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Invisibleazur
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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
    #20670232 - 10/07/14 07:17 AM (9 years, 8 months ago)

I've done it too many times to count myself. It does work just fine. Though on occasion bacteria or yeast may be hiding.


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Offlinemidnightmaraude
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Re: A *beginner friendly* method for making Liquid Culture [Re: azur]
    #20670354 - 10/07/14 08:12 AM (9 years, 8 months ago)

Can you toss a colonized grain in the jar also?

And, can you just add the water and honey in the jar and put in the pc at 15psi for 20minutes to speed things up?

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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: midnightmaraude]
    #20670370 - 10/07/14 08:20 AM (9 years, 8 months ago)

Quote:

midnightmaraude said:
Can you toss a colonized grain in the jar also?

And, can you just add the water and honey in the jar and put in the pc at 15psi for 20minutes to speed things up?




Yes to both, but one of the rare times I got a contamination with a liquid culture was from inoculation with a colonized grain. Clone tissue or a core biopsy works well however, and I've had no troubles cloning using this method.

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Offlinemidnightmaraude
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #20670376 - 10/07/14 08:22 AM (9 years, 8 months ago)

thanks Otto

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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
    #20670384 - 10/07/14 08:26 AM (9 years, 8 months ago)

Quote:

cronicr said:
otto....just for u i'm gonna go to my kitchen tonight and do this:cool:




Now that's the attitude, be sure to post back your results!

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Offlinejsncrs
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #20681918 - 10/09/14 09:10 PM (9 years, 8 months ago)

When sucking up the mycelium from the jar, should you use a syringe with or without the needle? I didn't think the mycelium would be able to be sucked through a needle tip?

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Invisibleazur
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Re: A *beginner friendly* method for making Liquid Culture [Re: jsncrs]
    #20681975 - 10/09/14 09:28 PM (9 years, 8 months ago)

You need a llarge Guage needle. Large so it doesn't clog and needle so you can flame sterilize it. Though i personally pc my syringe with grain jars and just shoot it up


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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InvisibleMudaFuka
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Re: A *beginner friendly* method for making Liquid Culture [Re: azur]
    #20681993 - 10/09/14 09:33 PM (9 years, 8 months ago)

Is 16 guage big enough.


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AMU
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Invisibleazur
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Re: A *beginner friendly* method for making Liquid Culture [Re: MudaFuka]
    #20682002 - 10/09/14 09:39 PM (9 years, 8 months ago)

Quote:

MudaFuka said:
Is 16 guage big enough.



:thumbup:
Shake the lc well though


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Offlinejsncrs
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Re: A *beginner friendly* method for making Liquid Culture [Re: azur]
    #20682007 - 10/09/14 09:42 PM (9 years, 8 months ago)

Quote:

azur said:
You need a llarge Guage needle. Large so it doesn't clog and needle so you can flame sterilize it. Though i personally pc my syringe with grain jars and just shoot it up




Cool thanks pal

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InvisibleMudaFuka
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Re: A *beginner friendly* method for making Liquid Culture [Re: azur]
    #20682016 - 10/09/14 09:47 PM (9 years, 8 months ago)

Cool. I have a honey LC that I inoculated with an enoki wedge a while ago and just picked up some 60cc syringes last weekend. I put some broken glass in the LC jar so it should be easy enough to break up.


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AMU
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Liquid Inoculant Tek                                   
                     

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Invisibleazur
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Re: A *beginner friendly* method for making Liquid Culture [Re: MudaFuka]
    #20682195 - 10/09/14 10:52 PM (9 years, 8 months ago)

Broken glass or marbles work great. I seldom put anything in it.  I just swirl it.
I love seeing all the lc love that's coming around. I've been a fan for over a decade. A year ago on here everyone was so scared of it.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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InvisibleMudaFuka
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Re: A *beginner friendly* method for making Liquid Culture [Re: azur] * 1
    #20682264 - 10/09/14 11:14 PM (9 years, 8 months ago)

Shit comes and goes and the sheep just follow the herd.


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AMU
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Liquid Inoculant Tek                                   
                     

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OfflinecronicrFacebook
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Re: A *beginner friendly* method for making Liquid Culture [Re: MudaFuka]
    #20682290 - 10/09/14 11:23 PM (9 years, 8 months ago)

made two lc's for this both the same 4% honey in 1/4 pints one made like the tek states the other with a whatman a sab and my cambo culture:hehehe:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Invisibleazur
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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
    #20682304 - 10/09/14 11:27 PM (9 years, 8 months ago)

Hell yea cron


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Invisibleeatyualive
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Re: A *beginner friendly* method for making Liquid Culture [Re: MudaFuka]
    #20682869 - 10/10/14 05:05 AM (9 years, 8 months ago)

Quote:

MudaFuka said:
Shit comes and goes and the sheep just follow the herd.



:thumbup:


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Offlinejamsubzero
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Re: A *beginner friendly* method for making Liquid Culture [Re: eatyualive]
    #20695291 - 10/12/14 11:30 PM (9 years, 8 months ago)

How many days to get growth using spores?
Its been three days for mine and no result yet..

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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: jamsubzero]
    #20695325 - 10/12/14 11:44 PM (9 years, 8 months ago)

It can take up to 2+ weeks for germination to take place.

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Offlinejamsubzero
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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
    #20695366 - 10/13/14 12:05 AM (9 years, 8 months ago)

oh, I thought the Tek said, "After a few days"..and "completely ready in a week"..

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Offlineinvitro

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Re: A *beginner friendly* method for making Liquid Culture [Re: jamsubzero]
    #20695447 - 10/13/14 12:44 AM (9 years, 8 months ago)

The usual case is a few days to germinate, I've never had one take longer than a week to get started.

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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Golden Teacher Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   OlympusMyco.com Olympus Myco Bulk Substrate


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