Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   OlympusMyco.com Olympus Myco Bulk Substrate   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < First | < Back | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | Next >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: wowimflabbergasted] * 1
    #23350735 - 06/16/16 11:32 AM (8 years, 13 days ago)

You still have to wait for the spores to germinate in the LC, and then test said LC for contams.....you save literally 0 time.

Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,867
Loc: ★
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart] * 1
    #23350741 - 06/16/16 11:34 AM (8 years, 13 days ago)

You only save time if you cycle your grows consistently.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,893
Loc: Milky way
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: Inocuole] * 1
    #23351105 - 06/16/16 01:30 PM (8 years, 12 days ago)

And if you do enough grows to make LC an attractive time saver you might as well just do a few more jars so you have a constant stream of work and then once again LC gets you no further ahead.

Extras: Filter Print Post Top
Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 6 months, 15 days
Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta] * 1
    #23352488 - 06/16/16 08:44 PM (8 years, 12 days ago)

I wonder how often a lc can appear 'clean' on a spawn jar but still be harboring bacteria...

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,885
Loc: Canada Flag
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: invitro] * 1
    #23352517 - 06/16/16 08:55 PM (8 years, 12 days ago)

Quote:

invitro said:
I wonder how often a lc can appear 'clean' on a spawn jar but still be harboring bacteria...




Its about populations. My last GLC attempt actually showed to be slightly bacterial when tested on agar. I used it anyway. Colonized fine and fruited well, only real indication was the BE was a bit low.

They moral is that bacteria can be present in a grow right from the inoculate and still work out. But it's still a real mold risk. So the answer to your question is that media can be harboring bacteria quite often and the grower may not suspect unless they are working with a known culture and the BE comes in low.

Extras: Filter Print Post Top
Offline_OttO_
Over Stimulated
 User Gallery


Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: Moabfighter] * 1
    #23370867 - 06/22/16 06:41 PM (8 years, 6 days ago)

Quote:

Moabfighter said:
Why would LC be better than just using a spore syringe?




You can expand a small amount of spores quickly to inoculate a lot more spawn.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,893
Loc: Milky way
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #23370943 - 06/22/16 07:04 PM (8 years, 6 days ago)

more people fit in a mine shaft elevator without their safety gear. the only ones attracted to this LC tek are noobs, anyone worth their salt makes their LC with a clean culture. people get upset because noobs gravitate towards this since it's attractive. if they can't figure out the good info from the bad with the overwhelming consensus let them get burned eventually

Extras: Filter Print Post Top
OfflineBobabouy
Shrimp Boat Captain
Male


Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 5 months
Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta] * 1
    #23370978 - 06/22/16 07:14 PM (8 years, 6 days ago)

I use LC quite a bit. But of course I always make it from a clean plate. Anything else is amateur hour. Maybe it will work and maybe it won't. And maybe it won't. Are you pickin up what I'm layin down? You smellin what I'm sellin?

Extras: Filter Print Post Top
Offline_OttO_
Over Stimulated
 User Gallery


Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: Bobabouy] * 1
    #23386923 - 06/27/16 08:09 AM (8 years, 2 days ago)

Quote:

Trusted Cultivator said:
more people fit in a mine shaft elevator without their safety gear. the only ones attracted to this LC tek are noobs, anyone worth their salt makes their LC with a clean culture. people get upset because noobs gravitate towards this since it's attractive. if they can't figure out the good info from the bad with the overwhelming consensus let them get burned eventually





:shrug:

There is only one difference here to inoculation of an agar plate with spores - that you can't see if it's contaminated before You use it.

If you use good technique, this really isn't an issue.

At worst case scenario you lose a few jars, which in reality could easily happen when transferring an agar wedge anyway, if your technique is not good.

All my LC inoculations were done open air, and i had probably less than 1% contamination issues.

I've used this method to grow for years with virtually no issues, so if it doesn't work for you, you are probably only going to get contaminated agar plates anyway.

BOTH LC and agar are awesome, with agar having the added benefit of being better for spotting contamination - but it can't fix your shitty sterile technique - so go suck a fat one, LC haters... :discodance: :ass:

The following pics were all achieved using LC inoculated in open air, from spore print or cloned tissue -

















Edited by _OttO_ (06/27/16 08:35 AM)

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #23386957 - 06/27/16 08:22 AM (8 years, 2 days ago)

So wait, everyone in this thread just has awful techniques? Not because spores are dirty, and 1 CFU of bacteria can fuck the whole LC up?

:goodluckwiththat:

LC is great, if you took the extra week to ensure it's clean. Starting up spores to LC is just stupid. Recommending it, and saying "if you need agar your technique sucks" is also stupid. It's this kinda misleading crap that everyone here is striving to fix on a daily basis.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
Offline_OttO_
Over Stimulated
 User Gallery


Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: Mad Season] * 1
    #23387014 - 06/27/16 08:39 AM (8 years, 2 days ago)

Quote:

Mad Season said:
starting up spores to LC is just stupid. Recommending it, and saying "if you need agar your technique sucks" is also stupid.




Its no different from going from spore print to agar except that you cant spot contamination. 99% of the time, this doesn't matter anyway.

There are less vectors for contamination than making a spore syringe.

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #23387038 - 06/27/16 08:47 AM (8 years, 2 days ago)

99% of the time? Really? God I must be unlucky because I'd say I see bacteria on my starting plates at least 30% of the time. For the longest time I couldn't grow until I cleaned up my shit on agar.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
Offline_OttO_
Over Stimulated
 User Gallery


Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: Mad Season] * 1
    #23387044 - 06/27/16 08:49 AM (8 years, 2 days ago)

Quote:

Mad Season said:
and saying "if you need agar your technique sucks" is also stupid.




I never said that. I said if your sterile technique is shit, agar isnt going to save you. It'll just prove your sterile technique is shit.

Agar is still the better choice, but a simple LC is also fantastic and very simple.

Extras: Filter Print Post Top
Offline_OttO_
Over Stimulated
 User Gallery


Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: Mad Season] * 1
    #23387053 - 06/27/16 08:52 AM (8 years, 2 days ago)

Quote:

Mad Season said:
99% of the time? Really? God I must be unlucky because I'd say I see bacteria on my starting plates at least 30% of the time. For the longest time I couldn't grow until I cleaned up my shit on agar.




I was always really careful, and found an almost perfect strike rate with LC. I can't confirm there were no bacterial contaminations, but I didnt notice any negative impact on any grows.

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #23387063 - 06/27/16 08:55 AM (8 years, 2 days ago)

Yeah I gotcha :wink:. I still don't get why people would want to start on LC rather than wedge > LC. It's like an extra couple weeks. But really IDC what people do, I was just like wtf when you said that success was dependant on good technique, because I know I have decent technique, and all my attempts were all shit until I had a clean inoculant


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
Invisiblemupetmower
Mower of Muppets
Male User Gallery


Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: A *beginner friendly* method for making Liquid Culture [Re: Mad Season] * 1
    #23387067 - 06/27/16 08:56 AM (8 years, 2 days ago)

wouldnt really matter how good your technique is, if the spores themselves are dirty, though. which is why starting on agar is much safer.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

Extras: Filter Print Post Top
Offline_OttO_
Over Stimulated
 User Gallery


Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: mupetmower] * 1
    #23387079 - 06/27/16 09:01 AM (8 years, 2 days ago)

Quote:

mupetmower said:
wouldnt really matter how good your technique is, if the spores themselves are dirty, though. which is why starting on agar is much safer.




Great point. Especially for wild prints.

Having said that, I started lots of wild prints in LC and still had no issues.

But agar is the obvious choice for peace of mind and professionalism.

Extras: Filter Print Post Top
Invisiblemupetmower
Mower of Muppets
Male User Gallery


Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #23387085 - 06/27/16 09:05 AM (8 years, 2 days ago)

i think what people are trying to say is that it's just more of a gamble, since it's hard to ensure a print is clean. especially from some vendor's spore syringes, which is what most noobs have. so taking the extra step to do agar first would help to ensure a clean culture, before potentially ruining a lot of jars, and discouraging someone.

EDIT - also since most noobs probably wont have the best sterile technique.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

Edited by mupetmower (06/27/16 09:07 AM)

Extras: Filter Print Post Top
InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart] * 1
    #23387088 - 06/27/16 09:07 AM (8 years, 2 days ago)

Quote:

PussyFart said:
You still have to wait for the spores to germinate in the LC, and then test said LC for contams.....you save literally 0 time.




:whathesaid:

Extras: Filter Print Post Top
Offline_OttO_
Over Stimulated
 User Gallery


Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: mupetmower] * 1
    #23387104 - 06/27/16 09:13 AM (8 years, 2 days ago)

Quote:

mupetmower said:
i think what people are trying to say is that it's just more of a gamble, since it's hard to ensure a print is clean. especially from some vendor's spore syringes, which is what most noobs have. so taking the extra step to do agar first would help to ensure a clean culture, before potentially ruining a lot of jars, and discouraging someone.

EDIT - also since most noobs probably wont have the best sterile technique.




I totally respect that.

Almost all my prints came from members here, and still I never had any issues. I can guarantee there were less than perfect conditions when people were taking prints, yet everything went well.

Agar is still better though.

Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | Next >

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   OlympusMyco.com Olympus Myco Bulk Substrate   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Ultimate Mycodirectory v4.0
( 1 2 3 4 all )
oOjonahOo 271,065 64 03/06/05 09:48 AM
by Anno
* THE STRAIN THREAD!
( 1 2 3 4 ... 21 22 )
Magician26 437,201 422 09/21/07 03:04 PM
by 2012shaman
* Filter Patch bags VS. Canning jars for substrate
( 1 2 3 all )
Ryche HawkV 27,734 40 01/24/19 01:27 PM
by bodhisatta
* Words in the wind SixTango 13,916 9 05/13/03 02:39 PM
by Robbyrob
* Oven Tek for Inoculation Jammer 14,734 15 04/06/19 04:05 PM
by bodhisatta
* CEREAL CAKES moorhsum 7,871 15 12/22/17 12:48 PM
by bodhisatta

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit
466,562 topic views. 0 members, 0 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.005 seconds on 13 queries.