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OfflineBMArts
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Registered: 01/07/06
Posts: 215
Last seen: 16 years, 11 months
Shake your Syringes!!!! (grrr)
    #5265300 - 02/05/06 03:52 AM (17 years, 11 months ago)

Ok ok... well. Stupid mistake I made.
Its now been a week and a day since I injected 1cc into my first LC (honey water tek). Nothing seemed to be growing so 2 days ago I tried another one with malt/dextrose.
Well now after 2 days I can see the myc starting in the new one whereas in the old one up to now (as far as I can tell) there is only one little fluffbit that I think is myc.
I realized right after I had injected that I had forgotten to shake, but I thought it might work anyhow.. well that was definetly the problem... stupid stupid me


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Everything I post on this board is pure fiction. Nothing in the post above is real. It is all made up...


May the source be with GNU


Edited by BMArts (02/05/06 09:56 AM)


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Offlinedjsage420
aka DJQBNSIS
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Registered: 02/15/05
Posts: 869
Loc: Panhandle
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Re: Shake your Syringes!!!! (grrr) [Re: BMArts]
    #5265382 - 02/05/06 06:59 AM (17 years, 11 months ago)

Your not going to see much growth after 2 days, thats barely enough time for spores to germinate let alone grow. Liquid cultures dont do well if they are constantly being handled and looked at, let it inccubate for a week then look at it.
I dont think shaking the syringe had a big effect, there are millions of spores in every syringe. Carmelizing the honey will damper the growth though


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If you put your head to the grass, you can hear it growing


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Offlinerawtoxic
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Registered: 10/06/02
Posts: 2,097
Loc: smokey mountains
Last seen: 13 years, 5 months
Re: Shake your Syringes!!!! (grrr) [Re: djsage420]
    #5265484 - 02/05/06 08:35 AM (17 years, 11 months ago)

You will find, with LC syringes I think shaking is more important becasue especially if syringes have been stored a while all mycelium will clump together.

Most LCs from spore won't show very noticeable growth for 72 hours, don't even check them until then. I will agree that handling LC jars does not help them especially in early stages of growth.

Just get some other things to do with your time and those days go by like minutes.

Like grow some nugs or something.

Edit:Typo


Edited by rawtoxic (02/05/06 10:05 AM)


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OfflineBMArts
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Registered: 01/07/06
Posts: 215
Last seen: 16 years, 11 months
Re: Shake your Syringes!!!! (grrr) [Re: rawtoxic]
    #5265602 - 02/05/06 09:59 AM (17 years, 11 months ago)

aha... ok now I'm confused. I guess ignore my post then. Crap perhaps its contaminated then... ok.
But I do read everywhere to agitate once a day... thats all I've really been doing. Not checking often or anything... hmm anyhow forget about it. thanks guys


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Everything I post on this board is pure fiction. Nothing in the post above is real. It is all made up...


May the source be with GNU


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Offlinehyphae
born to grow
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Registered: 12/13/02
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Re: Shake your Syringes!!!! (grrr) [Re: BMArts]
    #5265737 - 02/05/06 10:53 AM (17 years, 11 months ago)

Shaking is always imporant it's basic Myco 101! Spores clump (fact) shaking evenly distributes the spores in the syringe so all he jars get an even amount, not shaking can often times leave you with totally uncolonized jars. Like they said give it a week and have patients.


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
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Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineBMArts
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Registered: 01/07/06
Posts: 215
Last seen: 16 years, 11 months
Re: Shake your Syringes!!!! (grrr) [Re: hyphae]
    #5268938 - 02/06/06 10:22 AM (17 years, 11 months ago)

Well I don't know whats up... its now the third day since inoculation of my Malt/Dextrose LC (with spores) and there are now a nummber of myc blobs floating around in it (the biggest about the size of the tip of my little finger). I only know this because I take it out once a day to shake.
I am sure now that the what I saw yesterday were the same blobs just smaller. Now because someone higher up said I should see noticable growth until after 72 hours but now I see very noticable growth (and did a bit yesterday as well.. which was 2 days after inoculation) I am a bit concerned as to wether this is contamination...
Anyone who can give me hints as to wether or not it could be?!?!
Thanks a lot


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Everything I post on this board is pure fiction. Nothing in the post above is real. It is all made up...


May the source be with GNU


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InvisibleFooMan
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Registered: 02/02/05
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Re: Shake your Syringes!!!! (grrr) [Re: BMArts]
    #5268956 - 02/06/06 10:31 AM (17 years, 11 months ago)

Pictures Help.


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Quick WBS Prep


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OfflineBMArts
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Registered: 01/07/06
Posts: 215
Last seen: 16 years, 11 months
Re: Shake your Syringes!!!! (grrr) [Re: FooMan]
    #5268975 - 02/06/06 10:43 AM (17 years, 11 months ago)

And having a digital camera would would help for that... sorry *shrugz*


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Everything I post on this board is pure fiction. Nothing in the post above is real. It is all made up...


May the source be with GNU


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