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mrwacko15
Stranger
Registered: 12/28/05
Posts: 9
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Humidity while colonizing
#5253827 - 02/02/06 12:37 AM (18 years, 3 hours ago) |
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I have a BRF grow going, and I was wondering if having high humidity in the chamber where my jars are colonizing would slow the process at all, or if it doesn't really matter. I know that I need high humidity for the fruiting stage, but I plan to use the same setup for both colonization and fruiting.
Also, would it create any problems if I cased colonized BRF and colonized WBS in the same container? I only used 1/2 pint jars and won't end up with all that much substrate, so it'd help out if I could combine them.
Thanks for any help you could give me.
Edited by mrwacko15 (02/02/06 12:50 AM)
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Beebo
Stranger


Registered: 01/17/06
Posts: 197
Last seen: 14 years, 4 months
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Re: Humidity while colonizing [Re: mrwacko15]
#5253876 - 02/02/06 01:03 AM (18 years, 3 hours ago) |
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Well, the real problem I see is that there's no real way for the humidity in the air to get to your cakes beause of the jars. Perhaps through the holes in the top, but wetting the layer of vermicullite could cause contamination
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Re: Humidity while colonizing [Re: Beebo]
#5253894 - 02/02/06 01:11 AM (18 years, 3 hours ago) |
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I would speculate that having humid, stagnant air in an incubator would promote an environment welcoming contams to take hold on your cakes before the mycelium is strong enough to take hold.
But the main question is, even though you're using the same chamber for incubating and fruiting, why the hell would you saturate the perlite/lava rocks when it's only in the incubation phase? Just wait to supply the humidity source until you're ready to birth the cakes. And if you're asking because you want to keep colonizing jars inside the FC while fully colonized cakes are fruiting, then I'm going to have to frown upon that idea and suggest that you just incubate on a bookshelf at room temp.
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Oh, and mixing spawn/substrate is just fine. In the end, it's all just food for the mycelium and mushrooms.
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Rahz
Alive Again


Registered: 11/10/05
Posts: 9,230
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Re: Humidity while colonizing [Re: mrwacko15]
#5253914 - 02/02/06 01:21 AM (18 years, 2 hours ago) |
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Like rabid_birds said, you'd have to fan your jars every day. Just let them colonize outside the FC, or remove the humidity.
Rahz
-------------------- rahz comfort pleasure power love truth awareness peace "You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Humidity while colonizing [Re: Rahz]
#5254146 - 02/02/06 05:47 AM (17 years, 11 months ago) |
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I have often used humid incubators when using bags as well as jars with filter patches. This has prevented the grains from drying out on the surface believe it or not and works extremely well with slow colonizers (cold loving species).
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Rahz
Alive Again


Registered: 11/10/05
Posts: 9,230
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Re: Humidity while colonizing [Re: hyphae]
#5254228 - 02/02/06 07:21 AM (17 years, 11 months ago) |
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Yep, I think I've read the optimal RH for colonization is 90, but I would be worried about FAE. It might be worth it, I just grinned at the thought of fanning jars.
Rahz
-------------------- rahz comfort pleasure power love truth awareness peace "You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Humidity while colonizing [Re: Rahz]
#5254802 - 02/02/06 11:27 AM (17 years, 11 months ago) |
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Quote:
Rahz said: Yep, I think I've read the optimal RH for colonization is 90, but I would be worried about FAE. It might be worth it, I just grinned at the thought of fanning jars.
Rahz
Actually myc colonizes fastest at near saturation (98%+). Absolutely no FAE is wanted during colonization but gas exchange is essential.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Rahz
Alive Again


Registered: 11/10/05
Posts: 9,230
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Re: Humidity while colonizing [Re: hyphae]
#5255235 - 02/02/06 01:03 PM (17 years, 11 months ago) |
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Stamets recommends 90% for the spawn run in The Mushroom Cultivator, he doesn't list incubation RH, I assume that's since they're in jars and the RH inside the jars would naturally be high. My only concern would be stagnant humid air surrounding my jars, especially if they were PF and boiled, and incubated for a month. It might be fine, but I'm paranoid of contams. If he's got perlite or something in there, it would be a concern for me.
Rahz
-------------------- rahz comfort pleasure power love truth awareness peace "You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi
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LochRaven
mycophile


Registered: 01/31/06
Posts: 190
Last seen: 13 years, 12 days
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Re: Humidity while colonizing [Re: Rahz]
#5255294 - 02/02/06 01:18 PM (17 years, 11 months ago) |
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for my first cakes i used the same chamber... i just didn't humidify it while incubating. this worked fine. and is good when there is limited space
-------------------- there are rainbows and earthworms and microscopic hummingbirds www.elestialsound.com
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