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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: jamsubzero]
    #20695504 - 10/13/14 01:08 AM (9 years, 8 months ago)

Quote:

jamsubzero said:
oh, I thought the Tek said, "After a few days"..and "completely ready in a week"..



The tek is wrong.....that would be the absolute best case scenario.....and even then it's highly unlikely.

No one can know for sure how long your spores will take to germinate, or how fast the genetics will be.

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Offlinejamsubzero
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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
    #20695615 - 10/13/14 02:20 AM (9 years, 8 months ago)

Quote:

invitro said:
The usual case is a few days to germinate, I've never had one take longer than a week to get started.



Thanks, that clears a bit.

Quote:

PussyFart said:
Quote:

jamsubzero said:
oh, I thought the Tek said, "After a few days"..and "completely ready in a week"..



The tek is wrong.....that would be the absolute best case scenario.....and even then it's highly unlikely.

No one can know for sure how long your spores will take to germinate, or how fast the genetics will be.



Thanks man, that makes it clear for me even more, I thought I've done it completely bad. Now I just have to wait a few weeks to have my conclusion.

Another question, is it important for the jar LC to be in the dark to grow/germinate?

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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: jamsubzero]
    #20695627 - 10/13/14 02:25 AM (9 years, 8 months ago)

Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.

Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.

The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 83F , and contams thrive.

Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.


Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years.  Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger.
RR

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Offlineinvitro

Registered: 05/03/13
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Re: A *beginner friendly* method for making Liquid Culture [Re: jamsubzero]
    #20696040 - 10/13/14 08:45 AM (9 years, 8 months ago)

Quote:

jamsubzero said:
Quote:

invitro said:
The usual case is a few days to germinate, I've never had one take longer than a week to get started.



Thanks, that clears a bit.

Quote:

PussyFart said:
Quote:

jamsubzero said:
oh, I thought the Tek said, "After a few days"..and "completely ready in a week"..



The tek is wrong.....that would be the absolute best case scenario.....and even then it's highly unlikely.






LCs are more consistent in their take-off time than grains.  a few days is by far the norm. 

Edited by invitro (10/13/14 08:48 AM)

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Invisibleeatyualive
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Re: A *beginner friendly* method for making Liquid Culture [Re: invitro]
    #20699053 - 10/13/14 08:34 PM (9 years, 8 months ago)



--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Invisibleazur
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Re: A *beginner friendly* method for making Liquid Culture [Re: eatyualive]
    #20699078 - 10/13/14 08:38 PM (9 years, 8 months ago)

I love that thread eatyualive.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: jamsubzero]
    #20700026 - 10/14/14 12:42 AM (9 years, 8 months ago)

Quote:

jamsubzero said:
Quote:

invitro said:
The usual case is a few days to germinate, I've never had one take longer than a week to get started.



Thanks, that clears a bit.

Quote:

PussyFart said:
Quote:

jamsubzero said:
oh, I thought the Tek said, "After a few days"..and "completely ready in a week"..



The tek is wrong.....that would be the absolute best case scenario.....and even then it's highly unlikely.

No one can know for sure how long your spores will take to germinate, or how fast the genetics will be.



Thanks man, that makes it clear for me even more, I thought I've done it completely bad. Now I just have to wait a few weeks to have my conclusion.

Another question, is it important for the jar LC to be in the dark to grow/germinate?




No need to grow in the dark, but optimal temps will see yor LC grow much, much quicker, than fluctuating ambient room temperatures. A steady 25-27 degrees Celcius will see your Liquid culture flourish.

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Offlinedrgreen89
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #20743066 - 10/23/14 01:34 PM (9 years, 8 months ago)

[img]http://i00.i.aliimg.com/photo/v1/473166776/_X005_children_toothbrush_with_sucker_on.jpg_220x220.jpg[/]
this is how ive done my hole,

i chopped the bottom off this, (the sucker) so it was shaped >< like this

i cut a small hole in the middle of lid and i squeezed this thru as hard as i could so both sides has a lip over it, and the metal is cutting into the rubber.

i did my sterilising etc....lighter the needle and then stuck the needle thru and 2cc in,
out and it was sealed, and just to makesure on the rubber i melt the surface and press it with the lighter, totaly sealed and sterile, wrapped in a bastic  vac sealed bag and put onto my unit


--------------------
Using shrooms to treat PTSD mixed bipolar, and to help with restricted movements from severe back and neck injury
and research into Natural cures , Homeopathy

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OfflinecronicrFacebook
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Re: A *beginner friendly* method for making Liquid Culture [Re: drgreen89]
    #20743158 - 10/23/14 01:58 PM (9 years, 8 months ago)

ok i got two lc's ready to test out, soaking grains in the morning an i will update monday or so


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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InvisibleSpitballJedi
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Re: A *beginner friendly* method for making Liquid Culture [Re: 13shrooms]
    #20744492 - 10/23/14 07:17 PM (9 years, 8 months ago)

Quote:

13shrooms said:
look, I have the Q&A and I dont recommend half assed ways of doing anything that I wouldnt do myself BUT when a tek like this woks but you dont like the methods its personal preference.

I started here and moved to 2 or 3 different version b4 I found my reg methods, not everything works for everyone but we dont hinder them trying things out to learn for themselves.




What are your regular methods now and why did you move away from this one?


--------------------
The Basics
A little civility goes a long way

The Noob Forum
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Invisible13shroomsM
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Re: A *beginner friendly* method for making Liquid Culture [Re: SpitballJedi]
    #20745075 - 10/23/14 09:23 PM (9 years, 8 months ago)

Quote:

SpitballJedi said:
What are your regular methods now and why did you move away from this one?




see TLs "how I do everything" journal post or link in his sig..  Hes my understudy and everything he has written up or done (except his cig butt grow) on shroomery was by my direction.  so there you go.  :grin:

but if I were to start from scratch right this second I would shoot up a grain jar with a spore syringe then g2g (if its good to go) to get as much spawn as I needed then to a D5050 bucket tek in an ohmatic monotub. 

but that will change some once I have my work area set up and my flowhood built so I can start playing agar god with my PE :penis:

I changed from his tek because I was no longer a beginner without the good filtration supplies etc..  for using what you have around the house this tek along with the baby bottle tek for LCs were my go 2s 6 years ago..  :shrug:

learn - evolve - learn some more :nerd:

:getstoned:


--------------------
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Click here ^ for the AMU forum
VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV
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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
    #20745676 - 10/23/14 11:38 PM (9 years, 8 months ago)

Quote:

cronicr said:
ok i got two lc's ready to test out, soaking grains in the morning an i will update monday or so




Looking forward to seeing your progress... Did the LC's colonise ok?

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OfflinecronicrFacebook
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #20745962 - 10/24/14 03:09 AM (9 years, 8 months ago)

yeah they look fine:super:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Invisiblestareatclouds
star eat clouds?
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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
    #20762446 - 10/28/14 02:50 AM (9 years, 7 months ago)

I have an old glass jar with a thick plastic lid. Does the lid HAVE to be metal? I'll be boiling these instead of PCing them and it's a PP5 with the triangle so I believe it can withstand the heat of either PC or boiling.

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Invisibleeatyualive
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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
    #20762587 - 10/28/14 05:30 AM (9 years, 7 months ago)

Quote:

cronicr said:
yeah they look fine:super:



got any lc shots cronic?


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Offlinehamloaf
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Re: A *beginner friendly* method for making Liquid Culture [Re: eatyualive]
    #20762619 - 10/28/14 05:56 AM (9 years, 7 months ago)

I prefer to inoculate liquid mediums with proven-to-be-clean live culture from a petri dish, or a LC syringe from a reputable manufacturer.  I prefer to create my own clean culture though too.

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Invisibleazur
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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
    #20762767 - 10/28/14 07:11 AM (9 years, 7 months ago)

Quote:

stareatclouds said:
I have an old glass jar with a thick plastic lid. Does the lid HAVE to be metal? I'll be boiling these instead of PCing them and it's a PP5 with the triangle so I believe it can withstand the heat of either PC or boiling.



Dude, whatever you are using needs weight. That's why it works. Plastic will not work.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Invisiblestareatclouds
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Re: A *beginner friendly* method for making Liquid Culture [Re: azur]
    #20762785 - 10/28/14 07:18 AM (9 years, 7 months ago)

What needs weight? He used a salsa jar with a very thin metal top. I'm using an old glass jar which housed bouillon cubes. Unless you think I'm talking about the lid to the pot I boiled it in, I'm not sure what weight has to do with it, sorry.

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Invisibleazur
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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
    #20762792 - 10/28/14 07:20 AM (9 years, 7 months ago)

Oh shit. Forget that post. I was thinking about a thread of yours with stirrers in the lc and was thinking you wanted to break up the plastic lid to use for that. Woops.
Yes, plastic lid is fine. That's what i use


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
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Re: A *beginner friendly* method for making Liquid Culture [Re: azur]
    #20762797 - 10/28/14 07:22 AM (9 years, 7 months ago)

Haha, no worries (you f'ing piece of shit), but I think you're also confusing me with someone else with regard to that stirrer thread. Go drink some coffee, azur.

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