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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 5 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: blackout]
#20792463 - 11/04/14 01:42 PM (9 years, 7 months ago) |
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nice post blackout, the whole floating thing is kinda out there but a neat idea i think it would be easier to do a no pour and simply squirt water on your colonized plate btu floating agar would be neat to pull off
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
#20794477 - 11/04/14 09:27 PM (9 years, 7 months ago) |
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Quote:
cronicr said: yeah sorry guys felt like shit for awhile but indeed both test jars were fine spawned both to coir and even made another glc from both got 20 cc's of each to use so i'm gonna knock some jars up asap
Nice to hear, and thanks for actually taking the time to experiment! I get the impression people who keep bagging LC's (I'm looking at you, pussyfart...), are just arguing over who has done more reading on the specifics of mushroom cultivation (more specifically who has read more of RR's posts), but don't actually put it into practice.
In my time I have made 100's of LC's from both spore prints and cloning tissue, the only difference is I pressure cook them now instead of boiling, and I have never had any problems.
I have grown a variety of strains and species with only 1 or 2 contammed LC's, ever. I dont ever shoot up test jars, I do all my innoculations in the open air, and have never encountered any issues yet.
Achieving mushroom cultivation on a hobby scale at home doesnt need to be complicated or scary, nor perfect - and that has been my whole intention from the word go - to encourage more people to try their hand playing with the hobby instead of being scared off by the technincal terminology and complicated sounding level of sterility/commitment, because I am 100% convinced there needs to be more mushrooms/psychedelic experiences in the world at the moment...
Here are some examples of grows I have done, all started using only spores or cloned tissue to -> LC (open air) made with this method -






I think the results speak for themselves, and we can put this one to bed now.
Peace
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#20794502 - 11/04/14 09:34 PM (9 years, 7 months ago) |
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bucket tek grows 
my second setup was a bunch of z-strain buckets that were the shit. 
Im about to do a bucket mono hybrid once I have all my supplies, its a 20gal bucket that Im going to set up like a monotub with a clear plexi glass lid to view threw during the whole process. I just need an area to work in and some coir/verm 
too busy with my
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: 13shrooms]
#20794630 - 11/04/14 10:13 PM (9 years, 7 months ago) |
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Quote:
13shrooms said: bucket tek grows 
my second setup was a bunch of z-strain buckets that were the shit. 
Im about to do a bucket mono hybrid once I have all my supplies, its a 20gal bucket that Im going to set up like a monotub with a clear plexi glass lid to view threw during the whole process. I just need an area to work in and some coir/verm 
too busy with my 
I love the ease of buckets to work with - so easy to clean and carry around, and you can isolate contams - with the added benefit of keeping gnats at bay...
A cheap shower cap makes a good cover instead of plexi-glass, and can be pulled up or down depending on what stage of growth you are at -

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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#20794649 - 11/04/14 10:17 PM (9 years, 7 months ago) |
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Man, that's awesome. Do those buckets get multiple flushes like other substrates? Are they set it and forget like monotubs?
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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
#20794696 - 11/04/14 10:28 PM (9 years, 7 months ago) |
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Quote:
stareatclouds said: Man, that's awesome. Do those buckets get multiple flushes like other substrates? Are they set it and forget like monotubs?
Yep, I get three flushes per bucket, abd they work on the same principal as monotubs, its pretty important to have some moving air in the room so a fan on low pointing away from them helps a lot.
I do mist a bit if they look dry, but you dont really need to...
They are very easy to dunk in the bucket after a flush, just harvest, add water to the bucket, drain and get ready for the next flush. Sooooo easy
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#20794819 - 11/04/14 11:03 PM (9 years, 7 months ago) |
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I dont think a shower cap will fit over a 20gal bucket 

they make great aquaponic cloners
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#20794829 - 11/04/14 11:06 PM (9 years, 7 months ago) |
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A shower cap that's brilliant! You rock Otto! Are there any holes for FAE in the cap or how does that work?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: A *beginner friendly* method for making Liquid Culture [Re: wowimflabbergasted]
#20794846 - 11/04/14 11:10 PM (9 years, 7 months ago) |
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Quote:
wowimflabbergasted said: A shower cap that's brilliant! You rock Otto! Are there any holes for FAE in the cap or how does that work?
I can see holes in the sides of the bucket with what looks like Tyvek or something. I'm guessing that's it?
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
#20794866 - 11/04/14 11:17 PM (9 years, 7 months ago) |
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holes at substrate level with micropore tape it looks to me and the shower cap "breaths" with tiny holes in it also.
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
#20794872 - 11/04/14 11:19 PM (9 years, 7 months ago) |
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I'm guessing that's micropore tape?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: A *beginner friendly* method for making Liquid Culture [Re: wowimflabbergasted]
#20795354 - 11/05/14 04:44 AM (9 years, 7 months ago) |
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Quote:
I do all my innoculations in the open air, and have never encountered any issues yet.
i do as well!
Quote:
I think the results speak for themselves, and we can put this one to bed now
otto did you ever try press n seal? stuffs great. it would stick well to the sides of the bucket. but im not sure about the height. you could make a loose piece and stick the sides to the bucket. although, no need really if the shower caps work! i used to do a box tek. where id take a regular box. line it with a trash bag, stick subs in it and do something very similar with the press n seal as a lid. super easy.
Edited by eatyualive (11/05/14 04:50 AM)
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#20796442 - 11/05/14 12:17 PM (9 years, 7 months ago) |
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Quote:
_OttO_ said: I get the impression people who keep bagging LC's (I'm looking at you, pussyfart...), are just arguing over who has done more reading on the specifics of mushroom cultivation (more specifically who has read more of RR's posts), but don't actually put it into practice.
I personally, through experiments and trial, have not had as good of luck with LC as I have with agar.
Quote:
_OttO_ said:I think the results speak for themselves, and we can put this one to bed now.
At least on your thread
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,893
Loc: Milky way
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Re: A *beginner friendly* method for making Liquid Culture [Re: SpitballJedi]
#20797137 - 11/05/14 03:06 PM (9 years, 7 months ago) |
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shhh you're not allowed to have negative views of LC here. it's off topic or something
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta]
#20797146 - 11/05/14 03:08 PM (9 years, 7 months ago) |
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: 13shrooms]
#20798148 - 11/05/14 06:26 PM (9 years, 7 months ago) |
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Quote:
13shrooms said: holes at substrate level with micropore tape it looks to me and the shower cap "breaths" with tiny holes in it also. 
There's no holes in the shower cap, the cap draws filtered air in through the micropore covered holes when you lift it up, in effect fanning your bucket with filtered air.
Quote:
wowimflabbergasted said: I'm guessing that's micropore tape?
Yep, there's four holes around the top, evenly spaced and two closer to substrate level, covered with micropore.
Quote:
SpitballJedi said: I personally, through experiments and trial, have not had as good of luck with LC as I have with agar.
Sorry to hear that, but at least you gave it a go, which is better than bagging something without trying it... Maybe your technique isnt up to scratch?
Ive always agreed that agar is still the preferred medium for spotting contaminations in your cultures, there's no argument there.... This thread was designed to encourage more people to try their hand at mycology, by showing how easy it can be.
This is because I have a fanciful belief the world needs more mushrooms, so I was trying to encourage people who might otherwise doubt their abilities or be turned off by complicated sounding techniques to get some fungi growing and have some mind expanding experiences for themselves and their friends.
Quote:
bodhisatta said: shhh you're not allowed to have negative views of LC here. it's off topic or something
Lol, I don't think he was being negative, just explaining his lack of success with LC's compared with when he uses agar.
And I only ever get frustrated at people missing the point of the thread, by getting weighed down by arguments over why agar is better.
Quote:
SpitballJedi said:
Quote:
_OttO_ said:I think the results speak for themselves, and we can put this one to bed now.
At least on your thread
Better luck next time... 
Quote:
PussyFart said:

I'm glad you're enjoying the show.... 
Edited by _OttO_ (11/05/14 06:36 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,893
Loc: Milky way
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#20798188 - 11/05/14 06:36 PM (9 years, 7 months ago) |
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it's more like even though it works I still would rather use agar for the reasons that are clear. I think it's good for someone not to skip over using agar as a tool until they get their first contamination. There's two LC users those who haven't had a contamination and those who have. With agar at least you know what you're doing before you try to run. LC can be a great tool. I don't argue against it in it's use for mass producing edible species stored as isolates and easily disseminated across amateur mycologists one of the best strains ever produced. But for cubes using agar is just as fast and effective if in rotation through out stages of growing even for a quite less modestly sized grow.
LC before agar is like not learning how to drive a manual as your first car. it really works for people to drive an automatic first but I prefer knowing how my car works.
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djmako7
Antitransubstantiationalist



Registered: 06/13/06
Posts: 2,362
Loc: A shady spot under a tree
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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta]
#20798200 - 11/05/14 06:37 PM (9 years, 7 months ago) |
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I'm trying out Monobuckets with my current grow. I was inspired by otto's monobuckets.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,893
Loc: Milky way
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Re: A *beginner friendly* method for making Liquid Culture [Re: djmako7]
#20798208 - 11/05/14 06:39 PM (9 years, 7 months ago) |
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buckets are great as monotubs. I've seen them pulled off really well.
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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,594
Loc: Up Over
Last seen: 12 days, 19 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#20798221 - 11/05/14 06:41 PM (9 years, 7 months ago) |
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Quote:
bodhisatta said: it's more like even though it works I still would rather use agar for the reasons that are clear. I think it's good for someone not to skip over using agar as a tool until they get their first contamination. There's two LC users those who haven't had a contamination and those who have. With agar at least you know what you're doing before you try to run. LC can be a great tool. I don't argue against it in it's use for mass producing edible species stored as isolates and easily disseminated across amateur mycologists one of the best strains ever produced. But for cubes using agar is just as fast and effective if in rotation through out stages of growing even for a quite less modestly sized grow.
LC before agar is like not learning how to drive a manual as your first car. it really works for people to drive an automatic first but I prefer knowing how my car works.
Quote:
_OttO_ said: And I only ever get frustrated at people missing the point of the thread, by getting weighed down by arguments over why agar is better.
Quote:
_OttO_ said: Ive always agreed that agar is still the preferred medium for spotting contaminations in your cultures, there's no argument there.... This thread was designed to encourage more people to try their hand at mycology, by showing how easy it can be.
This is because I have a fanciful belief the world needs more mushrooms, so I was trying to encourage people who might otherwise doubt their abilities or be turned off by complicated sounding techniques to get some fungi growing and have some mind expanding experiences for themselves and their friends.
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