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OfflineClearWhiteLight
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Registered: 12/30/05
Posts: 2
Last seen: 18 years, 10 months
question about casing
    #5261387 - 02/04/06 12:37 AM (18 years, 11 months ago)

Pretty soon I'll be beginning my second attempt to grow some mushrooms. I was wondering if it was nessicary to prepare casings inside a glovebox.. I'll be doing my inoculation inside one, but its too small to prepare the casings with.


Is there much risk of contamination when preparing casings in open air? BTW I have a pressure cooker so I will be sterilizing my casing materials(vermiculute/coco coir), im just wondering whether I should bother with trying to build a much larger glovebox that I could prepare numerous casings inside of, or if its unnessicary.

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InvisiblePremedman1
Assistant to the insistent
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Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Re: question about casing [Re: ClearWhiteLight]
    #5261396 - 02/04/06 12:43 AM (18 years, 11 months ago)

No, a GB is not necessary for casing. Fully colonized mycelium is very resistant to contams. I wouldn't crumble it up and watch the game before adding my casing layer or anything. Just wash up real well, thoroughly clean your utensils you'll be using, and you'll be fine.


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Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.

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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: question about casing [Re: Premedman1]
    #5262499 - 02/04/06 12:55 PM (18 years, 11 months ago)

Quote:

Premedman1 said:
No, a GB is not necessary for casing. Fully colonized mycelium is very resistant to contams. I wouldn't crumble it up and watch the game before adding my casing layer or anything. Just wash up real well, thoroughly clean your utensils you'll be using, and you'll be fine.



Agreed also it doesn't hurt to spray down the room beforehand with Lysol or Oust, you want to be clean about things in any case.


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Getting the most out of your casings!, A pinning strategy.
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"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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