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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: Supalemonhaze] 1
#23387111 - 06/27/16 09:16 AM (8 years, 2 days ago) |
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Supalemonhaze said:
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PussyFart said: You still have to wait for the spores to germinate in the LC, and then test said LC for contams.....you save literally 0 time.

LC is easier to prepare and less intimidating. Its no quicker, but I never did test my LC before using it - using it was my test.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,893
Loc: Milky way
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] 1
#23387140 - 06/27/16 09:25 AM (8 years, 2 days ago) |
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Only reason agar work is intimidating likely is because a lot of the old hands were complete pussys about it for the longest time. Nothing we do in the entirety of shroom cultivation is hard. Children can do it, especially agar and pouring plates. LC sure a bit easier than pouring plates but both are easier than doing laundry.
If noobs are intimidated by agar and school failed to teach them the importance of looking for relevant, time tested, community supported information when researching then Idk they'll figure it out. They probably need to learn the hard way anyway
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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta] 1
#23387169 - 06/27/16 09:40 AM (8 years, 2 days ago) |
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Quote:
Trusted Cultivator said: Only reason agar work is intimidating likely is because a lot of the old hands were complete pussys about it for the longest time. Nothing we do in the entirety of shroom cultivation is hard. Children can do it, especially agar and pouring plates. LC sure a bit easier than pouring plates but both are easier than doing laundry.
If noobs are intimidated by agar and school failed to teach them the importance of looking for relevant, time tested, community supported information when researching then Idk they'll figure it out. They probably need to learn the hard way anyway
When i was a noob, LC worked out really well for me. That's why i kept using it.
If You look back At every post, i still agree agar is a better choice...
LC just works really well too. The proof is in the outcome. I still don't get why this upsets you guys so much? 
When i wrote this, no one was using gladware containers for agar. You had to get glass petri's, agar powder and a flow hood or SAB.
Everyone has a jar and some honey lying around...
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] 1
#23387303 - 06/27/16 10:34 AM (8 years, 2 days ago) |
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Mainly because this is bound to make some newbs crash and burn hard.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] 1
#23387375 - 06/27/16 10:56 AM (8 years, 2 days ago) |
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Quote:
_OttO_ said:
Quote:
Trusted Cultivator said: Only reason agar work is intimidating likely is because a lot of the old hands were complete pussys about it for the longest time. Nothing we do in the entirety of shroom cultivation is hard. Children can do it, especially agar and pouring plates. LC sure a bit easier than pouring plates but both are easier than doing laundry.
If noobs are intimidated by agar and school failed to teach them the importance of looking for relevant, time tested, community supported information when researching then Idk they'll figure it out. They probably need to learn the hard way anyway
When i was a noob, LC worked out really well for me. That's why i kept using it.
If You look back At every post, i still agree agar is a better choice...
LC just works really well too. The proof is in the outcome. I still don't get why this upsets you guys so much? 
When i wrote this, no one was using gladware containers for agar. You had to get glass petri's, agar powder and a flow hood or SAB.
Everyone has a jar and some honey lying around...
I used this because I didn't have a way to buy agar or a PC. I had a salsa jar and honey though. It was my first bulk try and kicked ass. Of course agar is better (duh) but LC's aren't obsolete like some people here think.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,893
Loc: Milky way
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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta] 1
#23387391 - 06/27/16 11:06 AM (8 years, 2 days ago) |
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You don't need a PC for agar or LC. But you do need a FH or SAB for either, even with SHIPs its a bad idea to open air
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta] 1
#23387408 - 06/27/16 11:15 AM (8 years, 2 days ago) |
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I used a crappy cardboard box for my first SAB
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 7 months, 19 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] 1
#23387606 - 06/27/16 12:31 PM (8 years, 2 days ago) |
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Quote:
_OttO_ said:I've used this method to grow for years with virtually no issues, so if it doesn't work for you, you are probably only going to get contaminated agar plates anyway.
I had great success in the past too, I wondered if all this talk of dirty spores was new vendors who supposedly buy off random people. I did have contams with an ex-sponsors ones, but have never had issues with 2 other sponsors, one now gone.
Some interesting comments from Ryche Hawk in this thread I started recently. Not sure if I can quote directly in mush cult so heres the link.
Extra clean spore syringes
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,885
Loc: Canada
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Re: A *beginner friendly* method for making Liquid Culture [Re: blackout] 1
#23387804 - 06/27/16 01:48 PM (8 years, 1 day ago) |
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You can grow with CFU's present. In fact to totally sterilize grain is rather impractical for the home hobbiest. In the end it's about populations. The more you start out with, the greater the chance for a bloom that overruns the colony or inhibits it.
With a syringe that had rather low CFU's it's very likely that one could put it right to liquid media and pull off a grow. I imagine that the amount of bacteria might not put the colony at risk of succumbing to mold during spawning. But it still hurts the BE in the end. So why not limit them CFU's as much as possible.
LC for me is not about stretching spores. It's about managing to inoculate large quantities of spawn. If I wanted to stretch a spore syringe I would shoot about 5cc of it into a half gallon of sterilized water. When I use LC it's because I want to do a good sized grow. I am probably wanting to be assured of stellar results which means using a familiar culture that I know is going to produce a big potent yield. Spores are a crap shoot, and contamination aside, I don't want to get a big bunk grow either. I want 200% BE and potent as fuck fruits.
I am certainly not an LC hater. I just feel that it's a powerful tool best wielded with precision.
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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta] 1
#23388436 - 06/27/16 05:26 PM (8 years, 1 day ago) |
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Quote:
Trusted Cultivator said: You don't need a PC for agar or LC. But you do need a FH or SAB for either, even with SHIPs its a bad idea to open air
The good thing about an LC jar is that you can loosen the lid, but leave it on - then just lift it a tiny crack to introduce a knife tip worth of spores from a spore print, in effect making the LC a mini SAB.
I never used a SAB and never had any dramas.
There are less vectors for contamination innoculating a LC than making a spore syringe, so it's a great option for going from print to media, without making syringes, and getting large amounts of innoculant quickly if all goes well.
Agar is still better for seeing contaminants in though.
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La Flama Blanca
Stranger

Registered: 01/15/16
Posts: 487
Last seen: 1 year, 3 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] 1
#23391095 - 06/28/16 01:13 PM (8 years, 23 hours ago) |
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Quote:
_OttO_ said:
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mupetmower said: i think what people are trying to say is that it's just more of a gamble, since it's hard to ensure a print is clean. especially from some vendor's spore syringes, which is what most noobs have. so taking the extra step to do agar first would help to ensure a clean culture, before potentially ruining a lot of jars, and discouraging someone.
EDIT - also since most noobs probably wont have the best sterile technique.
Almost all my prints came from members here, .
has the circle completed yet? are you now one of the members who has prints available?
(pls don't ban if its against rules to ask that)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: A *beginner friendly* method for making Liquid Culture [Re: La Flama Blanca] 1
#23391290 - 06/28/16 02:40 PM (8 years, 22 hours ago) |
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You should take a look at the marketplace if you want to trade spores.
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La Flama Blanca
Stranger

Registered: 01/15/16
Posts: 487
Last seen: 1 year, 3 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: Supalemonhaze] 1
#23391470 - 06/28/16 04:05 PM (8 years, 20 hours ago) |
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Wasn't aware of a marketplace here. i'll find it. thanks
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pesco
Stranger
Registered: 08/15/16
Posts: 1
Last seen: 7 years, 2 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: Sulli] 1
#23542868 - 08/15/16 03:35 AM (7 years, 10 months ago) |
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Sulli said: Well my LC is on day two looking very nice. Pics coming soon.
Also I stabed my finger with the needle trying to get the top off. Anyone else ever done that?
Please report the results. Would be good to know if inoculating a finger works as well as inoculating a log
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: A *beginner friendly* method for making Liquid Culture [Re: pesco] 1
#23543008 - 08/15/16 06:18 AM (7 years, 10 months ago) |
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Quote:
pesco said:
Quote:
Sulli said: Well my LC is on day two looking very nice. Pics coming soon.
Also I stabed my finger with the needle trying to get the top off. Anyone else ever done that?
Please report the results. Would be good to know if inoculating a finger works as well as inoculating a log
The person you're quoting hasn't been seen for months. What page is that post even on?
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: A *beginner friendly* method for making Liquid Culture [Re: Inocuole] 1
#23543108 - 08/15/16 07:26 AM (7 years, 10 months ago) |
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rofl.. 9 year old post that he quoted. page 8
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: A *beginner friendly* method for making Liquid Culture [Re: Inocuole] 1
#23543137 - 08/15/16 07:46 AM (7 years, 10 months ago) |
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Quote:
Inocuole said:
Quote:
pesco said:
Quote:
Sulli said: Well my LC is on day two looking very nice. Pics coming soon.
Also I stabed my finger with the needle trying to get the top off. Anyone else ever done that?
Please report the results. Would be good to know if inoculating a finger works as well as inoculating a log
The person you're quoting hasn't been seen for months. What page is that post even on?
pretty sure he's doin time
https://www.shroomery.org/forums/showflat.php/Number/22971336#22971336
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: A *beginner friendly* method for making Liquid Culture [Re: Munchauzen] 1
#23543704 - 08/15/16 11:24 AM (7 years, 10 months ago) |
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Well, at least his mold farm could have made for a good defense. He could tell the judge he was looking for a good mold to make blue cheese.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,893
Loc: Milky way
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Re: A *beginner friendly* method for making Liquid Culture [Re: Supalemonhaze] 1
#23543714 - 08/15/16 11:27 AM (7 years, 10 months ago) |
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most people never see jail time for mushrooms unless they're distributing bags of actual mushrooms, especially to kids.
depends on which part of the states you live in, more rural chances are more fucked though.
judging by that guys demeanor and posts he probably did himself no favors with the law though so...
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta] 1
#23544455 - 08/15/16 03:43 PM (7 years, 10 months ago) |
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Wow pulling that guy off the street probably reduced the ambient sporeload by at least 75%.
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