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CubensisCutter
mycologist


Registered: 07/04/05
Posts: 1,775
Last seen: 8 years, 8 months
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FAE for incubating jars?
#5255474 - 02/02/06 02:01 PM (17 years, 11 months ago) |
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is this important?? i have 4 1/2 pint BRF cakes incubating iside a tiny little carboard box that just barely fits them and i put a heating pad ovr the box on low temp and there is already white stuff starting to take over the jars in only 1 week (im hoping its myc, not a contam) i open the box about twice a day to air it out for a couple minutes... should i do this more often or does in not matter. (the temp in the box is in the upper 70s, low 80s)
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thats right cubes in december bitches
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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No, FAE really isn't all that important for colonizing jars. There will be gas exchange occuring, but nothing substantial enough to merit fanning your incubator.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Yep no fanning besides the jars are mostly sealed to hold moisture in anyways. You do not want the jars of course to be totally sealed as that would not allow gas exchange but you've most likely read that already right?
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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WhiteBunny
How deep doesthe rabbit hole go?


Registered: 07/29/05
Posts: 1,351
Loc: Earth
Last seen: 15 years, 4 months
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Re: FAE for incubating jars? [Re: hyphae]
#5256753 - 02/02/06 07:40 PM (17 years, 11 months ago) |
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I would take the foil tops off and flip if they stalled.
WB
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CubensisCutter
mycologist


Registered: 07/04/05
Posts: 1,775
Last seen: 8 years, 8 months
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Re: FAE for incubating jars? [Re: WhiteBunny]
#5256878 - 02/02/06 08:08 PM (17 years, 11 months ago) |
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Quote:
WhiteBunny said: I would take the foil tops off and flip if they stalled.
WB
please explain?
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thats right cubes in december bitches
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Premedman1
Assistant to the insistent


Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Quote:
CubensisCutter said:
Quote:
WhiteBunny said: I would take the foil tops off and flip if they stalled.
WB
please explain?
WB's just saying that if colonization slows down or stops due to poor gas exchange, flipping your jars upside-down can help to reinitiate growth. This idea comes from the fact that CO2 is heavier than O2, and will be expelled from the jar through your hole in the lid.
-------------------- Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
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DIRTYMAN
Jesusdon'tcomethrough thecotton.

Registered: 11/18/05
Posts: 18,558
Loc: CZ NIGGUH
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Re: FAE for incubating jars? [Re: Premedman1]
#5257465 - 02/02/06 10:13 PM (17 years, 11 months ago) |
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What if one used a G2G transfer and closed their lids tight? Could that hinder the incubation process? I was wondering why it was taking so long...
-------------------- I'm racist. http://k-k-k.com/
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CubensisCutter
mycologist


Registered: 07/04/05
Posts: 1,775
Last seen: 8 years, 8 months
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Re: FAE for incubating jars? [Re: Premedman1]
#5257466 - 02/02/06 10:13 PM (17 years, 11 months ago) |
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there are no holes in my lid. just the 4 that were punched in the lid so i could inoculate, but they are covered with tape right now...should i have another hole?
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thats right cubes in december bitches
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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No, just take the tape off. You do have the inch layer of vermiculite on top, right? If you do, then just take the tape off. If you don't.. I'd try to find some micropore tape that will let your jars breathe.
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CubensisCutter
mycologist


Registered: 07/04/05
Posts: 1,775
Last seen: 8 years, 8 months
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yeah i have the verm...the PF Tek said to put the tape back on before incubating....so is it better if i take it off?
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thats right cubes in december bitches
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Yeah, so long as you've got that verm there it will act as a filter, allowing gas exchange and keeping the nasties out
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phantomstranger
Stranger

Registered: 12/17/05
Posts: 285
Last seen: 6 years, 2 months
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Just take the tape and/or foil off, it wont hurt and it may even help speed up colonization or at least keep them from stalling. The dry layer of verm is used to prevent contams from getting inside your jars.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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That if one flaw with the PFTek putting the tape back on, it's totally unnecessary and makes it easy for many to forget. The instructions most definitely needs to be updated (changed)(fine tuned)(etc;). Gas exchange is needed for healthy cultures!
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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