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Offlineendofmyrope
Stranger
Registered: 01/14/06
Posts: 7
Loc: on the ledge
Last seen: 17 years, 7 months
sending out an s.o.s.
    #5240315 - 01/29/06 09:38 PM (18 years, 1 month ago)

ive been lurking and learning here over 6mos and never thought id need to post but things are so bad i need to be talked of the ledge. my very first attempt at this was when i lived in a filthy apt w/a roomie. i got a very clumpy syringe from sporesdirect(now defunct) i did brf cakes in a pc for 1hr, lysol and ousted my room flamed/wiped the needle and threw the jars in my closet and within 3 days there was a thick white streak under every hole that quickly spread with beautiful ropy growth i got 3 oz's dry from 12 cakes i thought it was simple and gave away most to friends. now im in my own very clean place and i cant get healthy myc. to save my life. ive used 3 diff sponsored vendors for a total of 6 failures. each time now the myc starts only in small circular spots, the color is like cigar ash. good ropy myc will spread from these spots but the grey ash color follows it so that by the time its fully colonized there is 4 parts (under the holes)of grey pimply crap and 4 parts(in between the holes)that look o.k. most of the time i throw the jars out but once i fruited one just to see and it grew very small crappy shrooms, nothing like the monsters i had before. ive tried p.c.ing longer and shorter times, using a glove box, injecting in a closet full of lysol and oust wearing a mask, using a little more or less water in the jars, using bottled water, bleach soaking jars, using new jars, varying incubation temps. and its always the same. im going to fruit the 12 i have now no matter what to see what happens but im seriously getting sick of this. does anybody else get myc like this, my last idea is to inject outside on the trunk of my car in the winter temps which is crazy i know. please help somebody. i have no camera to show pics. sorry this was so long but im very desperate.

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OfflineCapCaptain
V.I.T.R.I.O.L.

Registered: 01/12/06
Posts: 187
Loc: The Matrix
Last seen: 17 years, 4 months
Re: sending out an s.o.s. [Re: endofmyrope]
    #5240434 - 01/29/06 10:11 PM (18 years, 1 month ago)

You could try doing everything in a Hepa filtered environment, maybe your new place have some unwanted contams.
Walgreens sells a really cheap Hepa for 39.99


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THERE IS NO SPOON
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My first trip VVVVVVVV
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5240648/an/0/page/0

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Offlineonetime
onetime
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Registered: 11/13/03
Posts: 3,609
Last seen: 13 years, 2 months
Re: sending out an s.o.s. [Re: CapCaptain]
    #5240489 - 01/29/06 10:37 PM (18 years, 1 month ago)

some cheap hepa's just collect larger amounts of contams and spit them back in the air. fyi. If  you get one then you would want to look in to makeing one. you can make a nuclear grade hepa system for around 50-75$ through ebay and your local hardware store. it all depends on the deal that you can find the hepa for. most of the time you can find a blower for uber cheap. then wood and glue and nails.

here is one for 25$

http://cgi.ebay.com/AstroCel-HCX-High-Ef...1QQcmdZViewItem


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See?
Yes, with my own three eyes.
Depression, Misspells , wanting everying thing i cant have haveing nothing i want

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InvisibleRahz
Alive Again
Male

Registered: 11/10/05
Posts: 9,260
Re: sending out an s.o.s. [Re: endofmyrope]
    #5242226 - 01/30/06 02:04 PM (18 years, 1 month ago)

I was waiting for a few responses, because I'm not sure what's causing the problem. If you think it's a contam problem, here's what I would suggest: Grab some RTV sealant at the auto supply store, grab some polyfil at walmart or wherever. Cover each of your inoculation holes with RTV sealant. Drill an additional 1/4" hole in the very center of the lid and stuff it with polyfil (make sure it's pretty tightly in there). The sealant will withstand your PC, so PC these jars with foil over them and you should have very contam resistant jars. The lids can be used many times. This is the best way I can think of for PF jars with mutiple inoculation points. I would also use a piece of tyvek under the lid to keep the polyfil from touching the substrate or verm barrier. A verm barrier shouldn't be necessary with these lids, but in order to rule out contams, it wouldn't hurt.

Rahz


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rahz

comfort pleasure power love truth awareness peace


"You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi

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Invisibletiny_rabid_birds
Nocturnal
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Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
Re: sending out an s.o.s. [Re: Rahz]
    #5242269 - 01/30/06 02:17 PM (18 years, 1 month ago)

I disagree Rahz.. I think that's too complicated for a simple PF tek jar.

endof, are you putting the inch thick layer of pure vermiculite on top of the substrate inside the jars, to act as a filter? What temperature is the closet that you incubate your jars in? Anything below mid 70s will cause extremely slow growth giving jars more time to be contamed.

I'm not sure what else to suggest. You said you flame and wipe the needle w/ alcohol, that's good. You PC at 15 psi for an hour, right? That's good. Has it been the same strain every time? Perhaps there's an odd mutation in the myc making it grow greyish.

Since you have a glovebox, perhaps you should read up on agar and do some petri dishes. That way you could isolate the healthy mycelium, then knock up some liquid culture jars. Hopefully that would allow you to get healthy mycelium growth and prevent you from having to buy any more spore syringes... since that shit can add up over time. Hopefully you can find out what the deal is, good luck w/ your grows man.


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Offlinecoda
Banjo Goiter
Male

Registered: 03/20/01
Posts: 8,750
Last seen: 11 months, 4 days
Trusted Cultivator
Re: sending out an s.o.s. [Re: tiny_rabid_birds]
    #5242330 - 01/30/06 02:30 PM (18 years, 1 month ago)

why not try a new substrate if you're having problems with BRF? You could also just be having shitty luck and are germinating crappy substrains. Have you looked into agar work and strain isolation?

You have a lot of choices available to you besides the normal PF tek, why not take a new route and see where you can go with it?

"You know what the definition of insanity is, lad? Its when you do the same bloody thing over and over and expect different results."


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To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.

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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: sending out an s.o.s. [Re: endofmyrope]
    #5242426 - 01/30/06 02:48 PM (18 years, 1 month ago)

What ever you decide to do...follow a tek to the "T".

Your either not doing something right...or you got ahold of some funky spore syringes.


I have a spore print for you.

Just send me a pm with your info and I'll send you one.

:smile:


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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InvisibleRahz
Alive Again
Male

Registered: 11/10/05
Posts: 9,260
Re: sending out an s.o.s. [Re: tiny_rabid_birds]
    #5242629 - 01/30/06 03:43 PM (18 years, 1 month ago)

>>>>I disagree Rahz.. I think that's too complicated for a simple PF tek jar.

Well, that's cool, it is complicated. If he's using mutiple syringe sources, going by the tek (with a PC no doubt), using something BULLET PROOF like I suggest would help to narrow down the problem.

If it wasn't for the fact that the grey starts so soon, and that he's varying the water ammounts, I would have guessed by the description that it's dry jars. A heating pad can cause this to happen, but I don't think that's what's going on since the jars continue to colonize after the grey shows up.

Post PC contam is the only thing I could think of (since he's using a PC), and he seems to be pretty careful with his inoculation procedure. I would lean twards contam during inoculation, but this is happening in every jar at every innoc point.

endofmyrope: What PSI and cooking times are you using? Also, some good pics would be helpful, borrow a camera if you can.

Using tyvek and inoculation ports on a PF jar is kinda crazy, but 6 trys with no success is crazy too.

Rahz


--------------------
rahz

comfort pleasure power love truth awareness peace


"You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi

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OfflineAngeloWish
Sr. Mydriasis

Registered: 07/13/05
Posts: 595
Loc: MEXICO-Mushroom Capital
Last seen: 4 years, 4 months
Re: sending out an s.o.s. [Re: Rahz]
    #5242677 - 01/30/06 03:58 PM (18 years, 1 month ago)

Build a glovebox! Works great!
I used to have every contam u can name... bacteria and multicolor molds and after the GB everything goes rite (i still get 10% of contams).

BTW. Myc is white, sometimes it can look gray cause vermiculite or substrate color but it will be white after all. Mushroom size varies from strain to strain and from tek to tek... I had similar thoughts when i saw my pf costaricans damn small (but potent enough)... now i am very happy with my poo gigants; mazatepec :smile:


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+'this' reality is the one i like the most+

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Offlineendofmyrope
Stranger
Registered: 01/14/06
Posts: 7
Loc: on the ledge
Last seen: 17 years, 7 months
Re: sending out an s.o.s. [Re: AngeloWish]
    #5244200 - 01/30/06 09:29 PM (18 years, 1 month ago)

something came to me last night. my first time i packed the jars very loose (per tek), in every try since i have been packing it in a little tighter in attempt to give them more "fuel" and kind of pressing down even more with the top verm. layer. looking at them today im noticing better growth where there are small air pockets. is it possible there's just not enough air and the myc. cant breathe/grow properly?
i do plan to try alot of those other methods you all mentioned but i want to get this right first before moving on. thanks everyone for the help.

Edited by endofmyrope (01/30/06 10:17 PM)

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Invisibletiny_rabid_birds
Nocturnal
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Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
Re: sending out an s.o.s. [Re: endofmyrope]
    #5244435 - 01/30/06 10:06 PM (18 years, 1 month ago)

That could definitely be the culprit. Airier/fluffier substrate is better, easier for the myc to colonize.


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Offlinebiglo
Shroomery BabySitter
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Folding@home Statistics
Registered: 11/22/02
Posts: 603
Loc: US of A
Last seen: 8 years, 6 months
Re: sending out an s.o.s. [Re: endofmyrope]
    #5244443 - 01/30/06 10:08 PM (18 years, 1 month ago)

First of all welcome!

As for your problems, yes, keeping your jars a bit less packed can help, makes the myc's job easier penetrating its food.  Also, try innoculating at two points in a jar instead of two.  It can help reduce chances of contams.  How big are the holes you made for the needles?  It should fit snug, if the holes are very large, you might be letting contams in.  I also adivse you to try putting RTV silicone sealant (look in the auto department of walmart, other places) to find it.  This will greatly reduce the risk of contams during innoc.  Hope this helps, I think you're on the right track, just having some shitty luck. :thumbup:

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Offlineendofmyrope
Stranger
Registered: 01/14/06
Posts: 7
Loc: on the ledge
Last seen: 17 years, 7 months
Re: sending out an s.o.s. [Re: Rahz]
    #5244739 - 01/30/06 11:14 PM (18 years, 1 month ago)

to answer everyone i have
-used the 1in verm. layer
-tried incubating at 76/80/82 with no diff.
-cooked at 15 psi for 45/60/70 min with no diff.
-my inoc. holes are very small
the 12 i have now dont seem as bad as others, it definitely isnt super white but it doesnt seem to be turning into creeping death yet.
?s
-could overpacked jars make myc.look grey cause verm. is to packed against glass and maybe this batch is o.k.
-the only grey cake i fruited gave very small shrooms. if it was contam. would it have fruited at all? they were texans. (thought everything was big in texas!)
-if i only inoc. in two holes wont colon. take longer (more time for contams?)

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