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OfflineDrone
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Loc: Maryland
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LC - time for first signs of growth
    #5252016 - 02/01/06 05:14 PM (18 years, 14 hours ago)

PC'd 200ml of honey water for 20 min of 15psi, inoculated in a glovebox with a fresh syringe. the water was not carmelized. looked the same before and after PC. Probly the color of a very light beer.

its been 6 days in an TiT incubator at 82F. I dont really see anything much going on in the jar. I can barely see some stuff that reminds me of dust floating in the air, but its looked the same for the last couple days.

How long does it normally take to see definate growth.


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Edited by Drone (02/02/06 12:49 AM)


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OfflineCray
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Re: LC first growth signs time. [Re: Drone]
    #5252151 - 02/01/06 05:54 PM (18 years, 13 hours ago)

if it been 6 days, and you see no signs of growth, something went wrong...


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Offlineboosthappyvw
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Registered: 11/22/05
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Re: LC first growth signs time. [Re: Cray]
    #5252191 - 02/01/06 06:06 PM (18 years, 13 hours ago)

if the spores ae fresh 2-3 days if old a week at most so try again.
i never pc my lc i just put some honey in and distilled water in a sterile bottle add a few spores or small shot of lc and in a couplke weeks bingo. i do 4 at a time just in case and have only lost 20 of 20 and it was because of improper handling later on good luck and i just leave them at room temp


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Offlineonetime
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Re: LC first growth signs time. [Re: boosthappyvw]
    #5252259 - 02/01/06 06:23 PM (18 years, 13 hours ago)

I nuked some yesterday nocced it up today I hope to see signs in two days.


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OfflineHoss
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Re: LC first growth signs time. [Re: Drone]
    #5252314 - 02/01/06 06:37 PM (18 years, 13 hours ago)

Quote:

Drone said:
PC'd 200ml of honey water for 20 min of 15psi, inoculated in a glovebox with a fresh syringe. the water was not carmelized. looked the same before and after PC. Probly the color of a very light beer.

its been 6 days in an TiT incubator at 82F. I dont really see anything much going on in the jar. I can barely see some stuff that reminds me of dust floating in the air, but its looked the same for the last couple days.

How long does it normally take to see definate growth.




Exact same thing for me with a Pan. cyan LC that I knocked up last Friday. No signs of growth. Just some stuff that looks like dust in the air.

Hmmm...


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OfflineDrone
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Re: LC first growth signs time. [Re: Hoss]
    #5252332 - 02/01/06 06:44 PM (18 years, 12 hours ago)

im still hoping they are just slow to germinate. it was some packet honey...maybe ill try karo.


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InvisibleOmnicracker
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Re: LC first growth signs time. [Re: boosthappyvw]
    #5252431 - 02/01/06 07:17 PM (18 years, 12 hours ago)

Quote:

boosthappyvw said:
i do 4 at a time just in case and have only lost 20 of 20 and it was because of improper handling




...im convinced.


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Offlineskeletor
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Re: LC first growth signs time. [Re: Omnicracker]
    #5252589 - 02/01/06 08:00 PM (18 years, 11 hours ago)

maybe not enough honey?


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OfflineDrone
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Re: LC first growth signs time. [Re: skeletor]
    #5253845 - 02/02/06 12:48 AM (18 years, 6 hours ago)

i think i had the right amount of honey...now im thinkin perservatives or something added to the honey is the problem.


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Offlinekrill
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Re: LC first growth signs time. [Re: Drone]
    #5253863 - 02/02/06 12:55 AM (18 years, 6 hours ago)

use karo.
i knocked up 12 baby bottles on monday. got growth in all but 2 already. the treasure coasts and ecuadors are in the lead.
karo solution+good, fresh spores=myc in 1-3 days.


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OfflineBMArts
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Re: LC first growth signs time. [Re: krill]
    #5254004 - 02/02/06 02:48 AM (18 years, 4 hours ago)

hmm for me its been 5 days now and I don't see anything either...
Guess I'll start again too


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Offline_OttO_
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Re: LC first growth signs time. [Re: boosthappyvw]
    #5254128 - 02/02/06 05:27 AM (18 years, 2 hours ago)

Quote:

boosthappyvw said:
have only lost 20 of 20




Not very good odds mate - Id start using the pressure cooker if you have one, or at the very least steam sterilise them.


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OfflineHoss
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Re: LC first growth signs time. [Re: _OttO_]
    #5254159 - 02/02/06 05:59 AM (18 years, 1 hour ago)

Well I'm using karo.

I just don't know what's up.


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Offlineblackout
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Re: LC first growth signs time. [Re: Hoss]
    #5254192 - 02/02/06 06:30 AM (18 years, 1 hour ago)

I have had LCs take a month to start.


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OfflineDrone
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Re: LC first growth signs time. [Re: blackout]
    #5254530 - 02/02/06 09:54 AM (17 years, 11 months ago)

well, its been 7 days. I now see a pea sized white fluff floating around. And it looks like a few tiny fluffs are beginning to form as well. These spores were fresh, they were cambos i just got from spores101.

I think most of these teks and posts on LC need to add a few days to the time. Ive been reading almost nothing but 3-5 days for visable growth.

Well im just glad I didnt grow contams. First time using my new Glovebox.


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OfflineDrone
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Re: LC first growth signs time. [Re: Drone]
    #5271313 - 02/06/06 08:47 PM (17 years, 11 months ago)

The little fluff ball grew and grew. When it was about an inch across I shook the jar to break it up into a few pieces, and all of these grew...which i shook into more pieces. It looks very healthy and its almost ready to be used. Im guessing the germination stage took a little bit long. Not everybody can expect to see myc in 3-5 days, even when you do it right.


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OfflineDribblesmeer
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Re: LC first growth signs time. [Re: Hoss]
    #5271367 - 02/06/06 09:05 PM (17 years, 11 months ago)

Quote:

Drone said:

its been 6 days in an TiT incubator at 82F. I dont really see anything much going on in the jar. I can barely see some stuff that reminds me of dust floating in the air, but its looked the same for the last couple days.






Thats how my LC started it looked like dust. It would eventually settle to the bottom but whenever agitated it would go back to looking like dust.  I've knocked up some BRF jars with it and they showed growth after 2 days.  Just let it incubate another week it'll be good to go. :thumbup:


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Shroomites often have to refrain from the temptation to dose their straight friends, since dosing anyone without their permission is just not considered ethical behavior among psychonauts.


Edited by Dribblesmeer (02/06/06 09:14 PM)


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OfflineOatman2000
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Re: LC first growth signs time. [Re: blackout]
    #5271420 - 02/06/06 09:19 PM (17 years, 11 months ago)

Quote:

blackout said:
I have had LCs take a month to start.




only once has that happened to me... got a good faith sent to me in the mail, SA Transki.

BIG DARK print... and when i made my syringe's, i think i didn't chop em up good enough. verry clumpy, and i knocked up LC, and waited....waited, and waited some more.

finally after a month, i am seeing growth. was getting worried for a min.


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OfflineDrone
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Re: LC first growth signs time. [Re: Dribblesmeer]
    #5272103 - 02/07/06 12:44 AM (17 years, 11 months ago)

you made a syringe when it looked like dust? or after u grew it out?


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OfflineDribblesmeer
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Re: LC first growth signs time. [Re: Drone]
    #5272652 - 02/07/06 09:08 AM (17 years, 11 months ago)

Quote:

Drone said:
you made a syringe when it looked like dust? or after u grew it out?




My LC always resembled dust when aggitated with the exception of a few small clumps. It seems to be working fine. Why?


--------------------
Shroomites often have to refrain from the temptation to dose their straight friends, since dosing anyone without their permission is just not considered ethical behavior among psychonauts.


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OfflineMycophreek423
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Re: LC first growth signs time. [Re: Dribblesmeer]
    #5274149 - 02/07/06 04:56 PM (17 years, 11 months ago)

i have made lc before and used it before its time according to others, i still had great results. all i mean is that my lc looked nothing like some i have seen lately, and yes it just kinda looked like dust maybe the biggest thing i could see was a 16th of an inche at best


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Invisibleagar
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Re: LC first growth signs time. [Re: Mycophreek423]
    #5274209 - 02/07/06 05:13 PM (17 years, 11 months ago)




Healthy thick LC's


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