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InvisibleHyphalTryptamin
Stranger

Registered: 05/07/05
Posts: 390
Shake that myc
    #5242188 - 01/30/06 03:48 PM (15 years, 1 month ago)

Alright everyone, just a couple of general questions for ya

1) How prone is an LC to contaminate? when extracting fluid from the jar. Working in relatively sterile room, hole in tyvek the size of the needle, although i got some leakage because I had to put the jar on its side for the needle to submerge properly.

2) Does shaking vigorously with a needle hole  covered by tape pose an any threat to the LC?

3) Do the inoculation volumes of LC have to be more than that of multispore standard inoc? ie do you have to see chunks of white myc go in?
I'm just saying this because I might have stretched one 12ml LC syringe too far and the odd jar may have just been innoced with just malt water.

Thanks  :mushroom2:


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Invisibleagar
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Registered: 11/21/04
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Re: Shake that myc [Re: HyphalTryptamin]
    #5242231 - 01/30/06 04:06 PM (15 years, 1 month ago)





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OfflineFraggin
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Re: Shake that myc [Re: agar]
    #5242266 - 01/30/06 04:16 PM (15 years, 1 month ago)

Pictures worth 7,000 Words.


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OfflineRemainRandom50
Do You Need ToKnow Me?
Registered: 01/15/06
Posts: 1,695
Last seen: 11 years, 10 months
Re: Shake that myc [Re: Fraggin]
    #5242638 - 01/30/06 05:46 PM (15 years, 1 month ago)

fucking beautiful pictures above.
now what happens if someones end result to there LC did not look as cloudy as yours, but still has some cloudyness to it.

is this good, or basically malt water?


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At times I get consumed by my everyday life and will leave the Shroomery. Yet, every time drugs come falling into my life for fun.....I always think about the Shroomery and then I'm back!


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Offlineblackout
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Re: Shake that myc [Re: HyphalTryptamin]
    #5245934 - 01/31/06 11:11 AM (15 years, 30 days ago)

1. IME if you get a LC filter wet you are fucked, contams will grow through the filter, If it happens wipe with bleach of alcohol.

2. Yep if the filter gets wet.

3. You want the innoc to have visible myc. You can settle jars in a way so the myc settles at your insertion point. Or once sucked up (better to use a 60ml syringe) you can settle the syringe and squirt out the clear water first.


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InvisibleHyphalTryptamin
Stranger

Registered: 05/07/05
Posts: 390
Re: Shake that myc [Re: blackout]
    #5246489 - 01/31/06 01:35 PM (15 years, 30 days ago)

Cheers blackout bro - but I'm using tyvek - I think you're referring to polyfill though... Did already wipe holes with ethanol :thumbup:... But yes I think I will have to acquire a 30ml+ syringe. Do you have any idea in what kind of place I could get one?


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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