after a week. advice? maybe that method works with some strains and not others


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Invisiblemskip23
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Re: Trouble w/ S. Africa Transkei (broken glass in water jar) [Re: fungiamongi2]
    #5265565 - 02/05/06 09:31 AM (17 years, 11 months ago)

what your temps at


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Re: Trouble w/ S. Africa Transkei (broken glass in water jar) [Re: fungiamongi2]
    #5265654 - 02/05/06 10:19 AM (17 years, 11 months ago)

Why don't you just do a straight grain to grain transfer and skip the syringe?


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Offlinefungiamongi2
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Re: Trouble w/ S. Africa Transkei (broken glass in water jar) [Re: FooMan]
    #5265714 - 02/05/06 10:43 AM (17 years, 11 months ago)

temps at 80. I havent tried grain to grain yet b/c I havent built a glovebox yet.


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InvisiblePremedman1
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Re: Trouble w/ S. Africa Transkei (broken glass in water jar) [Re: fungiamongi2]
    #5265773 - 02/05/06 11:08 AM (17 years, 11 months ago)

You don't need a GB for GTG. Be extra sterile. Oust and bleach EVERYTHING. Sterilize your utensils you'll be using between each transfer. Be as quick as possible. I just transferred 4 pints of colonized WBS to 20 quarts with no contams and no GB. A small closet works great for a work area.


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InvisiblePrisoner#1
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Re: Trouble w/ S. Africa Transkei (broken glass in water jar) [Re: Premedman1]
    #5266100 - 02/05/06 12:44 PM (17 years, 11 months ago)

Quote:

Premedman1 said:
You don't need a GB for GTG. Be extra sterile.





being extra sterile requires a flow hood or glovebox, open air transfers have a
risk, oust, bleach and what ever dont actualy sanitize the air, and as cheap
as it is to make a box for transfers, it's pointless not to.


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InvisiblePremedman1
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Re: Trouble w/ S. Africa Transkei (broken glass in water jar) [Re: Prisoner#1]
    #5266361 - 02/05/06 01:45 PM (17 years, 11 months ago)

Quote:

Prisoner#1 said:
being extra sterile requires a flow hood or glovebox




I guess you're right. I suppose I just got very lucky the past 3 times I have done this with 10-15 quart jars per run with no contams and no flowhood/glovebox. I'd love to have a flowhood, but the girlfriend's not to keen on turning our kitchen into a lab. Whatever works for you man. :wink:


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InvisiblePrisoner#1
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Re: Trouble w/ S. Africa Transkei (broken glass in water jar) [Re: Premedman1]
    #5266385 - 02/05/06 01:50 PM (17 years, 11 months ago)

luck may play a small part but good technique is also a major factor, people
have been known to get contams even with flowhoods because they have no
real concern about the processes...


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Offlinefungiamongi2
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Re: Trouble w/ S. Africa Transkei (broken glass in water jar) [Re: Prisoner#1]
    #5266553 - 02/05/06 02:50 PM (17 years, 11 months ago)

yeah, i want to make a glovebox and do grain to grains, but thats out of the question for a couple of weeks. Ive always had success before with the broken glass in a jar, so i was just trying to repeat that until i build a gb. im still trying to see if anybody has had success using this technique for some strains and not others


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