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Offlinerequiem99
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Registered: 10/12/05
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Last seen: 17 years, 4 months
Tell me how bad I fucked my project up.
    #5247426 - 01/31/06 03:56 PM (18 years, 11 hours ago)

I'm following the PFTEK for simple minds as linked to on this site (currently 3 days into incubation and awaiting mycelial growth), with the following changes, all due to necessity and none to my own genius or "invention":

1) I did not use triple foil lids, I used single foil to sterilize the jars and then injected spores and immediately added sterile metal lids (no holes). Foil was sterlized prior to injecting with spores.

2) I sterlized using steam. I know this is iffy but I didn't have access to a pressure cooker OR a place to use it, so I boiled for 30-60 minutes, waited a day, then boiled again for 30-60 minutes a second time. The water ran out a few times and a few of the jars were subjected to dry heat, so this may have altered the water content -- I really don't know if it would since the jars were sealed. Someone can help me out on this one.

3) The spore syringes I used (1 syringer per 6 jars for 12 total) were PR and KS, not the best strains for a newbie but I didn't buy them when I knew what i was doing. That brings me to another thing: They've been kept in storage (a cool drawer sealed in the vacuum pack until 3 weeks ago) for 3 months since I bought them, and have been moved around in a bag to other locations 3 or 4 times. Does this mean I'm SOL and nothing will happen?

Also, do my jars need holes to incubate properly or are they fine as sealed?

I can post pics later on if anyone wants.


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OfflineChronicStiffy
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Re: Tell me how bad I fucked my project up. [Re: requiem99]
    #5247576 - 01/31/06 04:33 PM (18 years, 10 hours ago)

So did you inoculate the jars before or after you sterilized them?

From my understanding, jars don't need FAE but I may be wrong.


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OfflineSnaggletooth
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Re: Tell me how bad I fucked my project up. [Re: ChronicStiffy]
    #5247589 - 01/31/06 04:35 PM (18 years, 10 hours ago)

Jars don't need FAE but they do or should have gas exchange other wise they will stall or can colonize slowly


--------------------


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OfflinePr0_X
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Re: Tell me how bad I fucked my project up. [Re: Snaggletooth]
    #5247616 - 01/31/06 04:42 PM (18 years, 10 hours ago)

No need to worry at all.

I use the steam method to sterlize my PF jars and they turned out fine. I also used a single peice of tinfoil, let the jars cool and then inoculated.

did you add a thin layer of vermiculite to the top of your jars like it says in the tek?
and when boiling your jars, boil on low heat to avoid the pot overflowing and causing a mess, also beware not to burn the buttom of your substrate by placing a teatowel or cloth on the buttom of the pot and then placing the jars ontop of it.

Anywho, your fine man, now comes the waiting game.

Happy growing :smile:


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

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OfflineHengman
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Re: Tell me how bad I fucked my project up. [Re: Snaggletooth]
    #5247620 - 01/31/06 04:43 PM (18 years, 10 hours ago)

was the foil on the inside or the outside of the lid?

from my understanding... if syringes are kept in a dark, cool location, they are still viable up to 3 or 4 months MAX.

jars dont need FAE, but some gas exchange, as snaggletooth said. the best method of gas exchange is polyfill in the lid.

cant help u out on the water content w/out pics. it depends on how long they were exposed to dry heat


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OfflineOrranis
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Re: Tell me how bad I fucked my project up. *DELETED* [Re: Hengman]
    #5247931 - 01/31/06 06:08 PM (18 years, 9 hours ago)

Post deleted by Orranis

Reason for deletion: had to



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Offlinerequiem99
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Re: Tell me how bad I fucked my project up. [Re: Orranis]
    #5248312 - 01/31/06 07:32 PM (18 years, 8 hours ago)

I flamed the needle with a butane lighter AND a regular lighter after EACH jar and held my breath in a fairly sterile environment with as little airflow as possible.

I most certainly used the top layer of verm like the tek says. Thats one of the biggest keys from what I read.

RE FAE: How do I accomplish this? Right now some of my jars have that foggy condensation on them that comes back even if wiped off (weird shit). I have the lids on (screwed on top of the single layer of foil, which has 4 holes in it).


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Offlinerequiem99
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Re: Tell me how bad I fucked my project up. [Re: requiem99]
    #5248377 - 01/31/06 07:43 PM (18 years, 7 hours ago)

As good a pic as I can get (fine grade vermiculite was used as you can tell):



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InvisibleFooMan
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Re: Tell me how bad I fucked my project up. [Re: requiem99]
    #5248432 - 01/31/06 07:56 PM (18 years, 7 hours ago)

Your foil is under a metal lid? If so, you have NO gas exchange and you will have very slow growth or none at all. Poke some holes in the foil if there aren't some already.


--------------------

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Offlinerequiem99
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Re: Tell me how bad I fucked my project up. [Re: FooMan]
    #5248439 - 01/31/06 07:57 PM (18 years, 7 hours ago)

Quote:

FooManShroom said:
Your foil is under a metal lid? If so, you have NO gas exchange and you will have very slow growth or none at all. Poke some holes in the foil if there aren't some already.




There are 3-4 holes per foil lid, but the metal lid is on top of that, screwed on tight. Should it be loosened, or removed?


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OfflineOrranis
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Re: Tell me how bad I fucked my project up. *DELETED* [Re: requiem99]
    #5248868 - 01/31/06 09:31 PM (18 years, 6 hours ago)

Post deleted by Orranis

Reason for deletion: need to



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Life is a pill which none of us can bear to swallow without gilding.


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OfflineOatman2000
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Re: Tell me how bad I fucked my project up. [Re: Orranis]
    #5248930 - 01/31/06 09:44 PM (18 years, 5 hours ago)

the foil is just so water doesn't get in your inoculation holes...


--------------------
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OfflineTriad
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Re: Tell me how bad I fucked my project up. [Re: Orranis]
    #5248940 - 01/31/06 09:47 PM (18 years, 5 hours ago)

He already inoculated, thats a bad idea, mister.


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Offlinecoda
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Re: Tell me how bad I fucked my project up. [Re: Triad]
    #5249141 - 01/31/06 10:24 PM (18 years, 5 hours ago)

you really need to reread that tek man. I dont remember anywhere seeing a part about putting tinfoil over the jar and the lid over that. Remember follow the tek to the LETTER and you'll end up with fruits.


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

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i really am glad you came back to us instead of taking the other path. *hug*

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Offlinerequiem99
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Re: Tell me how bad I fucked my project up. [Re: coda]
    #5250530 - 02/01/06 10:06 AM (17 years, 11 months ago)

Sorry, like I said above, I couldn't follow the tek to the letter. I improvised due to circumstances.


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Offlinerequiem99
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Re: Tell me how bad I fucked my project up. [Re: requiem99]
    #5252465 - 02/01/06 07:26 PM (17 years, 11 months ago)

Well its now day 5, and no mycelium OR mold growth yet. How long will it take to know that nothing is going to grow due to inadequate humidity?


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OfflineMaverick
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Re: Tell me how bad I fucked my project up. [Re: requiem99]
    #5252480 - 02/01/06 07:32 PM (17 years, 11 months ago)

On the bright side, it's not the end of the world and it's a learning experience, right requiem.  :smile:  Just retry and you'll get it!


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Offlineshroomsynergy
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Re: Tell me how bad I fucked my project up. [Re: Maverick]
    #5252508 - 02/01/06 07:41 PM (17 years, 11 months ago)

does the metal lid have holes in it too??? have you tried tyvek as a filter?


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OfflineHypercube
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Re: Tell me how bad I fucked my project up. [Re: Maverick]
    #5252524 - 02/01/06 07:44 PM (17 years, 11 months ago)

Some BRF jars can take a long time to show growth, or don't grow at all. If there's no signs of life at the 2 week point, start to worry.

Or do it over again, this time with foil on the outside of the lid like the tek says - most legitimate teks (i.e. rated well in the FAQ) can't be improved upon much, other than little tweaks. Everything in them is there for a reason, follow what they say.

As for the viability of your spores, there almost certaintly still kickin' - I've used 2 year old spores before that showed growth on BRF in like 6 days. Don't get discouraged but don't be afraid to start over! Dedication will bring much to fruition.....


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Offlineblueferret
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Re: Tell me how bad I fucked my project up. [Re: Hypercube]
    #5252572 - 02/01/06 07:56 PM (17 years, 11 months ago)

Take the metal lids off or at least losten them so you get some air exchange. Aside from that just wait, back when I did BRF some of the jars would take up to 2 weeks to colinize.

Some advice though look into getting a PC and casing WBS. It is alot faster colonization, supplies are cheaper, and if you follow the teks personaly I have had alot less contamns.


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