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Offlinerequiem99
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Registered: 10/12/05
Posts: 35
Last seen: 17 years, 4 months
Tell me how bad I fucked my project up.
    #5247426 - 01/31/06 03:56 PM (18 years, 11 hours ago)

I'm following the PFTEK for simple minds as linked to on this site (currently 3 days into incubation and awaiting mycelial growth), with the following changes, all due to necessity and none to my own genius or "invention":

1) I did not use triple foil lids, I used single foil to sterilize the jars and then injected spores and immediately added sterile metal lids (no holes). Foil was sterlized prior to injecting with spores.

2) I sterlized using steam. I know this is iffy but I didn't have access to a pressure cooker OR a place to use it, so I boiled for 30-60 minutes, waited a day, then boiled again for 30-60 minutes a second time. The water ran out a few times and a few of the jars were subjected to dry heat, so this may have altered the water content -- I really don't know if it would since the jars were sealed. Someone can help me out on this one.

3) The spore syringes I used (1 syringer per 6 jars for 12 total) were PR and KS, not the best strains for a newbie but I didn't buy them when I knew what i was doing. That brings me to another thing: They've been kept in storage (a cool drawer sealed in the vacuum pack until 3 weeks ago) for 3 months since I bought them, and have been moved around in a bag to other locations 3 or 4 times. Does this mean I'm SOL and nothing will happen?

Also, do my jars need holes to incubate properly or are they fine as sealed?

I can post pics later on if anyone wants.


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Offlinerequiem99
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Registered: 10/12/05
Posts: 35
Last seen: 17 years, 4 months
Re: Tell me how bad I fucked my project up. [Re: Orranis]
    #5248312 - 01/31/06 07:32 PM (18 years, 7 hours ago)

I flamed the needle with a butane lighter AND a regular lighter after EACH jar and held my breath in a fairly sterile environment with as little airflow as possible.

I most certainly used the top layer of verm like the tek says. Thats one of the biggest keys from what I read.

RE FAE: How do I accomplish this? Right now some of my jars have that foggy condensation on them that comes back even if wiped off (weird shit). I have the lids on (screwed on top of the single layer of foil, which has 4 holes in it).


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Offlinerequiem99
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Registered: 10/12/05
Posts: 35
Last seen: 17 years, 4 months
Re: Tell me how bad I fucked my project up. [Re: requiem99]
    #5248377 - 01/31/06 07:43 PM (18 years, 7 hours ago)

As good a pic as I can get (fine grade vermiculite was used as you can tell):



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Offlinerequiem99
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Registered: 10/12/05
Posts: 35
Last seen: 17 years, 4 months
Re: Tell me how bad I fucked my project up. [Re: FooMan]
    #5248439 - 01/31/06 07:57 PM (18 years, 7 hours ago)

Quote:

FooManShroom said:
Your foil is under a metal lid? If so, you have NO gas exchange and you will have very slow growth or none at all. Poke some holes in the foil if there aren't some already.




There are 3-4 holes per foil lid, but the metal lid is on top of that, screwed on tight. Should it be loosened, or removed?


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Offlinerequiem99
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Registered: 10/12/05
Posts: 35
Last seen: 17 years, 4 months
Re: Tell me how bad I fucked my project up. [Re: coda]
    #5250530 - 02/01/06 10:06 AM (17 years, 11 months ago)

Sorry, like I said above, I couldn't follow the tek to the letter. I improvised due to circumstances.


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Offlinerequiem99
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Registered: 10/12/05
Posts: 35
Last seen: 17 years, 4 months
Re: Tell me how bad I fucked my project up. [Re: requiem99]
    #5252465 - 02/01/06 07:26 PM (17 years, 11 months ago)

Well its now day 5, and no mycelium OR mold growth yet. How long will it take to know that nothing is going to grow due to inadequate humidity?


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Offlinerequiem99
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Registered: 10/12/05
Posts: 35
Last seen: 17 years, 4 months
Re: Tell me how bad I fucked my project up. [Re: blueferret]
    #5253618 - 02/01/06 11:24 PM (17 years, 11 months ago)

I already loosened the lids and just laid them on top without screwing them on, so as to allow gas exchange. I did follow the tek as closely as I could (the foil inside the lid was my own invention because I had no way to get a sterile foil lid to cover the bottom lid where you make the injection), but I improvised where I had to.

Is there any way to easily notice if the jars have enough humidity? Some of them have condensation (almost looks like mold but its on the outside of the jars).


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Offlinerequiem99
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Registered: 10/12/05
Posts: 35
Last seen: 17 years, 4 months
Re: Tell me how bad I fucked my project up. [Re: requiem99]
    #5260876 - 02/03/06 09:35 PM (17 years, 11 months ago)

Day 6/7, one KS jar has a small green patch (argh), one KS jar has a medium white patch that looks like mycelial growth, and its growing fast. Will update after the weekend.


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