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OfflineJSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 2 months, 15 days
jars not colonizing?
    #5259901 - 02/03/06 04:36 PM (17 years, 11 months ago)

I made 14 qts of wbs the agar method with a 24 hr ish soak. no seed germination. Polyfil filters. Inoculated with 5-10 cc of LC. it wasnt foggy but definetly there. I did forget to shake the jars out of the PC. They do not look too wet or dry. Just nothing? any ideas


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First Grow No more carpet tek


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Offlinekiss
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Registered: 12/14/04
Posts: 486
Loc: under your bed
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Re: jars not colonizing? [Re: JSshroom]
    #5259917 - 02/03/06 04:40 PM (17 years, 11 months ago)

Did you rinse the seed?


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see dick drink
see dick drive
see dick die
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OfflineJSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 2 months, 15 days
Re: jars not colonizing? [Re: kiss]
    #5266907 - 02/05/06 05:34 PM (17 years, 11 months ago)

No I did not. there are a # of threads without it being neccessary? scatmanrav and the like do not. ?


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First Grow No more carpet tek


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OfflineFruitboot
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Registered: 10/04/05
Posts: 417
Last seen: 12 years, 23 days
Re: jars not colonizing? [Re: JSshroom]
    #5266978 - 02/05/06 06:15 PM (17 years, 11 months ago)

How long has it been?


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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
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Re: jars not colonizing? [Re: JSshroom]
    #5267439 - 02/05/06 08:52 PM (17 years, 11 months ago)

Were the WBS jars cooled overnight to room temps, pre/inoculation?

Did you shake the jars after you inoculated?

How long has it been since you innoc'ed?


50 hours after LC innoc.


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InvisibleJaeger
Dreamer
Registered: 10/01/05
Posts: 960
Re: jars not colonizing? [Re: agar]
    #5267565 - 02/05/06 09:14 PM (17 years, 11 months ago)

Does the seed have a coating on it?
That can be very bad for mycel. growth :smile:


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OfflineJSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 2 months, 15 days
Re: jars not colonizing? [Re: Jaeger]
    #5267810 - 02/05/06 10:37 PM (17 years, 11 months ago)

I do not know about a coating? it is the same seed I used for my other thread. I did let them cool overnight. its been 5 days. For a LC that is way slow it would seem. yes I lightly shook the jars.


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First Grow No more carpet tek


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InvisibleJaeger
Dreamer
Registered: 10/01/05
Posts: 960
Re: jars not colonizing? [Re: JSshroom]
    #5267826 - 02/05/06 10:41 PM (17 years, 11 months ago)

I knew a guy who tried some WBS with BirdKote, he made up 20 jars, tried a few different ways to innoc, none worked.  Not even a beautiful LC he had.
So, he bought another type of WBS, preped jar same way, and innoc'd with the LC, bam growth in a day.  I would go with a cheap WBS, like agar generally recommends, a good rule of thumb would be to read the ingredients and make sure there are only seeds, and maybe a buffer for ph :smile:
Cheaper too, $8 for 30-40lbs

EDIT: Nice first grow, 5 for that baby!!!  :laugh:


Edited by Jaeger (02/05/06 10:43 PM)


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OfflineJSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 2 months, 15 days
Re: jars not colonizing? [Re: Jaeger]
    #5278714 - 02/08/06 06:33 PM (17 years, 11 months ago)

this wbs is the expensive Pennington brand in the yellow bag. it has more millet then the cheap stuff.

also my last run all the wbs jars were simmered and rinsed. They were also all inoculated with g2g.

any ideas folks


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First Grow No more carpet tek


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InvisibleSwan Song
Registered: 02/08/06
Posts: 559
Re: jars not colonizing? [Re: JSshroom]
    #5278778 - 02/08/06 06:47 PM (17 years, 11 months ago)

tell me what brand name the seed was so i can avoid it.


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Offlinehyphae
born to grow
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Re: jars not colonizing? [Re: Swan Song]
    #5279574 - 02/08/06 09:55 PM (17 years, 11 months ago)

Alot (most) of brands have fungicides added to prevent molds and prolong storage, they all need to be rinsed throughly it's just mycosense IME. Moisture content is also always a biggie too with any substrate. GL


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OfflineJSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 2 months, 15 days
Re: jars not colonizing? [Re: hyphae]
    #5282389 - 02/09/06 04:10 PM (17 years, 11 months ago)

Pennington brand in the yellow bag. are there any names on the bag to look for IE..fungicides and what not. This stuff worked once before. I am gonna do 7 qts simmered and rinsed and do 7 soaked and rinsed. Knock up with the same LC and see what happens


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First Grow No more carpet tek


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